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Öğe Comparing ethylene glycol with glycerol and with or without dithiothreitol and sucrose for cryopreservation of bull semen in egg-yolk containing extenders(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014) Buyukleblebici, Serhat; Tuncer, Purhan Barbaros; Bucak, Mustafa N.; Tasdemir, Umut; Eken, Ayse; Buyukleblebici, Olga; Durmaz, EmreThere are few studies performed for investigating the roles of different ratio and cryoprotectants with dithiothreitol or sucrose on sperm motility characteristics and antioxidant capacities of post-thawed bull spermatozoa. The objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations as cryoprotectants and dithiothreitol (D) or sucrose (S) (with/without) as antioxidants in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was split into four equal aliquots and diluted using both of the Tris extenders with glycerol (5% or 7%) or ethylene glycol (3% or 5%). After that, each extenders were split into three equal aliquots and diluted using both of the dithiothreitol 5 mM or sucrose 25 mM, and control (without additives) was cooled to 4 degrees C and frozen in 0.25-ml French straws. when compared to control, different doses cryoprotectants and antioxidants addition no significantly increased the percentages of post-thaw sperm progressive and motitilities, acrosome abnormality and plasma membrane integrity (P > 0.05). However, EG3 + S yielded the greatest percentages of the total abnormality (P < 0.05). As regard to antioxidant activities G7 and EG5 led to lowest MDA activity with or without D or S but, these results were not supported to the GPx activity (P < 0.01). The sperm motion characteristics such as VAP, VCL, ALH and BCF gave significantly different results (P < 0.05). When compared the DNA integrity, different doses cryoprotectants without antioxidants addition significantly increased the percentages the tail intensity and tail moment (P < 0.05). There were no significant differences observed in non-return rates among all treatment groups (P > 0.05). (C) 2014 Elsevier Inc. All rights reserved.Öğe Cryopreservation of bull sperm: Effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results(ELSEVIER, 2014) Buyukleblebici, Serhat; Tuncer, Purhan Barbaros; Bucak, Mustafa Numan; Eken, Ayse; Sariozkan, Serpil; Tasdemir, Umut; Endirlik, Burcu UnluThe objectives of this study were to compare glycerol (G) and ethylene glycol (EG) at different concentrations and trehalose (T) or cysteine (C; with/without) in Tris extender for cryopreservation of bull semen. Twenty-four ejaculates obtained from three bulls were included in the study. Each ejaculate was divided into four equal aliquots and diluted using both of the Tris extenders with G (5% or 7%) or EG (3% or 5%). After that, each extenders were divided into three equal aliquots and diluted using both of the 5 mM C or 25 mM T, and control (without additives) was cooled to 4 degrees C and frozen in 0.25 ml French straws. The addition of 3% and 5% EG without antioxidants resulted in the least Computer-Assisted Sperm motility Analysis (CASA) motility as compared with the other groups. Treatment with 25 mM Tin 3% EG beneficially effected acrosome morphology as compared with the other groups. Also, treatment with 3% EG with 25 mM T and 5% EG resulted in a greater rate of total abnormalities. Treatment with 3% G yielded a slightly greater percentage of membrane integrity by Hypo-Osmotic Swelling Test (HOST) assessment than that of the other groups. Treatment with 3% EG with 5 mM C resulted in the greatest concentration of malondialdehyde (MDA). The glutathione peroxidase (GPx) antioxidant activity was increased in the C-treatment groups when compared to the other groups. Treatment with 5% EG and 5 mM C resulted in less chromatin damage and detrimental impacts on tail moment. Treatment with 5% EG led to greater non-return rates of inseminated cows. However, this result was not considered to be statistically important. (C) 2014 Elsevier B.V. All rights reserved.Öğe The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen(Selçuk Üniversitesi Veterinerlik Fakültesi, 2010) Sariozkan, Serpil; Tuncer, Purhan Barbaros; Bucak, Mustafa Numan; Buyukleblebici, Serhat; Kinet, Huseyin; Bilgen, AliAmaç: Boğa spermasını dondurmak için soya lesitin temelli sulandırıcıya (Bioxcell®) %5 ve 10 konsantrasyonlarında santrifüj edilmiş yumurta sarısı (SYS) katıldı ve bunların çözüm sonu sperm motilite, morfolojik anormallikler ve membran bütünlüğü üzerindeki sinerjistik etkileri değerlendirildi. Gereç ve Yöntem: Her bir Simental boğadan alınan ejakulatlar (n=12) üç eşit miktara ayrıldı ve sırasıyla %5 (B5), %10 (B10) SYS eklenmiş ve hiç SYS (B0) içermeyen soya lesitin temelli sulandırıcı ile sulandırıldı. Ardından standart protokollere göre donduruldu ve çözdürüldü. Spermatozoa kryocanlılığı, in vitro çözüm sonu motilite (CASA), akrozomal ve diğer anormallikler ve plasma membran bütünlüğü (HOST) yönünden değerlendirildi. Bulgular: Simental boğalarda, dondurma ve çözdürme sonrası B5 ile sulandırılan grupta, diğer sulandırıcılarla sulandırılan gruplardan önemli derecede daha yüksek CASA motilite ve CASA progresif motilite oranı elde edilmiştir (p<0.001). Gruplar arasında, VAP, VCL ve ALH yönünden önemli bir farklılık bulunmamıştır (p>0.05). En yüksek VSL (p<0.01) ve LIN değeri (p<0.001) B10 grubundan elde edilmiştir. Membran bütünlüğü oranı, B5 grubunda, diğer gruplara göre önemli derecede yüksek bulunmuştur (p<0.001). Öneri: Soya lesitinle kombine olarak sulandırıcıya %5 SYS eklenmesi boğa spermasının dondurulabilirliğini önemli derecede artırmıştır.Öğe Evaluation of post-thaw quality of Brown-Swiss and Holstein bull semen diluted with different extenders(Selçuk Üniversitesi Veterinerlik Fakültesi, 2010) Sariozkan, Serpil; Tuncer, Purhan Barbaros; Bucak, Mustafa Numan; Buyukleblebici, Serhat; Kinet, Huseyin; Bilgen, AliAmaç: Tris + yumurta sarısı, Bioxcell® ve Optidyl® sulandırıcıları ile sulandırılan İsviçre-Esmeri ve Holştayn boğa spermasının çözdürme sonrası kalitesini değerlendirmektir. Gereç ve Yöntem: Holştayn (n=36) ve Esmer (n=36) boğalardan alınan ejakulatlar üç kısma ayrıldı ve sırasıyla Tris, Optidyl® ve Bioxcell® sulandırıcılarıyla sulandırıldı. Standart protokollere göre donduruldu ve çözdürüldü. Sulandırıcıların etkinliği, çözüm sonu sperm motilitesi, akrozomal ve toplam anormallikler ve plazma membran bütünlüğü değerlendirilerek belirlendi. Bulgular: Holştayn ırkı boğa spermasında, dondurmaçözdürme sonrası en yüksek subjektif (p<0.01), ilerleyen (p<0.001) ve CASA motilite (p<0.001) oranları Optidyl® ile sulandırılan grupta saptanmıştır. Optidyl® sulandırıcısının spermatozoa akrozom ve membran bütünlüğünü diğer sulandırıcılara kıyasla en iyi şekilde koruduğu saptanmıştır (p<0.001). Motilite karakterlerinden VAP, VSL ve LIN yönünden en yüksek değerler Optidyl ve Tris sulandırıcılarından elde edilmiştir (p<0.05). Esmer ırk boğa spermasında ise, sulandırıcılar arasında en düşük çözüm sonu subjektif (p<0.01), CASA (p<0.001) motiliteleri ve membran bütünlüğü (p<0.001) oranı Bioxcell® ile sulandırılmış grupta elde edilmiştir. Optidyl® sulandırıcısı kullanılan grupta, Bioxcell® sulandırıcısına kıyasla daha yüksek ilerleyen motilite oranı elde edilmiştir (p<0.01). Bioxcell® ve Tris sulandırıcısı kullanıldığında daha yüksek oranda akrozomal ve toplam anormallikler saptanmıştır. ALH yönünden, en yüksek değer diğer gruplarla karşılaştırıldığında Optidyl sulandırıcısından elde edilmiştir (p<0.05). Öneri: Holştayn ve İsviçre-Esmeri boğa spermasının dondurulması amacıyla Optidyl® sulandırıcısı Tris + yumurta sarısı ile Bioxcell® sulandırıcılarına tercih edilebilir.Öğe Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015) Sariozkan, Serpil; Bucak, Mustafa Numan; Tuncer, Purhan Barbaros; Buyukleblebici, Serhat; Eken, Ayse; Akay, CemalIt was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P > 0.05). Sperm acrosome and total abnormalities were similar in all groups (P > 0.05). Groups M (hyaluronan + fetuin) and H (hyaluronan) displayed a higher rate of sperm membrane integrity, compared to that of Group C (control) (P < 0.01). According to the results of the comet assay, the lowest percentage of sperm with damaged DNA was achieved in Group H, when compared to all of the experimental groups (P < 0.01). Furthermore, all of the additives resulted in a lower rate of sperm with damaged DNA than that of the controls, and thus, reduced DNA damage (P < 0.01). For pregnancy rates, there were no significant differences between the extender groups (P > 0.05). MDA formation was found to be lower in Groups M and F (P < 0.01). In Group M, SOD activity was determined to have significantly increased (23.61 +/- 5.62 U/ml) compared to the other groups (P < 0.01). All experimental groups had a GSH-Px activity higher than that of the control group (P < 0.01). Crown Copyright (C) 2015 Published by Elsevier Inc. All rights reserved.Öğe Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014) Sariozkan, Serpil; Bucak, Mustafa Numan; Tuncer, Purhan B.; Buyukleblebici, Serhat; Canturk, FazileSupplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 +/- 0.24% and 4.80 +/- 0.20%), glutamine (2.80 +/- 0.20% and 6.40 +/- 0.40%), carnitine (2.60 +/- 0.24% and 6.00 +/- 0.63%) and methionine (3.40 +/- 0.51% and 9.20 +/- 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 +/- 0.44 and 15.60 +/- 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 +/- 1.70% and 62.40 +/- 1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 +/- 4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2 mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17 +/- 0.18%) lower than those of the control group (3.16 +/- 0.32%) (P < 0.001). For pregnancy rates, there were no significant differences among the extender groups (P > 0.05). Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved
