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    Ampelographic and Molecular Diversity among Grapevine (Vitis spp.) Cultivars
    (CZECH ACADEMY AGRICULTURAL SCIENCES, 2009) Sabir, Ali; Tangolar, Semih; Buyukalaca, Saadet; Kafkas, Salih
    This study Presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the Clustering position and diver-gence of varietal groups. The least distance was observed between Yuvarlak Cokirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.
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    Analysis of Genetic Relatedness Among Grapevine Rootstocks by AFLP (Amplified Fragment Length Polymorphism) Markers
    (WFL PUBL, 2010) Sabır, Ali; Doğan, Yıldız; Tangolar, Semih; Kafkas, Salih
    The use of appropriate grapevine rootstocks is a unique solution for preventing soil-borne problems. As commonly used rootstocks were mostly derived from different North American Puis species, each genotype has specific behavior concerning resistance to biotic and abiotic stress factors. Therefore, reliable characterization of rootstocks provides valuable knowledge to breeders in selecting proper material. In the current study, nineteen grape rootstocks were genotyped with four AFLP (Amplified Fragment Length Polymorphism) primer combinations. A total of 416 bands, of which 357 were polymorphic (86%), were generated while total number of bands per primer combination ranged from 63 to 124 with an average of 104. The pair-wise distance coefficient values were between 0.04 ('Cosmo 10' and 'Cosmo 20') and 0.41 ('Saint George' and 'Cosmo 20'). Certain genotype-specific markers were detected for '41 B' and 'Saint George' rootstocks. A dendrogram constructed from AFLP analysis formed five taxonomic groups relevant to genetic distances of genotypes belonging to certain species. On the other hand, close genetic relationship was found among the Cosmo rootstock group, with 'Cosmo 2' being the most genetically distinct. AFLP primer combinations employed in this study revealed a high degree of polymorphism and therefore, they have proven to be a powerful tool for further analysis of closely related grape genotypes.
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    Genetic Characterization of Hazelnut (Corylus avellana L.) Cultivars from Turkey Using Molecular Markers
    (AMER SOC HORTICULTURAL SCIENCE, 2009) Kafkas, Salih; Dogan, Yildiz; Sabir, Ali; Turan, Ali; Seker, Hasbi
    Genetic relationships among 18 Turkish hazelnut (Corylus avellana L.) cultivars were investigated using randomly amplified polymorphic DNA (RAPD), intersimple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) markers. Twenty-five RAPD primers, 25 ISSR primers, and eight AFLP primer pairs generated a total of 434 polymorphic marker loci. The three marker systems were able to differentiate the cultivars. Genetic similarity, index values ranged from a high of 0.96 for 'Kan' and 'UzunMusa' to a low of 0.73 for 'Yassi Badem' and 'Kalinkara'. The genetic relationships were presented as an unweighted pair group method with arithmetic average (UPGMA) dendrogram and a three-dimensional principal coordinate analysis (PCoA) plot. The UPGMA dendrogram showed two main clusters, while PCoA analysis showed three groups. Cultivar-specific markers were produced by all marker systems for 10 cultivars. This study demonstrates the usefulness of molecular markers for identification of hazelnut cultivars.
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    Molecular Characterization of Mulberry (Morus Spp.) Genotypes via RAPD and ISSR
    (Wiley-Blackwell, 2012) İpek, Muzaffer; Pırlak, Lütfi; Kafkas, Salih
    BACKGROUND: In recent years, DNA-based markers have been used quite extensively because of their many advantages over the traditional morphological and biochemical markers. Many studies have shown that molecular markers are useful in delineating the genetic relationships among closely related mulberry genotypes and cultivars. Thus, in the present study, polymer chain reaction based DNA fingerprinting techniques were used to investigate the genetic relationships among mulberry genotypes growing in different agro-climatic regions of Turkey. RESULTS: 20 RAPD primers generated a total of 173 bands, of which 157 (90.75%) were polymorphic. As for 11 ISSR primers, 124 bands (96.55%) were polymorphic in a total of 128. The similarity index for RAPD technique ranged between 0.24-0.98; 25. Is203 with 25. Is112 were found to be the closest genotypes, while 24Ke10 and 25. Is123 were the most distant ones. According to the ISSR result, the genetic similarity index changed between 0.21-095; 25. Is203 with 25. Is112 genotypes were the closest, while 25. Is08 and 01KaD2 were the most distant ones. CONCLUSION: The RAPD and ISSR markers were found to be promising for assessing genetic diversity in mulberry genotypes. (C) 2011 Society of Chemical Industry