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    Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR
    (UNIV ZAGREB VET FACULTY, 2016) Sayin, Zafer; Erganis, Osman; Sakmanoglu, Asli; Hadimli, Hasan H.; Pinarkara, Yasemin; Maden, Mehmet; Al Shattrawi, Huda J. G.
    In the present study, Pasteurella caballi (P. caballi) was isolated and identified in bronchoalveolar lavage fluid and lung samples from thoroughbred Arabian foals using conventional microbiological methods. Subsequently, the ability of two different PCR primer sets was evaluated for detection and confirmation of P. caballi. Primer sets 1 and 2, targeting the 16S rRNA gene of P. caballi, were designed using the Primer 3 and Primer-BLAST programs, respectively. PCR was performed to confirm P. caballi strains and to detect it directly in the bronchoalveolar lavage fluid and lung samples. In total, 35 Pasteurella spp. were isolated from 25 (38.4 %) of 65 bronchoalveolar lavage fluid samples, and 10 (58.8 %) of 17 lung samples. These strains were identified as P. caballi based on conventional microbiological and biochemical characteristics. The sensitivities of primers 1 and 2 were determined lobe 100 % to confirm cultured P. caballi strains. However, the specificity of P. caballi detection was lower with primer set-1 than primer set-2 in bronchoalveolar lavage fluid and lung samples. The sensitivity and specificity of primer set-2 were confirmed by gene sequence analysis. This study indicates that the 16S rRNA-PCR method, using primer set-2, provides a rapid and accurate tool for the detection and confirmation of P. caballi isolates in bronchoalveolar lavage fluid and lung samples from foals.
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    Köpeklerde Solunum Yolu Hastalıklarının Kli?ni?k, Si?toloji?k, Bakteri?yoloji?k ve Radyografi?k Anali?zi?
    (Selçuk Üniversitesi, 2000) Maden, Mehmet; Birdane, Fatih M.; Alkan, Fahrettin; Şen, İsmail; Aslan, Veysi; Hadimli, Hasan H.
    Clinical, cytological, bacteriological and radiographic analysis of respiratory tract diseases were per formed in dogs. The importance of bronchoalveolar lavage (BAL) fluid and culture were analysed and it is considered to be important to detect the ethiologic factor and overall rate of inflammation. In addition to this, radiography were also evaluated if there was any contribution to the diagnosis. Thirty one dogs were used in this study of which 6 was assigned as a control group. Cough, fever, nasal discharge and abnormal auscultation were found in tracheabronchitis and bronchopneumonia. While there was a statistically significant increase (p < 0.001) in the neutrophil counts, there was a statistically significant decrease (p<0.001) on macrophage and lenfocyte counts in both groups Statistically significant increase (p < 0.05) on epithel cells counts was found Coc and/or bacils were seen in all cases Three dogs had fungal organisms, and one had anaerob bacteria Bordetella bronchiseptica and E coll (% 24) were the most isolated bacteria species in BAL fluid culture. Tracheabronchitis and bronchopneumonia findings were de termined radiographically. As a result, clinical, cytologic, bacteriologic and radiographic findings were considered to be important in the diagnosis of respiratory diseases. Especially BAL fluid cytology was found to be highly important tool in the diagnosis