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    Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis
    (AMER VETERINARY MEDICAL ASSOC, 2012) Kennedy, Sarah A.; Constable, Peter D.; Sen, Ismail; Couetil, Laurent
    Objective-To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples. Sample-Blood samples obtained from 8 healthy horses. Procedures-Heparinized jugular venous blood was equilibrated via a tonometer at 37 degrees C with 12% O-2 and 5% CO2. Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0 degrees C) or at room temperature (22 degrees C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis. Results-Blood Po-2 remained constant for the reference method (GS stored at 0 degrees C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22 degrees C. In contrast, Po-2 increased when blood was stored at 0 degrees C in a GPPS and PSBGA or at 22 degrees C in a GPPS; however, Po-2 did not change when blood was stored at 22 degrees C in a PSBGA. Calculated values for plasma concentration of HCO3 and total CO2 concentration remained constant in the 3 syringe types when blood was stored at 22 degrees C for 2 hours but increased when blood was stored in a GS or GPPS at 0 degrees C. Conclusions and Clinical Relevance-Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0 degrees C or collected into a PSBGA and stored at room temperature. (Am J Vet Res 2012;73:979-987)