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Öğe Genetic Diversity of Turkish Honey Bee Populations Based on RFLPs at a Nuclear DNA Locus(CALIFORNIA STATE UNIV, 2011) Ozdil, Fulya; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. GlennGenetic diversity of Turkish honey bee populations was examined by PCR-RFLP of an anonymous nuclear DNA locus named 1231 region. The two halves of this locus, E(1)N and NE(2), were amplified from 70 bee colonies from 9 different localities. The restriction site differences responsible for the allelic RFLP patterns were determined. In one half of the 1231 region, E(1)N, was digested with BclI, HhaI and XhoI. The other half of this 1231 region, NE(2), was digested with AluI, NsiI, PstI, XhoI, HhaI and HindIII. One to seven different restriction patterns for each restriction enzyme were found in Turkish honey bees and reference samples, European and African honey bees.Öğe Mitochondrial DNA Variation in the CoxI-CoxII Intergenic Region among Turkish and Iranian Honey Bees (Apis mellifera L.)(SPRINGER/PLENUM PUBLISHERS, 2009) Ozdil, Fulya; Fakhri, Bahman; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. Glenn[Abstract not Available]Öğe Molecular characterization of Turkish honey bee populations (Apis mellifera) inferred from mitochondrial DNA RFLP and sequence results(EDP SCIENCES S A, 2009) Ozdil, Fulya; Yildiz, Mehmet Ali; Hall, H. GlennTo identify the evolutionary lineage of honey bee colonies in Turkey, the mtDNA of 244 colonies from 20 locations was analyzed. Several polymorphic restriction sites showed that they belonged to the Mediterranean C lineage. DraI digestion of the CoxI-CoxII intergenic region produced four fragment patterns, one first seen in this study. From 37 colonies from 16 different locations in Turkey and two colonies from Iran, the intergenic region was sequenced. Previously, from among all honey bee populations of the C lineage, eight haplotypes had been described from this mtDNA region, three of which were found here. In addition, eight new haplotypes were found. A deletion in one of these haplotypes accounts for the novel DraI RFLP pattern. Most of the novel haplotypes were in a subgroup of lineage C, yet none of these had been found in previous studies of Turkish honey bees. The geographical distribution of some haplotypes suggests that they may be characteristic of subspecies native to Turkey.