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    Marker Assisted Selection for Photoperiod Insensitive Ppd-D1a Allele in Winter Wheat Breeding Program
    (ELSEVIER SCIENCE BV, 2012) Akman, Hayati; Bruckner, Phil
    This study was conducted in Spring Wheat Lab of Montana State University. Marker assisted selection is a useful molecular breeding method to improve the efficiency and precision of conventional plant breeding. The Ppd-D1a allele of 49 lines in F2 generation and the 4 parents Yellowstone, NE01533, Pelsart and Promontory were determined using microsatellite markers. Breeders can efficiently use this molecular information in their breeding programs by adding and removing photoperiod insensitive Ppd-D1a alleles to their varieties. In hexaploid wheat, photoperiod insensitive PpdD1a allele in 2D chromosome causes early flowering in short day length and long day length, avoiding stresses associated with high temperature and water deficit in grain filling stages. This study shows that the parents NE01533, Pelsart, Promontory and the 47 lines in F2 generation have the Ppd-D1a allele while Yellowstone and the remaining lines are photoperiod sensitive.
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    MARKER ASSISTED SELECTION FOR RHT8 AND RHT-D1B DWARFING GENES IN WINTER WHEAT BREEDING PROGRAM
    (HEALTH & ENVIRONMENT ASSOC, 2016) Akman, Hayati; Bruckner, Phil
    Plant height has been shown to reduce from 3.49 to 12.5% for Rht8 and 17 to 24 % for Rht-D1b in many studies. Several studies have indicated that yield increases from 3.8 to 12% for Rht8 and 16 to 30 % for Rht-D1b. Breeders can efficiently use this molecular information in their breeding programs by adding Rht8 and Rht-D1b alleles. Marker assisted selection is a useful molecular breeding method to improve the efficiency and precision of conventional plant breeding, which were used to determine Rht8 and Rht-D1b alleles in 49 lines in F2 generation and the 4 parents Yellowstone, NE01533, Pelsart, Promontory. This research was conducted in Spring Wheat Genetic Lab of Montana State University. Presence of the Rht8 dwarfing gene was determined by genotyping alleles at the GWM261F and GWM261R microsatellite markers locus chromosomally linked to Rht8. DF-MR2 detects mutant Rht-D1b dwarfing allele. This reseach showed that parent NE01533 and 20 lines in F2 generation had Rht-D1b dwarfing gene while 18 lines in F2 generation had Rht8 dwarfing gene.
  • Küçük Resim
    Öğe
    Marker Assisted Selection for Rht8 and Rht-D1b Dwarfing Genes in Winter Wheat Breeding Program
    (ELSEVIER SCIENCE BV, 2012) Akman, Hayati; Bruckner, Phil
    Plant height has been shown to reduce from 3.49 to 12.5% for Rht8 and 17 to 24% for Rht-D1b in many studies. Several studies have indicated that yield increases from 3.8 to 12% for Rht8 and 16 to 30% for RhtD1b. Breeders can efficiently use this molecular information in their breeding programs by adding Rht8 and PpdD1b alleles. Marker assisted selection is a useful molecular breeding method to improve the efficiency and precision of conventional plant breeding, which are used to determine Rht8 and Ppd-D1b alleles in 49 lines in F2 generation and the 4 parents Yellowstone, NE01533, Pelsart, Promontory. This research was conducted in Spring Wheat Genetic Lab of Montana State University. Presence of the Rht8 dwarfing gene was determined by genotyping alleles at the gwm261R and gwm261L microsatellite markers locus chromosomally linked to Rht8. DF-MR2 detects mutant Rht-D1b dwarfing allele. This research shows that parent NE01533 and 20 lines in F2 generation have Ppd-D1b dwarfing gene while 18 lines in F2 generation have Rht8 dwarfing gene.