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    Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull
    (WILEY, 2015) Sariozkan, S.; Tuncer, P. B.; Buyukleblebici, S.; Bucak, M. N.; Canturk, F.; Eken, A.
    The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5mm), hyaluronan (0.25, 1mgml(-1)) and fetuin (5, 10mgml(-1)) in the freezing of Brown Swiss bull semen. The best percentages of CASA motilities were achieved with 10mgml(-1) of fetuin and 2.5mm of cysteamine. For sperm morphology, 10mgml(-1) of fetuin and 2.5mm of cysteamine had better protective effects (P<0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5mgml(-1) of fetuin, were higher than those of the control group (P<0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25mgml(-1) of hyaluronan, and 2.5 and 7.5mm of cysteamine led to lower rates of spermatozoa with damaged DNA, compared with the control group (P<0.001). The maintenance of superoxide dismutase and glutathione peroxidase antioxidant activities following freeze-thawing with 2.5 and 7.5mm of cysteamine and 10mgml(-1) of fetuin was demonstrated to be at a higher level in comparison with the control group (P<0.001). Malondialdehyde formation was found to be lower in the groups supplemented with 0.25mgml(-1) of hyaluronan and 7.5mm of cysteamine after the freeze-thawing process (P<0.001).
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    Effects of some cryoprotectants and antioxidants on frozen/thawed bull sperm motility and motion characteristics
    (WILEY-BLACKWELL, 2011) Tuncer, P. B.; Bucak, M. Numan; Buyukleblebici, S.; Kuzuguden, F.; Sariozkan, S.; Tasdemir, U.; Kinet, H.
    [Abstract not Available]