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Öğe Changes in reactive oxygen species in roots of pumpkin (Cucurbita pepo L.) genotypes with known drought tolerance levels(WILEY, 2018) Hakki, E. E.; Hamurcu, M.; Ustun, C.; Avsaroglu, Z. Z.; Onay, H.; Gezgin, S.; Khan, M. K.[Abstract not Available]Öğe Determination of suitable maize (Zea mays/ L.) genotypes to be cultivated in boron-rich central anatolian soil(SPRINGER, 2007) Hakki, E. E.; Atalay, E.; Harmankaya, M.; Babaoglu, M.; Hamurcu, M.; Gezgin, S.[Abstract not Available]Öğe Evaluation of confidence interval estimates of cluster analysis in molecular marker data(ELSEVIER SCIENCE BV, 2009) Kayis, S. A.; Pinarkara, E.; Uygan, S.; Hakki, E. E.[Abstract not Available]Öğe Physiological screening of wheat genotypes grown under boron stress(ELSEVIER SCIENCE BV, 2018) Hakki, E. E.; Pandey, A.; Khan, M. K.; Hamurcu, M.; Gezgin, S.[Abstract not Available]Öğe Puccinellia distans - A potential plant to reveal boron toxicity and salt tolerance mechanisms(ELSEVIER, 2019) Hakki, E. E.; Pandey, A.; Khan, M. K.; Hamurcu, M.; Celik, O.; Gezgin, S.; Atmaca, E.; Inanc, M.; Gumus, T.; Cakir, O.; Tarhan, C.; Sameeullah, M.[Abstract not Available]Öğe Regulation of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases by basic fibroblast growth factor and dexamethasone in periodontal ligament cells(WILEY, 2009) Hakki, S. S.; Hakki, E. E.; Nohutcu, R. M.Background and Objectives: In this study, we investigated the effect of basic fibroblast growth factor (bFGF) and dexamethasone (Dex) on mRNA expressions of collagen (COL) type I, III and X, matrix metalloproteinases (MMP)-1, -2, -3 and -9 and tissue inhibitors of metalloproteinases (TIMP)-1 and -2, and also on mineralization and morphology of periodontal ligament (PDL) cells. Material and Methods: Periodontal ligament cells were obtained from premolar teeth extracted for orthodontic reasons. Periodontal ligament cells were cultured with Dulbecco's modified Eagle's medium containing: (1) 5% fetal bovine serum (FBS); (2) 5% FBS + ascorbic acid (AA, 50 mu g/mL); (3) 5% FBS + Dex (10(-7) M) + AA; (4) 5% FBS + bFGF (10 ng/mL) + AA; or (5) 5% FBS + Dex (10(-7) M) + bFGF + AA. Cells within each group were evaluated for gene expression profile using semi-quantitative reverse transcriptase-polymerase chain reaction for COL I, III and X, MMP-1, -2, -3 and -9 and TIMP-1 and -2 on days 14 and 21 and for biomineralization by von Kossa stain in vitro on day 21. Images of PDL cells were examined using a phase contrast microscope. Results: Basic fibroblast growth factor stimulated MMP-1, MMP-3 and MMP-9 mRNA expressions and inhibited TIMP-2 mRNA expression. Treatment of cells with Dex + bFGF led to downregulation of MMP-1, MMP-3 and MMP-9 transcripts. Whilst AA alone and Dex alone induced biomineralization of PDL cells, bFGF blocked the mineralization activity of the cells. In the Dex + bFGF group, more mineral nodules were noted when compared to AA alone and Dex alone groups. Conclusion: The addition of Dex to culture reversed bFGF-mediated inhibition of mineralization. Use of combined bFGF and Dex to regulate PDL cell function may be a good therapeutic option to obtain periodontal regeneration.
