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    Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull
    (WILEY, 2015) Sariozkan, S.; Tuncer, P. B.; Buyukleblebici, S.; Bucak, M. N.; Canturk, F.; Eken, A.
    The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5mm), hyaluronan (0.25, 1mgml(-1)) and fetuin (5, 10mgml(-1)) in the freezing of Brown Swiss bull semen. The best percentages of CASA motilities were achieved with 10mgml(-1) of fetuin and 2.5mm of cysteamine. For sperm morphology, 10mgml(-1) of fetuin and 2.5mm of cysteamine had better protective effects (P<0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5mgml(-1) of fetuin, were higher than those of the control group (P<0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25mgml(-1) of hyaluronan, and 2.5 and 7.5mm of cysteamine led to lower rates of spermatozoa with damaged DNA, compared with the control group (P<0.001). The maintenance of superoxide dismutase and glutathione peroxidase antioxidant activities following freeze-thawing with 2.5 and 7.5mm of cysteamine and 10mgml(-1) of fetuin was demonstrated to be at a higher level in comparison with the control group (P<0.001). Malondialdehyde formation was found to be lower in the groups supplemented with 0.25mgml(-1) of hyaluronan and 7.5mm of cysteamine after the freeze-thawing process (P<0.001).
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    A Comparative Study of Seminal Plasma and Blood Serum Macro and Trace Elements in the the Breeding (October) and the Non-Breeding (April) Seasons in Merino Ram
    (HELLENIC VETERINARY MEDICAL SOC, 2019) Bülbül, B.; Akalın, Pınar Peker; Başpınar, Nuri; Bucak, M. N.; Kırbaş, M.; Öztürk, C.; Güngör, S.
    In this study, it was aimed to investigate the concentrations of macro and trace elements in seminal plasma and blood serum in the breeding (October) and the non-breeding (April) seasons in Merino Ram. Nineteen Merino Rams, aged 18-24 months, were involved in the study. Blood (once) and ejaculate samples (6 replicates) were taken in the breeding (October) and the non-breeding (April) seasons. Blood serum, seminal plasma and diet Calcium, Sodium, Potassium, Magnesium, Phosphorus, Sulfur, Zinc, Selenium, Chrome, Manganese, Nickel, Molybdenum and Boron concentrations were determined by ICP-AES. In blood serum, Sodium and Selenium concentrations were higher (p<0.05 and 0.001, respectively) in the the breeding season than in non-breeding season, whereas Potassium, Chromium and Boron concentrations were lower (p<0.05, 0.001 and 0.001, respectively) in the breeding season than in the non-breeding season. In seminal plasma Calcium, Sodium, Zinc and Manganese concentrations were higher (p<0.05, 0.001, 0.01 and 0.05, respectively) in the breeding season than in the non-breeding season, whereas Phosphorus, Chrome, Molybdenum and Boron concentrations were lower (p<0.001, 0.001, 0.05 and 0.001, respectively) in the breeding season than in the non-breeding season. No difference was detected regarding the other elements. The higher levels of Cr and B in the non-breeding season compared to the breeding season both in serum and seminal plasma, regardless of diet intake, suggest that these elements may play a crutial role on male fertility in Merino Ram.
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    Effects of Basal Medium Eagle (BME) on Anatolian Shepherd (Kangal) dog sperm freezing
    (WILEY-BLACKWELL, 2015) Gungor, S.; Bucak, M. N.
    [Abstract not Available]
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    Effects of curcumin and dithioerythritol on frozen-thawed bovine semen
    (WILEY, 2012) Bucak, M. N.; Başpınar, Nuri; Tuncer, P. B.; Coyan, K.; Sarıozkan, S.; Akalın, Pınar Peker; Büyükleblebici, S.
    The aim of this study was to determine the effects of curcumin and dithioerythritol added into bull semen extender on sperm parameters, lipid peroxidation, total glutathione and antioxidant potential levels of bull spermatozoa following the freeze/thawing process. Twenty-seven ejaculates obtained from three bulls were included in the study. Each ejaculate that was splitted into five equal groups and diluted in a Tris-based extender containing curcumin (0.5 and 2 mm), dithioerythritol (0.5 and 2 mm) and no additive (control) was cooled to 5 degrees C and frozen in 0.25-ml French straws. The extender supplemented with 0.5 mm dose of curcumin led to lower percentage of total abnormality (20.40 +/- 2.36%) when compared to the control (30.60 +/- 1.47%, P < 0.05). Curcumin and dithioerythritol at 0.5 mm provided a greater protective effect in the membrane functional integrity (54.40 +/- 2.09% and 50.00 +/- 2.68%), in comparison with control (37.20 +/- 1.77%, P < 0.001). Supplementation with antioxidants did not significantly affect the lipid peroxidation and antioxidant potential levels, while the maintenance of total glutathione levels in curcumin 0.5 mm was demonstrated to be higher than that of control, following the freeze/thawing (P < 0.05). Supplementation with these antioxidants prior to the cryopreservation process may be recommended to facilitate the enhancement of sperm cryopreservation techniques.
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    Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate in Merino ram
    (WILEY-BLACKWELL, 2015) Akalın, Pınar Peker; Coyan, K.; Bucak, M. N.; Güngör, U.; Öztürk, C.
    [Abstract not Available]
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    Impacts of oxidative stress and antioxidants on ram and goat semen functions
    (WILEY-BLACKWELL, 2011) Bucak, M. N.
    [Abstract not Available]
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    Lycopene and resveratrol improve post-thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity
    (WILEY-BLACKWELL, 2015) Bucak, M. N.; Ataman, M. B.; Baspinar, N.; Uysal, O.; Taspinar, M.; Bilgili, A.; Ozturk, C.
    We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 degrees C with base extenders containing lycopene (1x10(-3)gml(-1)) and resveratrol (1mm), and no antioxidant (control), was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 +/- 3.8 and 61.9 +/- 4.0%) and progressive motility (38 +/- 2.4 and 37 +/- 8.8), compared with the controls (50.7 +/- 2.65 and 33.3 +/- 3.74%, respectively, P<0.05). Resveratrol provided a higher ALH (4.3 +/- 0.1), in comparison with the control (3.9 +/- 0.3, P<0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 +/- 2.9 and 30.1 +/- 6.5% respectively), compared with that of the control (11.8 +/- 9.5%, P<0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P<0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.