Yazar "Dall'Acqua, Stefano" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim
    Öğe
    Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications
    (ELSEVIER SCIENCE BV, 2019) Sut, Stefania; Dall'Acqua, Stefano; Zengin, Gökhan; Şenkardeş, İsmail; Bulut, Gizem; Cvetanovic, Aleksandra; Stupar, Alena; Mandić, Anamarija; Picot-Allain, Carene; Doğan, Ahmet; Sinan, Kouadio Ibrahime; Mahomoodally, Fawzi
    The phytochemical composition of different extracts obtained from stinking chamomile (Anthemis cotula L) was investigated. Ethanol was used as solvent and accelerated solvent extraction (ASE), microwave assisted extraction (MAE), maceration, soxhlet extraction (SE), and ultrasound assisted extraction (UAE) were applied on plant material. Comparison of the phytochemical contents, antioxidant, and enzyme inhibitory properties were performed. The most abundant sesquiterpene in the extracts was anthecotuloide, while the most present phenolics were caffeoyl quinic acid, quercetin, and kaempferol derivatives. UAE extract was the most efficient in the extraction of sesquiterpenoids and polyphenols. Considering the assays on antioxidant activity and enzyme inhibition, ASE extract showed highest phenolic content (62.92 mg gallic acid equivalent/g extract). Likewise, this extract showed highest radical scavenging (103.44 mg trolox equivalent [TE]/g extract and 155.70 mg TE/g extract, for DPPH and ABTS assays respectively) and reducing power potential (435.32 and 317.89 mg TE/g extract, for CUPRAC and FRAP assays, respectively). The different extracts showed similar results in the enzyme inhibition assays suggesting that the extraction methods used have no effect on observed enzyme activities. Novelty of our findings are the inhibitory action of the ethanol extract of A. cotula aerial parts on key enzymes associated with Alzheimer's disease (acetyl cholinesterase, butyryl cholinesterase), type 2 diabetes (alpha-amylase, alpha-glucosidase), and skin hyperpigmentation disorders (tyrosinase). Data collected from the present work further appraises the multiple potential biological properties of stinking chamomile suggesting the need for further investigation on its constituents. (C) 2019 Elsevier B.V. All rights reserved.
  • Küçük Resim
    Öğe
    LC-MS, NMR fingerprint of Potentilla argentea and Potentilla recta extracts and their in vitro biopharmaceutical assessment
    (ELSEVIER SCIENCE BV, 2019) Sut, Stefania; Dall'Acqua, Stefano; Uysal, Şengül; Zengin, Gökhan; Aktümsek, Abdurrahman; Picot-Allain, Caren; Mahomoodally, Mohamad Fawzi
    Many Potentilla species are used as traditional drugs in several countries and also studied for their possible therapeutic properties. As part of our continuous effort to shed light on the biopharmaceutical potential of such plant species, this study was aimed to establish the phytochemical fingerprinting and to evaluate biopharmaceutical potential of methanol, ethyl acetate, and water extracts obtained from the aerial parts of Potentilla recta L. and P. argentea L Combination of 1 dimension, 2 dimension-nuclear magnetic resonance as well as LC-DAD-ESI-MS and LC-APCI-MS allowed the identification of ellagitannins, flavonol glycosides, and triterpene constituents. To quantify different constituents in the extracts obtained with different solvents, the total contents in phenolics, flavonoids, saponins, and triterpenoid were evaluated. The antioxidant activity was assessed by the means of metal chelation, phosphomolybdenum, radical quenching (by 2,2-dipheny1-1-picrylhydrazyl [DPPFI] and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)[ABTS]) and reduction ability (by cupric reducing antioxidant capacity [CUPRAC] and ferric reducing antioxidant power [FRAP]) assays. Phytochemical analysis showed similar composition of the tested methanol extracts. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, alpha-amylase, and alpha-glucosidase were selected as target enzymes to detect enzyme inhibitory potentials of these extracts. The water extract of P. argentea (113.29 mg gallic acid equivalent/g extract) contained the highest phenolic amount while the methanol extract (519.70 mg quillaja equivalent/g extract) showed the highest saponin content. The water extract of both Potentilla species was the most potent ARTS scavenger as well as present the highest CUPRAC activity. The most potent inhibitory activity against AChE and BChE was observed for the ethyl acetate extracts of P. recta and P. argentea. The methanol extracts of P. recta (53.16 mmol acarbose equivalent [ACAS]/g extract) and P. argentea (45.68 mmol ACAE/g extract) were the most active inhibitors of alpha-glucosidase. Data collected from this study support the multiple therapeutic actions of P. recta and P. argentea and justify further pharmacological investigations for potential applications in the pharmaceutical industry.
  • Küçük Resim
    Öğe
    Paeonia arietina and Paeonia kesrounansis bioactive constituents: NMR, LC-DAD-MS fingerprinting and in vitro assays
    (ELSEVIER SCIENCE BV, 2019) Sut, Stefania; Zengin, Gökhan; Dall'Acqua, Stefano; Gazdova, Marketa; Smejkal, Karel; Bulut, Gizem; Doğan, Ahmet; Haznedaroğlu, Mehmet Zeki; Aumeeruddy, Muhammad Zakariyyah; Maggi, Filippo; Mahomoodally, Mohamad Fawzi
    Paeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective effects. The multiple phytother-apeutical applications of Paeonia species inspired us to establish the phytochemical fingerprint and to evaluate the biological properties of ethyl acetate, methanol, and aqueous extracts from the roots and aerial parts of two Paeonia species (P. arietina G. Anderson and P. kesrounansis Thiebaut). Phytoconstituents of P. arietina and P. kesrounansis extracts were analyzed using 1D and 2D NMR and LC-DAD-ESI-MS. The total content of phenolics (TPC) and flavonoids (TFC) in the extracts was also evaluated. The antioxidant activity was profiled using DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation assays. Enzyme inhibitory properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, alpha-amylase, and alpha-glucosidase. Phytochemical analysis of P. arietina and P. kesrounansis extracts showed the presence of galloyl esters of sugars, galloyl monoterpenes, and glycosylated flavonoids. The three solvent extracts presented different behavior in the bioassays. The highest antioxidant activity, tyrosinase and AChE inhibition were observed for the methanolic extract of the aerial parts of P. kesrounansis. In addition, the ethyl acetate extracts of the aerial parts of both plants were the most effective inhibitors of alpha-amylase. The highest BChE inhibition was observed for root methanolic extract of P. kesrounansis while the root ethyl acetate extract of P. arietina exerted the strongest inhibition of alpha-glucosidase. Methanol extract of P. kesrounansis aerial parts presented the highest TPC, while TFC was greatest in the corresponding extract of P. arietina. Our findings can be considered as a starting point for future studies to further validate the effectiveness and safety profiles of these plants in folk medicine. (C) 2018 Elsevier B.V. All rights reserved.
  • Küçük Resim
    Öğe
    Phytochemical Fingerprinting and In Vitro Bioassays of the Ethnomedicinal Fern Tectaria coadunata (J. Smith) C. Christensen from Central Nepal
    (MDPI, 2019) Shrestha, Shyam Sharan; Sut, Stefania; Di Marco, Serena Barbon; Zengin, Gökhan; Gandin, Valentina; De Franco, Michele; Pant, Deepak Raj; Mahomoodally, Mohamad Fawzi; Dall'Acqua, Stefano; Rajbhandary, Sangeeta
    Tectaria coadunata, an ethnomedicinal fern used in Nepal to treat a large number of diseases, has been poorly studied with regard to its phytochemical composition and possible bioactivity. This study was performed with the aim of supporting traditional medicine as a new source of bioactive constituents. Phytochemical compositions of methanol extracts were determined by nuclear magnetic resonance (NMR), liquid chromatography-diode array detector-mass spectrophotometry (LC-DAD-MS), and liquid chromatography-fluorescence-mass spectrometry. Quali-quantitative data revealed large amount of procyanidins, mainly of the A-type, as well as eriodictyol-7-O-glucuronide and luteolin-7-O-glucoronide as main constituents. The antioxidant, cytotoxic, and inhibitory activity of five enzymes that are implicated in human diseases was evaluated for the extract and fractions. High free-radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays and inhibitory activities against cholinesterases and tyrosinase were observed. Furthermore, a moderate cytotoxic effect was observed on the 2008 and BxPC3 cell lines. Overall results showed potential usefulness of this fern as a source of phytochemicals for pharmaceutical uses.
  • Küçük Resim
    Öğe
    Ricinodendron heudelotii (Baill.) Heckel stem barks and seed extracts, a native food plant from Africa: Characterization by NMR and HPLC-DAD-ESI-MSn
    (ELSEVIER, 2020) Sut, Stefania; Dall'Acqua, Stefano; Bene, Kouadio; di Marco, Serena Barbon; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad Fawzi; Picot-Allain, Marie Carene Nancy; Zengin, Gökhan
    Ricinodendron heudelotii (Baill.) Heckle is used as food ingredient and in the African traditional medicine. In the present study inhibitory activity on alpha-amylase, alpha-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase of ethyl acetate, methanol, and water extracts of R. heudelotii seeds and stem bark were assessed. Stem bark extracts exhibited significant antioxidant properties. Ethyl acetate extract of seed had great inhibitory potential against alpha-glucosidase, acetylcholinesterase, and butyrylcholinesterase. Nuclear magnetic resonance (NMR) and high-performance liquid chromatography with electrospray ionization mass spectrometry (HPLCDAD-ESI-MSn) analysis revealed the presence of catechin and gallic acid derivatives in bark while fatty acid in seeds. Multivariate analysis of obtained data was performed showing a clear separation between seed and stem bark. Obtained results indicate R. heudelotii stem bark as new starting materials for the development of novel pharmaceutical formulations.
  • Küçük Resim
    Öğe
    Triterpene acid and phenolics from ancient apples of friuli venezia giulia as nutraceutical ingredients: lC-MS study and in vitro activities
    (MDPI, 2019) Sut, Stefania; Zengin, Gökhan; Maggi, Filippo; Malagoli, Mario; Dall'Acqua, Stefano
    Triterpene acid and phenolic constituents from nine ancient varieties of apple (Malus domestica) fruits cultivated in Fanna, Friuli Venezia Giulia region, northeast Italy, were analyzed and compared with four commercial apples (Golden Delicious', Red Delicious', Granny Smith' and Royal Gala'). Total phenolic and flavonoid contents were measured by spectrophotometric assays. The quali-quantitative fingerprint of secondary metabolites including triterpene acid was obtained by LC-DAD-(ESI)-MS and LC-(APCI)-MS, respectively. Based on the two LC-MS datasets, multivariate analysis was used to compare the composition of ancient fruit varieties with those of four commercial apples. Significant differences related mainly to the pattern of triterpene acids were found. Pomolic, euscaphyc, maslinic and ursolic acids are the most abundant triterpene in ancient varieties pulps and peels, while ursolic and oleanolic acids were prevalent in the commercial fruits. Also, the content of the phenolic compounds phloretin-2-O-xyloglucoside and quercetin-3-O-arabinoside was greater in ancient apple varieties. The antioxidant (radical scavenging, reducing power, metal chelating and phosphomolybdenum assays) and enzyme inhibitory effects (against cholinesterase, tyrosinase, amylase and glucosidase) of the samples were investigated in vitro. Antioxidant assays showed that the peels were more active than pulps. However, all the samples exhibited similar enzyme inhibitory effects. Ancient Friuli Venezia Giulia apple cultivars can be a source of chlorogenic acid and various triterpene acids, which are known for their potential anti-inflammatory activity and beneficial effects on lipid and glucose metabolism. Our results make these ancient varieties suitable for the development of new nutraceutical ingredients.