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    The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4 degrees C
    (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012) Sariozkan, Serpil; Bucak, Mustafa Numan; Canturk, Fazile; Ozdamar, Sairn; Yay, Arzu; Tuncer, Purhan Barbaros; Ozcan, Servet
    This study evaluated the protective effects of supplementation with three different sugars on the motility, morphology and DNA integrity of rat epididymal sperm chilled and stored at 4 degrees C Epididymides were obtained from each donor. Rat epididymal sperm was diluted in Ham's F10 plus raffinose, Ham's F10 plus trehalose, Ham's F10 plus fructose, and Ham's F10 medium for control purposes. Thereafter, the extended sperm were chilled and stored in liquid form at 4 degrees C. Sperm motility, morphological abnormalities and DNA damage were determined at 0 and 12 h after chilling. No significant difference was observed in any of the parameters evaluated at 0 h, before storage (P > 0.05). After 12 h of storage, all sugar additives led to statistically higher motility, normal sperm morphology and DNA integrity in comparison to the control group. Raffinose gave the best motility percentages (32.86 +/- 1.84%) after 12 h of storage at 4 degrees C, compared to the other groups (P < 0.001). In conclusion, Raffinose, trehalose and fructose provided a better protection of sperm functional parameters against chilling injury, in comparison to the control group. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
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    Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability
    (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014) Sariozkan, Serpil; Bucak, Mustafa Numan; Tuncer, Purhan B.; Buyukleblebici, Serhat; Canturk, Fazile
    Supplementation of the semen extender with antioxidants did not produce any significant effect on CASA and progressive motilities and sperm motility characteristics, in comparison to the control group (P > 0.05). For sperm acrosome and total abnormalities, TCM-199 supplemented with cysteine (2.60 +/- 0.24% and 4.80 +/- 0.20%), glutamine (2.80 +/- 0.20% and 6.40 +/- 0.40%), carnitine (2.60 +/- 0.24% and 6.00 +/- 0.63%) and methionine (3.40 +/- 0.51% and 9.20 +/- 0.86%) at doses of 2 mM provided a better protective effect, compared to that of the controls (8.00 +/- 0.44 and 15.60 +/- 1.895). As regards sperm membrane integrity, supplementation with 2 mM of glutamine and methionine (56.00 +/- 1.70% and 62.40 +/- 1.78%, respectively) resulted in higher rates, when compared to the control group (41.40 +/- 4.74%). According to the results of the COMET assay, only the use of TCM-199 supplemented with 2 mM of cysteine reduced DNA damage and resulted in percentages of sperm with damaged DNA (2.17 +/- 0.18%) lower than those of the control group (3.16 +/- 0.32%) (P < 0.001). For pregnancy rates, there were no significant differences among the extender groups (P > 0.05). Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved