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Öğe Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test(TAYLOR & FRANCIS LTD, 2016) Akın, Duygu; Durak, Yusuf; Uysal, Ahmet; Güneş, Erdoğan; Aladağ, Mustafa OnurContext: Celtis glabrata is used in Turkey for the treatment of various health disorders. Objective: The acetone, chloroform, ethanol, and methanol extracts of C. glabrata leaf, fruit, and seed were investigated to evaluate their antimutagenic activities. Material and methods: The antimutagenicity of these extracts was determined by Ames test against mutagens (4-nitro-O-phenylenediamine, 2-aminofluorene (2-AF), and sodium azide (SA)). The extracts were used at concentrations between 5 and 0.005 mg/plate. Results: The ethanol extracts of leaves exhibited strong antimutagenicity (70%) against 2-AF with S9 at 5 mg/plate on TA98. But methanol (61%, 53%) and acetone (53%, 52%) also revealed strong inhibition rates at concentrations of >= 0.5 mg/plate. Among the extracts, the highest activity (96%) was obtained from acetone extract against SA without S9, followed by chloroform extract (91%) at a dose of 5 mg/plate on TA100 with S9. Ethanol (without S9) and chloroform (with S9) extracts showed strong antimutagenicity at all doses. Exception of chloroform and acetone (without S9), all fruit extracts (with/without S9) manifested strong antimutagenicity at doses of >= 0.5 mg/plate on TA98 strain. Ethanol extracts revealed 68% inhibition against 2-AF on TA98. Acetone and ethanol extracts manifested 84% and 82% inhibition against SA on TA100, respectively. All the extracts of seeds revealed strong inhibition against 2-AF at >= 0.5 mg/plate doses on TA98, but acetone extract showed excellent antimutagenicity (94%). Moreover, the chloroform (74, 73, 63, 54%), acetone (74, 72, 70, 65%) and methanol (74, 67, 63, 61%) extracts of seeds revealed strong antimutagenic activity on TA100 against SA with S9. Discussion and conclusion: This plant may be natural source of antimutagenic agents.Öğe Biological and chemical insights of Morina persica L.: A source of bioactive compounds with multifunctional properties(ELSEVIER, 2016) Mocan, Andrei; Zengin, Gökhan; Uysal, Ahmet; Güneş, Erdoğan; Mollica, Adriano; Değirmenci, Nurdan Sena; Alpsoy, LokmanMorina persica L. has weak rosy smell flowers that together with aerial parts are infused or decocted and used as a functional tea. It is a representative of the Anatolian traditional medicine and is used for the treatment of cold. Biological and chemical fingerprints of different extracts (methanolic, acetone and water) of M. persica were investigated. Antioxidant, enzyme inhibitory, antimicrobial, cytotoxic and mutagenic/antimutagenic effects were evaluated for biological profiling. Rutin, chlorogenic and rosmarinic acids were detected as dominant compounds. Generally, the methanolic and acetone extracts had strong antioxidant, enzyme inhibitory, antimicrobial and antimutagenic effects. Additionally, all extracts showed remarkable inhibitory effects on HeLa cells at 100 mu g/mL. Furthermore, possible interactions between rutin, the dominant phenolic compound in M. persica and the tested enzymes were assessed by molecular docking. Results indicate that M. persica could be considered as a natural source of high-valued functional ingredients for further use in healthful formulations. (C) 2016 Elsevier Ltd. All rights reserved.Öğe Çerçeve kayması ve baz çifti değişimi mutasyonlarına karşı etkili doğal iki Taraxacum türü: Mutajenik, antimutajenik, antioksidan değerlendirme(MARMARA UNIV, FAC MEDICINE, 2016) Uysal, Ahmet; Zengin, Gökhan; Güneş, Erdoğan; Uysal, Nazife EkşinarSon yıllarda bitkiler ve bitkisel ürünler, düşük toksisite ve yan etkilerinden dolayı farmakoloji ve gıda endüstrilerinde artan bir önem kazanmışlardır. Asteraceae familyasına ait olan Taraxacum cinsi,eski çağlardan buyana geleneksel tıpta kullanılmıştır. Bu çalışmada iki Taraxacum türünün (Taraxacum mirabile ve Taraxacum farinosum) antioksidan, mutajenik ve antimutajenik özellikleri araştırıldı. Antioksidan özellikler; DPPH radikal süpürme, indirgeme gücü (CUPRAC ve FRAP) ve fosfomolibdenyum yöntemlerini içeren farklı test sistemleri ile araştırıldı. Aynı zamanda toplam fenolik ve toplam flavonoit içerikleri de bildirildi. Mutajenik ve antimutajenik aktiviteler Ames yöntemi ile test edildi. Taraxacum türlerinin orta düzeyde antioksidan ve ortadan güçlüye değişen oranlarda antimutajenik aktiviteye sahip olduğu görüldü. Buna rağmen türler mutajenik bulunmadı. T. mirabile’nin toplam fenolik ve flavonoit içerikleri (23.43 mgGAE/g ekstre ve 4.58 mgRE/g ekstre) T. farinosum’dan (17.54 mgGAE/g ekstre ve 3.37 mgRE/g ekstre) daha yüksek olduğu belirlendi.Öğe Çeşitli kaynaklardan izole edilen Staphylococcus aureus suşlarının metisilin direncinin farklı yöntemlerle araştırılması(Selçuk Üniversitesi Fen Bilimleri Enstitüsü, 2011-07-15) Güneş, Erdoğan; Durak, YusufBu çalışmada, çeşitli kaynaklardan izole ve identifiye edilen 150 Staphylococcus aureus bakteri suşunun metisilin direnci üç farklı yöntemle incelendi. Bu incelemede; agar tarama, disk difüzyon ve sıvı mikrodilüsyon yöntemleri kullanıldı. Agar tarama ve mikrodilüsyon yönteminde 16 ( % 10.7) suş, disk difüzyon yönteminde ise 17 ( % 11.3) suş Methicillin Resistance Staphylococcus aureus (MRSA) olarak saptandı. Agar tarama yöntemi referans alınarak diğer iki yöntemin duyarlılık ve özgüllükleri belirlendi. Agar tarama ve mikrodilüsyon yöntemi birbirlerine % 100 uyumlu bulundu. Disk difüzyon yönteminin duyarlılığı % 100, özgüllüğü ise % 99.2 olarak bulundu. Agar tarama yöntemi, diğer yöntemlere göre daha pratik görüldüğünden, metisilin direncini belirlemede önerilebileceği sonucuna varıldı. Clinical and Laboratory Standards Institute (CLSI) önerileri doğrultusunda, disk difüzyon yöntemiyle tüm suşların çoklu antibiyotik direnci de araştırıldı. İncelenen MRSA ve MSSA suşlarında sırasıyla; penisiline % 100 ve % 93.3, tetrasikline % 81.2 ve % 5.2, , ofloksasine % 62.5 ve % 1.5, rifampine % 56.2 ve % 0.7, amoksisilin/klavulanik aside % 56.2 ve % 0, eritromisine % 50 ve % 5.9, gentamisine % 50 ve % 0, trimetoprim sulfametoksazole % 18.7 ve % 0 oranlarında direnç saptanırken, tüm suşlar vankomisin ve linezolide duyarlı bulundu. MRSA suşlarındaki çoklu antibiyotik direncinin, MSSA suşlarına göre daha yüksek olduğu görüldü. Linezolidin MRSA enfeksiyonlarının tedavisinde vankomisine alternatif olabileceği kanısına varıldı.Öğe Characterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract infections: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotyping(POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2013) Aladağ, Mustafa Onur; Uysal, Ahmet; Dündar, Niyazi; Durak, Yusuf; Güneş, ErdoğanIn this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%),the resistance rates of non ESBL producing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.Öğe Chemical and biological insights on Cotoneaster integerrimus: A new (-)- epicatechin source for food and medicinal applications(ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2016) Uysal, Ahmet; Zengin, Gökhan; Mollica, Adriano; Güneş, Erdoğan; Locatelli, Marcello; Yılmaz, Turgut; Aktümsek, AbdurrahmanBackground: The Cotoneaster species are widely used as traditional purposes in different countries including Turkey. Purpose: The study was performed to evaluate the biological and chemical profile of two extracts (methanol (T-Me; F-Me) and water (T-W; F-W)) from two parts (twigs and fruits) of Cotoneaster integerrimus. Materials and methods: Antioxidant (free radical scavenging (DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating), enzyme inhibitory (cholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase), antimicrobial (standard microorganisms and methicillin-resistant Staphylococcus aureus isolates (MRSA)) and mutagenic/antimutagenic effects (by Ames assay) were tested for biological profile. For chemical profile, total and individual phenolic components were detected for each extract. Results: Generally, T-Me reflected the strongest biological effects with the highest level of phenolics (115. 15 mgGAEs/g extract). Also, twig extracts had more potent biological effects as compared to flower extracts. Eight-teen phenolics were identified in the extracts. (-)- epicatechin was the major constituent in all extracts and is mainly responsible for biological activities observed. Its amount present in F-W and T-W were 9.27 and 32.89 mg/g extract, respectively. Also, molecular docking was used to understand enzyme-epicatechin interactions. Conclusion: From these results, this plant has a great potential as a health promoter for developing novel functional food ingredients and pharmaceutical preparations. (C) 2016 Elsevier GmbH. All rights reserved.Öğe Genetic Diversity of Escherichia Coli Strains Isolated From Various Foods(ELSEVIER SCIENCE BV, 2012) Uysal, Ahmet; Durak, Yusuf; Güneş, Erdoğan; Aladağ, Mustafa Onur[Abstract not Available]Öğe Hyoscyamus Reticulatus’un Hekzan Ve Su Özütleri?ni?n Anti?oksi?dan Ve Anti?mi?krobi?yal Özelli?kleri? Üzeri?ne Bi?r Çalışma(Selçuk Üniversitesi Fen Fakültesi, 2014) Güneş, Erdoğan; Zengin, Gökhan; Uysal, Ahmet; Aktümsek, Abdurrahman; Durak, YusufBu çalışmanın amacı Hyoscyamus reticulatus’dan elde edilen hekzan ve su özütlerinin antioksidan kapasitelerini ve antimikrobiyal etkilerini belirlemektir. Antioksidan kapasite radikal süpürme (DPPH testi), toplam antioksidan kapasite, demir ve bakır indirgeme testlerini içeren dört farklı test sistemi ile araştırıldı. Antimikrobiyal etki sıvı mikro dilüsyon metodu ile değerlendirildi. Fosfomolibdat testi (total antioksidan kapasite) dışında, su özütünün hekzan özütünden daha yüksek antioksidan aktiviteye sahip olduğu belirlenmiştir. Toplam fenolik miktarı hekzan ve su özütünde sırasıyla 15.86 mg GAE/g ve 24.25 mg GAE/g olarak bulunmuştur. Ek olarak, hekzan özütü su özütüne kıyasla önemli bir antimikrobiyal etki göstermiştir. Bu çalışma ile elde edilen sonuçlar açıkça Hyoscyamus reticulatus’un oksidatif ve enfeksiyöz süreçlerde, doğal ajanların bir kaynağı olarak önemli bir potansiyele sahip olduğunu göstermiştir.Öğe New Perspectives on Asphodeline lutea from Bulgaria and Turkey: Anti-mutagenic, Anti-microbial and Anti-methicillin Resistant Staphylococcus aureus (MRSA) Activity(SCIENCEDOMAIN INT, 2016) Uysal, Ahmet; Lazarova, Irina; Zengin, Gökhan; Güneş, Erdoğan; Aktümsek, Abdurrahman; Gevrenova, RenetaAims: In this study, methanol extracts of Asphodeline lutea roots from Bulgaria (ALB) and Turkish (ALT) origin were evaluated for their anti-microbial, anti-MRSA properties and they were also screened for the potential of mutagenic and anti-mutagenic activities. Methodology: The broth micro dilution method was performed for the anti-microbial activities. For mutagenicity and anti-mutagenicity screening of the extracts, plate incorporation method of Ames test was employed. Results: Sarcina lutea was the most sensitive bacterium against ALB and ALT extracts at doses of 1.56 and 0.78 mg/ml, respectively. Both extracts exhibited similar activity against methicillin sensitive Staphylococcus aureus (MSSA) and Salmonella enteritidis with MIC value 6.25 mg/ml. Based on the results obtained from Ames test, no mutagenic activity was found for frame shift mutation (TA98) and base pair substitution (TA100) in all concentrations of A. lutea. Strong antimutagenic properties with rates of 77% and 75% were observed at the highest concentrations of both extracts against 2-aminofluorene-induced mutagenicity on TA 98 with the presence of metabolic activator S9 system. Conclusion: As a result, the extracts revealed significant anti-MRSA activity with MIC values 6.25 mg/ml against MRSA strains isolated from infections and manifested strong anti-mutagenic activity against known mutagens; it may be used in drug formulations against MRSA infections and may be used as a natural anti-mutagenic agent in the pharmacology and food industries.Öğe Selçuk Üniversitesi kampüsünden toplanan suillus bovinus (l.) roussel mantarının etanol ve metanol özütlerinin antimikrobiyal aktivitesi(Selçuk Üniversitesi, 01.10.2024) Güneş, ErdoğanBu çalışmanın amacı Suillus bovinus mantarının etanol ve metanol özütlerinin bazı standart patojen mikoorganizmalara karşı antimikrobiyal aktivitesini belirlemektir. Antimikrobiyal aktiviteyi saptamak için sıvı mikrodilüsyon metodu kullanılmıştır. S. bovinus'un metanol ve etanol özütünün, gram pozitif Staphylococcus aureus ve Sarcina lutea, gram negatif Salmonella enteritidis bakterilerine karşı 6.25 mg/ml dozda antibakteriyel aktivite gösterdiği tespit edilmiştir. Her iki özütün gram negatif Pseudomonas aeruginosa’ya ise 3.12 mg/ml dozunda antibakteriyal etki gösterdiği saptanmıştır. Escherichia coli ve Klebsiella pneumoniae bakterilerine karşı her iki özütün de etkisiz olduğu belirlenmiştir. Metanol ve etanol özütü sırasıyla 0.19 mg/ml ve 0.78 mg/ml MİK (Minimum İnhibisyon Konsantrasyonu) değeriyle en yüksek aktivite gösterdikleri suş ise gram pozitif Bacillu cereus suşu olmuştur. Ayrıca yapılan çalışmada Candida albicans maya suşuna karşı her iki özütün antifungal bir etki göstermediği görülmüştür. Sonuç olarak çalışmada, S. bovinus mantarının antibakteriyal aktiviteye sahip olduğu ve ileriki çalışmalarla bu türden elde edilen antimikrobiyal bileşiklerin, yeni ilaçların ve terapötik ajanların geliştirilmesi için potansiyel bir kaynak olacağı düşünülmektedir.Öğe Survey of Phytochemical Composition and Biological Effects of Three Extracts from a Wild Plant (Cotoneaster nummularia Fisch et Mey.): A Potential Source for Functional Food Ingredients and Drug Formulations(PUBLIC LIBRARY SCIENCE, 2014) Zengin, Gökhan; Uysal, Ahmet; Güneş, Erdoğan; Aktümsek, AbdurrahmanThis study was focused on the analysis of the phenolic content, antioxidant, antibacterial, anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase activity of three solvent extracts from Cotoneaster nummularia. Moreover, water extract was tested in terms of mutagenic/anti-mutagenic effects. The antioxidant activities of these extracts were evaluated by DPPH, ABTS, O-2, metal chelating, phosphomolybdenum, beta-carotene/linoleic acid, ferric and cupric reducing power assays. Enzyme inhibitory activities were also examined with colorimetric methods. Generally, methanol and water extracts exhibited excellent biological activities. These extracts were rich in phenolic and flavonoid content. Furthermore, Cotoneaster extracts indicated appreciable antibacterial properties against human pathogen strains. HPLC analysis showed that ferulic acid, chlorogenic acid, (-) - epicatechin and (+)-catechin were the major phenolics in extracts tested. These data offer that these extracts from C. nummularia may be considered as a potential source of biological agents for developing functional foods or drug formulations.Öğe Two Ganoderma species: profiling of phenolic compounds by HPLC-DAD, antioxidant, antimicrobial and inhibitory activities on key enzymes linked to diabetes mellitus, Alzheimer's disease and skin disorders(ROYAL SOC CHEMISTRY, 2015) Zengin, Gökhan; Sarıkürkcü, Cengiz; Güneş, Erdoğan; Uysal, Ahmet; Ceylan, Ramazan; Uysal, Şengül; Güngör, HalilThis work reports the antioxidant, antimicrobial, and inhibitory effects of methanol and water extracts from Ganoderma applanatum (GAM: methanol extract and GAW: water extract) and G. resinaceum (GRM: methanol extract and GRW: water extract) against cholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase. The total phenolics, flavonoids contents, and HPLC profile of phenolic components present in the extracts, were also determined. Antioxidant activities were investigated by using different assays, including DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum and metal chelating assays. Antimicrobial activity of the tested Ganoderma extracts was also studied by the broth microdilution method. Generally, the highest antioxidant (59.24 mg TEs per g extract for DPPH, 41.32 mg TEs per g extract for ABTS, 41.35 mg TEs per g extract for CUPRAC, 49.68 mg TEs per g extract for FRAP, 130.57 mg AAEs per g extract for phosphomolybdenum and 26.92 mg EDTAEs per g extract) and enzyme inhibitory effects (1.47 mg GALAEs per g extract for AChE, 1.51 mg GALAEs per g extract for BChE, 13.40 mg KAEs per g extract for tyrosinase, 1.13 mmol ACEs per g extract for alpha-amylase and 2.20 mmol ACEs per g extract for alpha-glucosidase) were observed in GRM, which had the highest concentrations of phenolics (37.32 mg GAEs g(-1) extract). Again, Ganoderma extracts possess weak antibacterial and antifungal activities. Apigenin and protocatechuic acid were determined as the main components in GRM (1761 mu g per g extract) and GAM (165 mu g per g extract), respectively. The results suggest that the Ganoderma species may be considered as a candidate for preparing new food supplements and can represent a good model for the development of new drug formulations.
