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    Histomorphometric examination of the pineal gland in foals and adult horses
    (Chartered Inst. of Building Services Engineers, 2018) Bolat D.; Kürüm A.; Bahar S.; Karahan S.
    This study was conducted to evaluate the pineal glands of the foal and adult horses with histomorphometry. The pineal glands were sectioned at a thickness of 40 µm and stained with AgNOR for stereological analyses. The weight and volume of the pineal gland as well as the number of pinealocytes were significantly higher in the adult horses (P=0.009). However, the number of pinealocytes in per volume was similar between foals and adult horses. Such data indicate that growth in the size of the gland is related to increase in the number of pinealocytes. The pinealocyte nucleus is significantly larger in adults (P= 0.009). Such a size difference should be further investigated if it is due to an increase in the number of cells with increased DNA content. Melanin was distributed throughout the foal pineal gland whereas it was focally localized to connective tissue in adults. The different patterns in melanin distribution suggest that foals and adult horses may differ by means of melanin metabolism in the pineal gland. © 2018, Chartered Inst. of Building Services Engineers. All rights reserved.
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    Selective gray and white matter staining of the horse spinal cord
    (Veteriner Fakultesi Dergisi, 2012) Bolat D.; Bahar S.; Sur E.; Selcuk M.L.; Tipirdamaz S.
    The ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 ?m sections to be stained separately with the different staining methods. Six different staining methods, including Modified May - Grunwald - Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson's trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.