Yazar "Celik, I." seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Cytochemistry of peripheral blood leukocytes in thoroughbred foals(TAYLOR & FRANCIS INC, 2013) Ozaydin, T.; Celik, I.; Sur, E.; Oznurlu, Y.; Uluisik, D.We attempted to characterize the cytochemical staining patterns of leukocytes and to determine the percentages of alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (ACPase) positive lymphocytes in peripheral blood of thoroughbred foals at different ages. Blood samples were obtained from the jugular veins of 60 healthy thoroughbred foals, 1 day, 3 days and 1 year old. Each age group included 10 male and 10 female animals. Peroxidase (PO) activity was detected in neutrophils, eosinophils and monocytes. Lymphocytes were negative for PO staining. Periodic acid-Schiff (PAS) staining was observed in neutrophils and monocytes; eosinophils were negative. The majority of lymphocytes were negative, but a few cells showed granular PAS positivity. Monocytes were strongly positive for ANAE and ACPase, and the enzymatic reaction was common in lymphocytes. Neutrophils showed a weakly positive reaction for ANAE and ACPase. Eosinophil granules were negative or weakly positive for ANAE and usually negative for ACP, but intergranular areas were positive. Mean ANAE positive peripheral blood lymphocytes (PBL) were 67.70, 73.10 and 49.20% in females; 64.00, 70.53, and 50.60% in males 1 day, 3 days, and 1 year old, respectively. ACPase positive PBL were 27.33, 32.83, and 37.40% in females; 29.67, 31.67, and 38.40% in males 1 day, 3 days, and 1 year old, respectively. For both enzymes, the differences between mean values for the genders were not statistically significant, but significant differences were found with regard to age. We provide comparative hematological data as a guide for identifying blood cells in thoroughbred foals.Öğe Enzyme histochemical and serological investigations on the immune system from chickens treated in ovo with aflatoxin B-1 (AFB(1))(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2011) Sur, E.; Celik, I.; Oznurlu, Y.; Aydin, M. F.; Oguz, H.; Kurtoglu, V.; Ozaydin, T.In this study, the detrimental effects of in ova administrated antitoxin B-1 (AFB(1)) on the immune system of chickens in the post-hatching period were investigated by enzyme histochemical and serological methods. For this purpose, 730 laying hen eggs were divided into 7 groups [3 control groups (not-treated, drilled-sealed and 30% ethanol (solvent)-injected groups) and 4 assay groups in which eggs were injected with increasing AFB(1) doses (2.5, 7.5, 12.5 and 17.5 ng/egg)] then conventionally incubated. The chickens were vaccinated against the Newcastle Disease (ND) and the Infectious Bursal Disease (I BE)) viruses on days 2, 5, 20 and on days 10 and 15 post-hatching, respectively. The serum antibody titres were assayed at the 1(st) and 28(th) days using Haemagglutination-Inhibition Test and ELISA respectively. The proportions of the peripheral blood lymphocytes (PBL) and the ANAE (alphanaphtyl acetate esterase) or ACP-ase (acid phosphatase) positive lymphocytes/PBL ratios were determined on the days 1, 10,20 and 28. The AFB(1)-treatment caused significant decreases in both the ANAE and ACP-ase positivity ratios of PBL., depending on the AFB(1) dose, during the post-hatching period, but the PBL counts were significantly depressed at the end of the experimental period only in chickens treated in ovo by the highest AFB(1) close (17.5 ng/egg). The anti-IBD antibody titres measured on the hatching day (maternal antibodies) and the anti-ND and IBD antibody titres measured on the day 28 post-hatching were significantly reduced in chicks treated with 12.5 and 17.5 ng AFB(1)/egg compared to the controls and have also gradually declined according to the AFB(1) dose. The results clearly evidenced that AFB(1) transferred into the fertilised eggs has caused deficiency in cellular and Immoral immunity and particularly in the embryo transfer of the maternal antibodies, that can partially explain the observed immunosuppressive AFB(1) effects in poultry.Öğe Hematology and Enzyme Histochemistry of Peripheral Blood Lymphocytes in Domestic Pigeon (Columba Livia f. Domestica)(Informa Healthcare, 2012) Oznurlu, Y.; Sur, E.; Celik, I.; Ozaydin, T.The purpose of our study was to determine the percentages of alpha-naphthyl acetate esterase (ANAE)-positive and acid phosphatase (ACP)-positive peripheral blood lymphocytes (PBL) in both sexes of one-year-old domestic pigeons (Columba livia f. domestica). Blood samples obtained from 12 healthy domestic pigeons were used. The mean percentages of ANAE-positive PBL for females and males were 47.8% and 48.8%, respectively, whereas the mean percentages of ACP-ositive PBL were 67.5% and 68.6%, respectively. The proportions of PBL were 49.3% and 48.6% in males and females, respectively.Öğe Histological and Enzyme Histochemical Investigation of The Hemal Nodes of The Hair Goat(Informa Healthcare, 2012) Ozaydin, T.; Sur, E.; Celik, I.; Oznurlu, Y.; Aydin, M. F.We investigated the structure of the hemal node in six healthy hair goats using histological and enzyme histochemical methods. After processing, tissue sections were stained with Crossman's trichrome, Gordon-Sweet's silver and Pappenheim's panoptic stains. Alpha-naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) were demonstrated in frozen sections. Hemal nodes were encapsulated by connective tissue and few smooth muscle cells. Several trabeculae originated from the capsule and extended into the hemal node. A subcapsular sinus was present beneath the capsule and was continuous with the deeper sinuses. Subcapsular and deep sinuses were filled with erythrocytes. The parenchyma consisted of lymphoid follicles, diffuse interfollicular lymphocytes and irregular wide lymphoid cords. Cortical and medullary regions were not distinct. ANAE (+) and ACP-ase (+) cells were located mainly in the germinal centers of the lymphoid follicles and also were scattered equally in the interfollicular region and lymphoid cords. Monocytes, macrophages and reticular cells displayed a diffuse positive reaction, whereas localized granular positivity was observed in lymphocytes. We demonstrated that the general structure of the hair goat hemal nodes is similar to that of other ruminant species.Öğe Investigation of Alpha-Naphthyl Acetate Esterase and Acid Phosphatase in the Peripheral Blood Leukocytes of Greyhounds(Informal Healthcare, 2012) Aydin, M. F.; Celik, I.; Sur, E.The purpose of our study was to determine the percentages of alpha-naphthyl acetate esterase (ANAE)and acid phosphatase (ACP)-positive peripheral blood lymphocytes (PBL), the presence of the ANAE and ACP enzymes in leukocytes, and the proportion of PBL in greyhounds. Peripheral blood samples were collected from the cephalic antebrachial vein of 14 (7 animals of each sex) healthy 1-2-year-old greyhounds. Mean percentages of ANAE-positive PBL were found to be 73.29 +/- 0.95% in female and 74.29 +/- 2.21% in male dogs. The difference between mean values of the genders was not statistically significant. The ACP values were 36.00 +/- 2.94% for females and 33.57 +/- 2.15% for males. No significant differences were found with regard to gender. For both enzymes, although monocytes and eosinophilic granulocytes displayed a positive reaction, neutrophils gave negative reactions. The proportion of PBL was 36.29 +/- 5.31% and 33.00 +/- 2.38 % in female and male dogs, respectively. The differences were not significant.Öğe A New Object Coding System for Computer-Aided Process Planning Applications(SPRINGER HEIDELBERG, 2013) Celik, I.; Unuvar, A.Recently, using features to model a part has been thought to be a key factor for the integrated design and manufacturing. The features that build the part are modeled as manufacturing features. Thanks to object-based modeling, features are structured systematically. In this paper, a feature coding method is proposed for prismatic parts to be produced and the use of this method in process planning is explained. After been determined, features are structured systematically through object-oriented modeling. This article explains an object coding method developed for prismatic workpieces and its use in process planning. Features were determined and modeled as objects. Features were coded according to their types and locations on the workpiece in this given method. Feature codings have been seen to be very advantageous in process planning.
