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Öğe Basal medium eagle solution may improve the post-thaw parameters of kangal dog semen(2016) Gungor, Sukru; Bucak, Mustafa NumanAmaç: Bu çalışmada dondurma-çözdürme sonrası Kangal köpeği spermatolojik parametreleri üzerine BME'nin etkisi-nin ortaya konulması amaçlandı.Gereç ve Yöntem: Ejakülatlar hafta iki kez digital maniplas-yon yardımıyla alındı. Alınan ejakülatlar 6 eşit hacme bölü-nerek temel Tris sulandırıcısı içeren (T), %5 gliserol (TG), %5 etilen glikol (TE), %5 gliserol (G) %2.5 BME (TGB2.5), TG %10 BME (TGB10), %5 etilen glikol (E) %2.5 BME (TEB2.5) ve TE %10 BME (TEB10) sulandırıcılar ile su-landırıldı. Sulandırılan spermalar 4C'ta 1.5 saat ekilibras-yon sonrası sıvı azot buharında dondurularak sıvı azotta (-196C) saklandı. Bulgular: Çalışmada progresif motilite oranı %10 BME içe-ren gliserollü sulandırıcı grubunda (%23.19), %5 etilen gli-kol içeren (%14.08) grubuna göre istatistiksel olarak yüksek bulundu. Akrozom bütünlüğü %2.5 BME içeren gliserollü sulandırıcı (%44.99) grubunda, %5 gliserol içeren (%33.63) grubuna göre istatiksel olarak üstünlük gösterdi (P0.05).Öneri: Kangal köpeği spermasının dondurulmasında sulan-dırıcıya eklenen BME çözüm sonu spermatolojik parametre-leri iyileştirebilir.Öğe Basal medium eagle solution may improve the post-thaw parameters of Kangal dog semen(Selçuk Üniversitesi Veterinerlik Fakültesi, 2016) Gungor, SukruAim: The aim of this study was to investigate the effects of BME on Kangal dog sperm parameters following the freezethawing process. Materials and Methods: Ejaculates were collected by digital manipulation twice a week. Ejaculates divided into six equal aliquots, and diluted with Tris-based extender (T) containing 5% glycerol (G) + BME 2.5% (TGB2.5), TG + BME 10% (TGB10), 5% ethylene glycol (E) + BME 2.5% (TEB2.5), TE + BME 10% (TEB10), TG and TE. Subsequently, the ejaculates were cooled to 4°C at 1.5 hours, and stored in liquid nitrogen (~-196°C). Results: The extender supplemented with 10% BME + 5% G resulted in higher progressive motility (23.19%), in comparison to the extenders supplemented with 5% EG (14.08%). Better sperm acrosome integrity was achieved with the use of the extender containing 2.5% BME + 5% G (44.99%), when compared to the use of the extender supplemented with 5% G (33.63%) (P<0.05). Conclusion: BME added to extender, may improve the postthawed sperm parameters on freezing of Kangal dog semenÖğe EFFECTS OF ARGININE AND TREHALOSE ON POST-THAWED BOVINE SPERM QUALITY(AKADEMIAI KIADO ZRT, 2017) Ozturk, Caner; Gungor, Sukru; Ataman, Mehmet Bozkurt; Bucak, Mustafa Numan; Baspinar, Nuri; Ili, Pinar; Inanc, Muhammed EnesThe present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 degrees C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 +/- 8.21%), CASA motility (12.2 +/- 5.69%) and progressive motility (3.52 +/- 2.13%), compared with the controls (43 +/- 2.73%, 55.4 +/- 6.78% and 33.48 +/- 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 +/- 3.99% and 44.1 +/- 2.18%) compared with the control (13 +/- 8.15 and 31.7 +/- 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.Öğe The effects of curcumin, ellagic acid and methionine on post-thawed Merino rams sperm parameters(FEDERATION AMER SOC EXP BIOL, 2014) Omur, Ali; Coyan, Kenan; Ozturk, Caner; Gungor, Sukru; Bucak, Mustafa[Abstract not Available]Öğe Effects of ultrasonication on damaged spermatozoa and mitochondrial activity rate(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Peker Akalin, Pinar; Baspinar, Nuri; Coyan, Kenan; Bucak, Mustafa Numan; Gungor, Sukru; Ozturk, CanerThe aim of this study was to describe an optimal sonication procedure for sperm cells. Therefore, we used several parameters such as damaged spermatozoa rate (%), mitochondrial activity rate (%), levels of lipid peroxidation, and total antioxidant potential. Ejaculates were collected from rams (n = 3) and were divided into aliquots and 3-, 6-, and 10-s duration times; 1, 3, 5, and 8 repetitive application groups were established. In the groups with 3-, 6- and 10-s duration times, with the increasing number of repeated applications, damaged spermatozoa rates increased (P < 0.05) while mitochondrial activity rates decreased (P < 0.05). In relation with sonication duration time, total antioxidant potential levels increased (P < 0.05) in single-application groups compared to those in control groups and gradually decreased as the repetitions increased. The most effective results were obtained in the group with 8 repetitions and 10-s duration based on damaged spermatozoa rate and mitochondrial activity rate.Öğe Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen(ROYAN INST, 2019) Bucak, Mustafa Numan; Bodu, Mustafa; Baspinar, Nuri; Gungor, Sukru; Ili, Pinar; Acibaeva, Begimay; Topraggaleh, Tohid Rezaei; Dursun, ŞükrüObjective: The purpose of the present study was to assess the effects of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen. Materials and Methods: In this experimental study, sixty ejaculates from six mature Merino rams were used. In experiment 1, the ejaculates were diluted in base extender contained ellagic acid at 0 (control), 0.5, 1, and 2 mM. In experiment 2, ebselen at 0 (control), 10, 20, and 40 mu M were added to the extender. Sperm motility, viability, mitochondrial membrane potential, DNA integrity, lipid peroxidation (LPO), the antioxidant potential (AOP), and total glutathione (tGSH) were evaluated at 0, 24, 48, and 72 hours of preservation. Results: Supplementation of ellagic acid at 1 and 2 mM resulted in higher sperm motility and viability at 0 hours of storage. Ellagic acid at 2 mM led to higher motility and viability compared to controls after 0, 24, and 48 hours of preservation and increased AOP after 24 and 72 hours. Higher tGSH was at 1 mM ellagic acid, compared to control after 72 hours. Addition of ebselen at a concentration of 40 mu M increased motility at 24 and 48 hours and 10 mu M produced the same effect after 48 and 72 hours of storage as well as higher viability, compared to the controls after 0 hours of storage. Sperm DNA integrity was significantly improved after 24, 48, and 72 hours with the addition of ebselen at 10 mu M, and after 72 hours at 40 mu M. Addition of 40 mM ebselen also reduced the LPO levels after 24 hours of storage compared to the controls. Conclusion: The results showed that supplementation of ellagic acid and ebselen in semen extender has a potential effect on sperm and oxidative stress parameters during liquid preservation of ram semen.Öğe Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 degrees C(ELSEVIER SCIENCE BV, 2016) Peker Akalin, Pinar; Bucak, Mustafa Numan; Gungor, Sukru; Baspinar, Nuri; Coyan, Kenan; Dursun, Sukru; Ili, PinarEjaculates were collected from six Merino rams with the aid of an artificial vagina twice a week. The ejaculates containing spermatozoa with >80% forward progressive motility and concentrations higher than 2 x 10(6) spermatozoa/ml were pooled. The present study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the Tris based extender, containing 0 (control), 0.5, 1 and 2 mM lycopene, at a final concentration of approximately 400 x 10(6) sperms/ml (single step dilution), In experiment 2, cysteamine at concentrations of 0 (control), 0.5,1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 degrees to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm and oxidative stress parameters were evaluated after 0, 24, 48 and 72 h of storage at 5 degrees C. The extender supplemented with 0.5 mM lycopene resulted in higher mitochondrial activity rate (p<0.05) in comparison to the control group at 72 h of storage. Lycopene at 0.5 mM dose led to higher sperm motility rate (p<0.05) when compared to 2 mM lycopene group at 72 h of liquid storage. As regards oxidative stress parameters, only 2 mM lycopene increased total glutathione levels (p<0.05) at 0 h of storage. The extender supplemented with 1 mM cysteamine gave higher motility (p<0.05) at 48 h compared to control. As regards oxidative stress parameters, 1 and 2 mM cysteamine at 48 h and 1 mM cysteamine at 72 h increased total glutathione levels (p<0.05) compared to control groups. Cysteamine at 1 and 2 mM doses decreased lipid peroxidation (p<0.05) at 0 h of liquid storage compared to control. Our data suggest that lycopene at 0.5 and 2 mM and cysteamine at 1 and 2 mM doses can be added to Tris based extender for improving the ram sperm motility, viability, mitochondrial activity and oxidative stress parameters during the liquid storage. (C) 2016 Elsevier B.V. All rights reserved.
