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Öğe ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells(TAYLOR & FRANCIS LTD, 2009) Emecen, Pinar; Akman, Abdullah Cevdet; Hakki, Sema Sezgin; Hakki, Erdogan Esref; Demiralp, Burak; Toezeum, Tolga Fikret; Nohutcu, Rahime MeralObjective. Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro. Material and methods. PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor- (TGF-), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR. Results. ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF- and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15. Conclusions. Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing.Öğe Cytotoxicity Evaluation of Bioresorbable Fixation Screws on Human Gingival Fibroblasts and Mouse Osteoblasts by Real-Time Cell Analysis(W B SAUNDERS CO-ELSEVIER INC, 2015) Yolcu, Umit; Alan, Hilal; Malkoc, Siddik; Bozkurt, Serife Buket; Hakki, Sema SezginPurpose: To evaluate the effects of bioresorbable fixation screws (BFSs) on human gingival fibroblast (HGF) and mouse osteoblast (MC3T3-E1) cell viability. Materials and Methods: The KLS Martin SonicPins Rx, Synthes RapidSorb Cortex Screws, and Inion CPS Bioabsorbable Fixation System each were incubated in Dulbecco's Modified Eagle Medium for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200-mu L cell suspensions in the wells of an E-plate View 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the bioresorbable materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using 1-way analysis of variance and Tukey-Kramer tests. Results: There were significant differences in the HGF responses to the untreated control conditions and the Synthes (P < .01), Inion (P < .05), and KLS Martin (P < .05) treatments over 48 hours. The Synthes (P < .01) and Inion (P < .01) treatments produced lower HGF cell index values than the untreated control at 72 hours, whereas the KLS Martin treatment did not. When left to elute for 96 hours, there were no significant differences in values among the control and study groups for HGFs (P > .05). All tested BFSs decreased cell survival rates of M3T3C1 cells for 48 hours (P < .01), 72 hours (P < .001), and 96 hours (P < .001). Conclusion: Differences in the sensitivities of the 2 tested cell lines to the different BFSs might be the result of the different materials used to manufacture the screws. These results provide fundamental knowledge and new insights for the future design and development of new biocompatible BFSs for oral and maxillofacial surgery. (C) 2015 American Association of Oral and Maxillofacial SurgeonsÖğe Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis(KOREAN ACAD PROSTHODONTICS, 2015) Malkoc, Meral Arslan; Demir, Necla; Sengun, Abdulkadir; Bozkurt, Serife Buket; Hakki, Sema SezginPURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.Öğe Effect of post-restoration on stresses in premolars with endodontic-periodontal lesion: an FEA study(TAYLOR & FRANCIS LTD, 2017) Belli, Sema; Eraslan, Oguz; Hakki, Sema Sezgin; Eskitascioglu, Murat; Eskitascioglu, GuercanAim: This finite elemental stress analysis (FEA) study was aimed to test the effect of fiber and metal posts on stress distribution in premolar teeth with endodontic-periodontal (EP) lesion. Methodology: Three FEA models representing different EP lesions (primary endodontic disease (PED), PED with periodontal involvement and true-combined) were created. Tooth-model without EP lesion was used as control. The root-canals were assumed as root-filled, restored using glass-fiber or metal posts, resin composite build-up, and ceramic crown. A 300 N load was applied from the palatal surface of the crown with a 135 degrees angle. The SolidWorks/Cosmosworks structural analysis program was used for FEA analysis. Results were presented by considering von Mises criteria. Results: Maximum stress values for PED, PED with periodontal-involvement and true-combined lesions were 2.26, 1.25, 0.74 MPa for glass-fiber post; 2.08, 1.51, 1.18 MPa for metal post, respectively. If there is PED, fiber-post gave an advantage to the tooth at core structure and coronal third of the root. Metal post kept the stress inside its body however caused small, high stress concentrated areas at cervical. When there is PED with periodontal-involvement, metal post forwarded less stress toward the apical while fiber-post saved the coronal structure. In true-combined lesion model, metal post kept the stresses within its body and forwarded less stress toward the remaining root structure and periodontium. Conclusion: EP lesions have an effect on stress distribution therefore the post material selection should be done based on the severity of the lesion. Metal posts might be preferrable since it forwards less stress toward the surrounding periodontal tissues during wound healing.Öğe MMP-1, MMP-9, and TIMP-1 levels in oral lichen planus patients with gingivitis or periodontitis(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Ertugrul, Abdullah Seckin; Dursun, Recep; Dundar, Niyazi; Avunduk, Mustafa Cihat; Hakki, Sema SezginObjective: Oral lichen planus (OLP) is a chronic inflammatory disease of unknown aetiology. The aim of this study was to investigate matrix metalloproteinase (MMP)-1, MMP-9, and MMP inhibitor-1 (TIMP-1) levels in gingival crevicular fluid (GCF) by enzyme-linked immunosorbent assay and to investigate MMP-1, MMP-9, and TIMP-1 levels in gingival tissue by immunohistochemical staining of samples from patients with and without OLP. Design: Twenty-seven patients with OLP (gingivitis, OLPG; chronic periodontitis, OLPP) and thirty healthy non-OLP patients (gingivitis, HG; chronic periodontitis, HP) were included in this study. The MMP-1, MMP-9, and TIMP-1 levels in GCF were determined by enzyme-linked immunosorbent assay. The MMP-1, MMP-9, and TIMP-1 levels in gingival tissue were determined by immunohistochemical staining. Results: The mean levels of MMP-1 and MMP-9 in the GCF of OLPP patients were significantly higher and TIMP-1 was significantly lower than in HP patients; similarly, the mean levels of MMP-1 and MMP-9 were higher and TIMP-1 was significantly lower in OLPG patients than in HG patients. Our findings illustrate that tissue MMP-9 levels were statistically higher and TIMP-1 level were significantly lower in the OLPP group in comparison to the HP group, and the OLPG group in comparison to the HG group. The tissue MMP-1 level in the non-OLP group was found to be lower when compared with the OLP groups. But not statistically significant. Conclusions: Increased levels of MMP-1 and MMP-9 with decreased levels of TIMP-1 in GCF and increased MMP-1, MMP-9 levels and decreased TIMP-1 levels in the gingival tissue of OLP patients in combination with poor oral hygiene may cause increased tissue breakdown. The results of our study provide information about the effects of the periodontal status on the enzyme profiles in GCF and gingival tissue of OLP and non-OLP patients. (C) 2013 Elsevier Ltd. All rights reserved.Öğe Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis(NATURE PUBLISHING GROUP, 2013) Ertugrul, Abdullah Seckin; Arslan, Ugur; Dursun, Recep; Hakki, Sema SezginOral lichen planus (OLP) is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 (18 chronic periodontitis (OLPP) and 9 gingivitis (OLPG)) patients diagnosed with OLP along with 26 (13 chronic periodontitis (HP) and 13 gingivitis (HG)) healthy non-OLP patients. The multiplex polymerase chain reaction (PCR) with subsequent reverse hybridization method (micro-I Dent) was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients (OLPG and OLPP) were 18.5%, 85.1%, 81.4%, 88.8% and 74%, respectively. Meanwhile, in the non-OLP patients (HG and HP), these values were 7.6%, 50%, 46.1%, 73% and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens' percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathogenic microorganisms in the progress of periodontal diseases of OLP.Öğe SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)(ELSEVIER SCIENCE BV, 2017) Hakki, Sema Sezgin; Bozkurt, Serife Buket; Hakki, Erdogan Esref; Karaoz, Erdal; Unlu, Ali; Kayis, Seyit Ali[Abstract not Available]
