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    A Comparative Study of Seminal Plasma and Blood Serum Macro and Trace Elements in the the Breeding (October) and the Non-Breeding (April) Seasons in Merino Ram
    (HELLENIC VETERINARY MEDICAL SOC, 2019) Bülbül, B.; Akalın, Pınar Peker; Başpınar, Nuri; Bucak, M. N.; Kırbaş, M.; Öztürk, C.; Güngör, S.
    In this study, it was aimed to investigate the concentrations of macro and trace elements in seminal plasma and blood serum in the breeding (October) and the non-breeding (April) seasons in Merino Ram. Nineteen Merino Rams, aged 18-24 months, were involved in the study. Blood (once) and ejaculate samples (6 replicates) were taken in the breeding (October) and the non-breeding (April) seasons. Blood serum, seminal plasma and diet Calcium, Sodium, Potassium, Magnesium, Phosphorus, Sulfur, Zinc, Selenium, Chrome, Manganese, Nickel, Molybdenum and Boron concentrations were determined by ICP-AES. In blood serum, Sodium and Selenium concentrations were higher (p<0.05 and 0.001, respectively) in the the breeding season than in non-breeding season, whereas Potassium, Chromium and Boron concentrations were lower (p<0.05, 0.001 and 0.001, respectively) in the breeding season than in the non-breeding season. In seminal plasma Calcium, Sodium, Zinc and Manganese concentrations were higher (p<0.05, 0.001, 0.01 and 0.05, respectively) in the breeding season than in the non-breeding season, whereas Phosphorus, Chrome, Molybdenum and Boron concentrations were lower (p<0.001, 0.001, 0.05 and 0.001, respectively) in the breeding season than in the non-breeding season. No difference was detected regarding the other elements. The higher levels of Cr and B in the non-breeding season compared to the breeding season both in serum and seminal plasma, regardless of diet intake, suggest that these elements may play a crutial role on male fertility in Merino Ram.
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    Effect of Parity on Oestrus Synchronization Success in Cows
    (Ecole Nationale Veterinaire Toulouse, 2006) Bülbül, B.; Ataman, M. B.
    The objective of the present study wits to evaluate the effect of parity on pregnancy rate in lactating Holstein cows Subjected to different synchronization protocols. A total of 96 cows (49 primiparous and 47 multiparous) were allocated randomly into four groups containing primiparous and multiparous COWS. In the PRID group (n=23), progesterone releasing intravaginal device (PRID) was inserted into anterior vagina of the cows. The devices Were removed 12 days later and 500 mu g of cloprostenol was intramuscularly administered the day before PRID withdrawal. In the Implant group (n=25), ear implants releasing norgestomet were Subcutaneously g inserted in the Car of each cow for 9 days and 500 mu g of cloprostenol was intramuscularly administered the day before the implant removal. In the GnRH group (n=24), buserelin, a GnRH analogue 10 mu g was given oil day 0 followed by the administration of 500 mu g of cloprostenol, 7 days later. In the PGF(2 alpha) group (n=24), two intramuscular administrations of 500 mu g of cloprostenol were performed at an 11-day interval. All cows exhibited oestrus signs between 36 and 124 h after the last PGF(2 alpha) administration. Mean overall pregnancy rate of the cows from the GnRH group Wits lower that that of the cows from the PRID, Implant and PGF(2 alpha) groups which were 86.9%, 72.0%1 54.2% and 83.3%, respectively. Mean pregnancy rates of the primiparous cows were not influenced by the treatment and were 83.3%, 73.3% 63.6% and 81.8% in the PRID. Implant, GnRH and PGF(2 alpha) groups. Mean pregnancy rate of the multiparous cows of the GnRH group (46.1%) was lower than that of the cows from the PRID (90.9%), and PGF(2 alpha) (84.6%) groups but was not different from that observed for the Implant group (70.0%). In conclusion. the synchronization protocols associating progesterone and PGF(2 alpha) or 2 PGF(2 alpha) administrations were more efficient than the treatment associating buserelin and PGF(2 alpha) whatever the cow's parity.