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    A Comparison of Immuncapture Agglutination and ELISA Methods in Sero-logical Diagnosis of Brucellosis
    (IVYSPRING INT PUBL, 2011) Ozdemir, Mehmet; Feyzioglu, Bahadir; Kurtoglu, Muhammed Guzel; Dogan, Metin; Dagi, Hatice Turk; Yuksekkaya, Serife; Kesli, Recep
    Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and im-muncapture agglutination tests with Coombs anti-brucella test. Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were in-cluded into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Im-muncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test. Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.
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    Investigation of microbial colonization of computer keyboards used inside and outside hospital environments
    (ANKARA MICROBIOLOGY SOC, 2008) Dogan, Metin; Feyzioglu, Bahadir; Oezdemir, Mehmet; Baysal, Buelent
    Computers have been commonly used in daily life and at hospitals by medical staff. This study was carried, out to search the microbial colonization of computer keyboards and mice used inside and outside hospital environments. Keyboards and mice samples from a total of 398 computers were included to the study, in which 38 were used by doctors and nurses in the hospital clinics (Group 1); 32 by the medical faculty students (Group 2), and 328 by university students (Group 3) in the computer laboratories of Selcuk University, Konya (located at middle Anatolia). Of the computers, 96.7% (n:385) have been found to be colonized by coagulase-negative staphylococci (CoNS), 13.1% (n:52)by gram-positive spore-forming bacilli and 8.8% (n:35) by corynebacteria; followed by Candida spp. (4.2%), gram-negative bacilli (1.7%) [Acinetobacter spp. (n:4), Pseudomonas sp. (n:1), Klebsiella sp. (n:1), E.coli (n:1)], Staphylococcus aureus (1.5%), and molds (Penicillium, Aspergillus; 1.2%). The isolation rates of CoNS were similar between the groups (94,7%, 93.7%, and 97.2%, respectively). However it was noted that all, of the gram-negative bacterial isolates (7/38; 18.4%) were from the samples collected from hospital computers (Group 1). Susceptibility rates of CoNS isolates to cefoxitin were detected as 26.2% in Group 1, 79.2% in Group 2, and 91.3% in Group 3. Five out of six S.aureus strains were found susceptible to cefoxitin, except one isolated from a sample of Group 1. Linezolid resistance in both CoNS and S.aureus isolates were not determined in any groups. As a result, according to the data obtained from this study as well as from the other foreign studies, the computer keyboards and mice which are widely used in the hospital settings, are being the source of potential cross contamination in the development of nosocomial infections. Therefore the computers should be cleaned properly frequently and hand washing procedures and disinfection rules should be obeyed after the use of computers before handling the patients.
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    Laboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodies
    (GEORG THIEME VERLAG KG, 2018) Feyzioglu, Bahadir; Yavru, Sibel; Ozdemir, Mehmet
    Introduction Central nervous system (CNS) viral infections are a serious problem requiring accurate diagnosis and treatment. Human herpesviruses (HHVs) are an important cause of these infections. Recent research has focused on new diagnostic methods allowing accurate and rapid identification of viral infections because there are still diagnostic difficulties for these infections. This study was done to determine the etiologic role of human herpes viruses and to obtain information that will contribute to the diagnostic algorithm in suspected cases of viral encephalitis or aseptic meningitis. Materials and Methods In our study, herpes simplex virus (HSV)-1, HSV-2, varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and HHV-6 DNA was detected in the cerebrospinal fluid (CSF) by multiplex polymerase chain reaction (PCR) and virus-specific immunoglobulin G (IgG) antibodies in CSF and serum by EIA in pediatric encephalitis/meningitis cases. Results HSV-1 and VZV were detected in 5 and 3.3% of aseptic meningitis cases, respectively, but no encephalitis cases. Other viruses were not identified as etiologic agents. The seroprevalences were determined as 72.4, 34.3, 81.9, 93.3, 88.6, and 80.9%, respectively for HSV-1, HSV-2, VZV, CMV, EBV, and HHV-6. The performance of specific IgG CSF/serum antibody index (AI) was not satisfactory. Conclusion Our study indicates that the multiplex PCR method is the most suitable for the diagnosis of CNS infections caused by HHVs. However, due to the high cost of the PCR method, the positive results of the specific AI may be significant, but the negative results are unreliable, especially in limited health care facilities.
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    A Rare Cause of Bacteremia in a Pediatric Patient with Down Syndrome: Sphingomonas Paucimobilis
    (IVYSPRING INT PUBL, 2011) Ozdemir, Mehmet; Pekcan, Sevgi; Demircili, Mehmet Emin; Tasbent, Fatma Esenkaya; Feyzioglu, Bahadir; Pirinc, Serife; Baykan, Mahmut
    Sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non fermentative, gram negative motile bacillus. S. paucimobilis which is widely found in nature and hospital environments rarely cause serious or life threatening infections. In this report, a case of hospital acquired bloodstream infection due to S. paucimobilis in a patient with Down syndrome who was on treatment for presumed pneumonia is presented. A one year-old child patient who was a known case of Down syndrome and had previously experienced cardiac surgery was hospitalized and treated for pneumonia. On the 12th day of hospitalization, blood cultures were taken because of a high body temperature. One of the blood cultures was positive for gram-negative rods. After 48 hour of incubation, the sub-cultures on blood agar medium yielded pure growth of a yellow, non-fermentative, gram-negative, rod-shaped bacterium. The microorganism was positive for oxidase, and esculin hydrolysis, while negative for urea and nitrate reduction, citrate utilisation and motility. The isolate had been identified as S. paucimobilis by using Vitek 2 system. The antibiotic susceptibility test was also performed with the same system and the strain was found to be susceptible to piperacillin-tazobactam and other antibiotics. Treatment with intravenous piperacilin-tazobactam (150 mg/kg/day) was initiated. He responded well to the treatment and was discharged after 10 days. This case is reported to emphasize that S. paucimobilis should be kept in mind as a nosocomial infectious agent in patients with Down syndrome and immunosuppressive patients and the infections should be treated according to the sensitivity test results.