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    Attachment, proliferation and collagen type I mRNA expression of human gingival fibroblasts on different biodegradable membranes
    (TAYLOR & FRANCIS INC, 2013) Hakki, Sema S.; Korkusuz, Petek; Purali, Nuhan; Bozkurt, Buket; Kus, Mahmut; Duran, Ismet
    The purpose of this study was to investigate adhesion, proliferation and type I collagen (COL I) mRNA expression of gingival fibroblasts on different membranes used in periodontal applications. Collagen (C), acellular dermal matrix (ADM) and polylactic acid; polyglycolic acid; lactide/glycolide copolymer (PLGA) biodegradable membranes were combined with gingival fibroblasts in culture and incubated for 48 h. Cell adhesion was examined with scanning electron and confocal microscopy. MTT assay was used to measure proliferation. COL I mRNA expression was assessed using quantitative-polymerase chain reaction (QPCR). The PLGA group exhibited the lowest cell survival on day 5 and 10, and lowest cell proliferation on days 5, 10 and 14. While cell proliferation was similar in C and ADM groups, the C membrane showed a slightly greater increase in viable cells to day 10. Confocal and scanning electron microscopy confirmed the results of proliferation and MTT assays. The highest COL I mRNA expression was noted in the PLGA membrane group when compared to the C (p<0.01) and ADM (p<0.05) membrane groups. These data revealed that adherence and proliferation of primary gingival fibroblasts on collagen-based C and ADM membranes is better than that seen with PLGA membranes, and thus may be preferable in the treatment of gingival recession defects.
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    Efficacy of Collagen Membrane Seeded With Autologous Gingival Fibroblasts in Gingival Recession Treatment: A Randomized, Controlled Pilot Study
    (WILEY, 2013) Koseoglu, Serhat; Duran, Ismet; Saglam, Mehmet; Bozkurt, S. Buket; Kirtiloglu, Osman S.; Hakki, Sema S.
    Background: Gingival recession (GR) is one of the most common esthetic concerns associated with periodontal tissues. Recently, tissue engineering technology has been developed and applied in periodontology for the treatment of GR. The aim of this study is to compare the clinical efficacy of collagen membrane with or without autologous gingival fibroblasts under a coronally advanced flap for root coverage. Methods: In this split-mouth, controlled clinical study, 22 sites are selected from 11 patients with Miller Class I recessions affecting canines or premolars in the maxillary arch. One tooth in each patient was randomized to receive either a collagen membrane (CM) (control group) or a collagen membrane seeded with autologous gingival fibroblasts (CM+GF) (test group) under a coronally advanced flap. Thickness of the gingiva, GR, and percentage of root coverage (PRC) were recorded by a calibrated examiner at baseline and 3, 6, and 12 months postoperatively. Furthermore, GR and PRC were evaluated using photogrammetric analysis at baseline and 3, 6, and 12 months. Results: Both treatments resulted in a significant gain in root coverage compared with baseline. A statistically significant increase was detected in PRC in the test group compared with the control group. No significant difference was noted between the test and control sites regarding the thickness of the gingiva. Conclusions: The results indicated that CM+GF prepared by tissue engineering technology can be considered an alternative method for the treatment of Miller Class I recession defects.
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    Relationship Between Iron-Deficiency Anemia and Periodontal Status in Female Patients
    (AMER ACAD PERIODONTOLOGY, 2009) Enhos, Sukru; Duran, Ismet; Erdem, Sami; Buyukbas, Sadik
    Background: The aim of this study was to evaluate the periodontal status of patients with iron-deficiency anemia (IDA) and the correlation of changes in serum and gingival crevicular fluid (GCF) ferritin levels after periodontal therapy. Methods: Nineteen female patients with anemic hematologic values were classified as group A, and 20 healthy females with normal hematologic values were classified as group B. After group A was recruited, group B was enrolled with patients who had similar gingival indices as group A. At baseline and the 3-month follow-up visit, clinical periodontal indices and hematologic parameters were recorded, and GCF samples were taken. All patients received an oral hygiene-improvement session followed by scaling, and sites with >4-mm probing depths received root planing. At the 3-month follow-up visit, all measurements and analyses were repeated. Results: During the follow-up period, all clinical indices decreased in both groups (P<0.05), but the gingival index in group A did not change. The GCF ferritin concentration showed statistically significant decreases (P<0.05), but total amounts of ferritin in GCF did not change. No significant correlation was found between serum and GCF ferritin levels. Conclusion: The findings of this study showed that changes in serum ferritin levels did not correlate with the GCF ferritin levels, and IDA was not a direct risk factor for periodontal diseases. J Periodontol 2009;80:1750-1755.