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    BoLA-DRB3.2 polymorphism in Holstein friesian, Simmental and Brown swiss cattle breeds
    (PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2019) Ilhan, Fatma.; Aytekin, Ibrahim.
    In this research, the BoLA-DRB3 exon 2 region in the MHC gene region was surveyed by PCR-RFLP analysis in three of the cattle breeds that are commonly reared in Turkey. This locus was PCR-amplified, and a 284-bp fragment was obtained. To determine the variation in the BoLA-DRB3.2 gene region, BstYI and HaeIII restriction enzymes were used. Three genotypes and two alleles were detected as a result of BstYl enzyme restriction; six genotypes and three alleles were detected at the end of the HaeIII enzyme fragment. The bb genotype is more frequent in all breeds as a result of BstYI restriction. Chi-square analysis revealed that the population with this enzyme was in the Hardy-Weinberg equilibrium. For the HaeIII enzyme, the bb genotype had the highest frequency, and the population is not balanced according to the enzyme cutting results. The HaeIII restriction site at 167 bp correlated with the Pro 56 amino acid. This amino acid was included in the antigen-recognition site of the MHC molecule, which was recorded in 38% of Holstein Friesian, 22% of Simmental and 19% of Brown Swiss animals. This study of BoLA-DRB3 genes in cattle breeds grown in Turkey demonstrates the genetic profile.