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    In Vitro Activity of Toxin Binders on Aflatoxin B1 in Poultry Gastrointestinal Medium
    (UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2018) Yalcin, Nihayet Fadime; Avci, Tulay; Isik, Mehmet Kursat; Oguz, Halis
    The purpose of this study was to determine the effectiveness of organic, inorganic and mixed toxin binders (TB) commonly used in poultry feeds to bind aflatoxin B1 (AFB1) in in vitro conditions. A total of 20 TBs, which were commonly used in the poultry industry, were divided into three groups as organic, inorganic and mix according to their contents. For each TB was used in in vitro poultry mediums both at pH 3 and 6.8 to represent proventriculus and intestine of poultry, respectively. Control groups were prepared from standard AFB1 and its dilutions; and the treatment groups were prepared from TBs (10mg) and standard AFB1 and its dilutions. After mixing and incubation periods, the samples (8 control, 6 organic, 6 inorganic and 8 mix) were analyzed in 4 replicates (2 for pH 3 and 2 for 6.8) by the method of Vicam aflatest method in HPLC-FLD after extraction and filtration processes. The binding capacity of inorganic TBs was found to be the highest, 98 and 93% at pH 3.0 and 6.8 while the organic TBs were the lowest, 40 and 45% at pH 3.0 and 6.8 respectively. Likewise, the binding capacity of mix TBs to AFB1 was found 96 and 88% at pH 3.0 and 6.8 in in vitro conditions. It was concluded that the inorganic and mix TBs were found to be effective to bind AFB1 among the TBs and could be applied to reduce the negative effects of AFB1 in poultry feeds. The organic TBs were found to be not sufficient to bind AFB1. (C) 2017 PVJ. All rights reserved
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    Investigation of mycotoxin residues in poultry feeds by LC MS/MS method
    (ANKARA UNIV PRESS, 2017) Yalcin, Nihayet Fadime; Isik, Mehmet Kursat; Avci, Tulay; Oguz, Halis; Yurduseven, Tuncay
    In this study, it was aimed to determine the levels of mycotoxin contamination in poultry feeds in Konya, Afyonkarahisar, Aksaray and Nigde which constitutes 26.2% of the poultry production in Turkey. For this purpose a total of 73 readyto- consume poultry feed samples from poultry farms in four provinces were collected. A general screening in feeds was performed in terms of mycotoxin presence and contamination levels. Samples were analyzed for aflatoxin B-1, ochratoxin A, zearalenone, T-2 toxin, HT-2 toxin, fumonisin and deoxynivalenol levels by LC-MS/MS multi-mycotoxin method. The levels of different mycotoxins in feeds obtained from poultry farms, the proportion of positive samples and the percentage of presence in the feeds were compared in terms of maximum residue limit (MRL). It was concluded that the feeds were found to be contaminated with low rates of mycotoxins, in terms of mycotoxin types and levels in the regions screened. However, the exceeded rates were found only 1% for aflatoxin B1 (>= 5 ppb) in poultry feeds according to communique on undesirable substances in animal feed (RG 2014/11) while other mycotoxins were below the MRL.