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Öğe The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey(TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2011) Erisen, Semiha; Atalay, Emine; Yorgancilar, MustafaThidiazuron (TDZ) is a cytokinin-like substance that has often been used for shoot regeneration in recent years. We observed the effect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with alpha-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Significant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. The highest number of shoots, (15 per explant) was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). The rooted plantlets were acclimatised and produced normal plants.Öğe HIGH FREQUENCY REGENERATION OF CRITICALLY ENDANGERED ENDEMIC PLANT CENTAUREA LYCAONICA(PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2017) Atalay, Emine; Erisen, SemihaCentaurea lycaonica is a critically endangered endemic plant species found in Turkey. In this study in vitro culture techniques were evaluated as an ex situ strategy for conservation of this species. Achenes were surface sterilized and cultured either in MS medium alone or along with GA(3) in an attempt to induce germination. It was observed that the overall germination rate was low and that the highest rate (20%) was obtained when hormone-free MS medium was used. For axillary shoot pro-pagation, nodal explants were cultured on MS me-dium supplemented with BAP, Kin or TDZ. The highest shoot number (8.7 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L BAP. Leaf explants were removed from the in vitro axillary shoots that were used as explants and cultured on medium including BAP, Kin, TDZ alone or with NAA. The calli obtained exhibited differences in morphology which appeared to be dependent on type and combination of PGR used. It was found that BAP was the only PGR that was capable of eliciting a positive response with respect to shoot regeneration. The highest shoot number (32.3 shoots per explant) was obtained on MS medium supplemented with 0.5 mg L-1 BAP and 0.2 mg L-1 NAA. Regenerated shoots were transferred into various rooting media. The best rooting rate (90%) was obtained on MS medium supplemented with 2 mg L-I IAA. Rooted shoots were successfully acclimatized and were observed to grow vigorously under ex vitro conditions.Öğe In vitro shoot multiplication of Anagyris foetida L.(CURRENT BIOLOGY LTD, 2011) Erisen, Semiha; Atalay, Emine; Yorgancilar, Mustafa; Oncel, Zeynep[Abstract not Available]Öğe Micropropagation of Stevia rebaudiana Bertoni(CURRENT BIOLOGY LTD, 2011) Atalay, Emine; Erisen, Semiha; Yorgancilar, Mustafa; Tanur, Munure[Abstract not Available]
