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    The effect of buserelin injection 12 days after insemination on selected reproductive characteristics in cows
    (VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2011) Ataman, Mehmet B.; Erdem, Huseyin; Bulbul, Bulent; Umutlu, Seyit; Colak, Mehmet
    The aim of this study was to evaluate the effect of buserelin injection on day 12 postinsemination on fertility in lactating dairy cattle. A total of 57 cows were assigned to two groups and four subgroups. In the treatment group, the cows were synchronized with PGF(2 alpha)-PGF(2 alpha) (group A) or GnRH-PGF(2 alpha) (group B) protocol, and buserelin was injected on day 12 postinsemination. Cows in the control group were synchronized with PGF(2 alpha)-PGF(2 alpha) (group C) or GnRH-PGF(2 alpha) (group D) protocol, saline solution was injected on day 12, and served as controls. Pregnancy rates on day 21 and 45 and embryonic death rates were 85.7%, 71.4% and 16.7%, 85.7%, 85.7% and 0.0%, 73.3%, 62.1% and 27.3% and 85.7%, 71.4% and 16.7% in groups A, B, C and D, respectively. There was no significant difference between synchronization protocols for pregnancy rates, and among groups A, B, C and D for pregnancy rates and embryonic death rates. Mean progesterone concentrations in pregnant cows in groups A and B were higher than that in groups C and D, respectively, on day 18 and 21 (p < 0.05). In conclusion, GnRH injection on day 12 postinsemination increased the plasma progesterone concentrations on day 18 and 21 postinsemination. However, it did not alter the pregnancy rates and prevent embryonic deaths.
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    Effect of presence of corpus luteum at the beginning of ovsynch protocol on pregnancy rates in lactating dairy cows
    (KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2012) Cinar, Mahmut; Guzeloglu, Aydin; Erdem, Huseyin
    The aim of the study was to investigate the effect of presence of CL at the beginning of Ovsynch protocol on pregnancy rates in lactating dairy cows. A total of 218 lactating Holstein cows that were at least 45 days postpartum with no reproductive disorders were used in the present study. Cows were divided into two experimental groups according to the presence and absence of corpus luteum (CL) on the first day of transrectal ultrasonography (day 0). Cows having CL (CL (+)) were randomly allocated to two subgroups as CL (+)/A and CL (+)/B. Cows in CL (+)/A group (n = 74) were treated with Ovsynch protocol, while cows in CL (+)/B group (n = 78) were treated with PGF(2 alpha) on the first day of examination and seven days later Ovsynch protocol was started. Cows having no CL (CL (-)) (n = 66) were left untreated on the first day of examination and Ovsynch protocol was started seven days later. Pregnancy rates were found 23%, 25.6% and 40.9% in CL (+)/A, CL (+)/B and CL (-) groups, respectively. The pregnancy rate in CL (-) group was significantly higher than those of CL (+)/A, and CL (+)/B groups (P<0.05). It is suggested that the success of Ovsynch synchronization protocol may be related to starting time of the protocol and to ovarian physiology in cyclic dairy cows.