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    The Effect of Melatonin on Sperm Quality and Testicular Size of Normospermic and Pathospermic Rams in the Anoestrous Season
    (OSTAG-WERBUNG & VERLAG, 1998) Çoyan, K; Kaya, A; Karaca, F; Ataman, M B; Yıldız, C
    The aim of this study was to investigate the effects of melatonin implants on sperm quality and testicular size of normospermic and pathospermic rams in the anoestrous season. The study was carried out on a total of fourteen Konya Merino rams. As a consequence of preliminary experiments, eight rams were found as normospermic and six rams as pathospermic. Both the normospermic and the pathospermic rams were divided into two groups with regard to spermatologic features and testicular size. Four normospermic and three pathospermic rams were treated with melatonin implant (Regulin(R)) and control groups were sham-treated. Semen samples of each ram were started to be collected after 5 weeks of the melatonin applications twice a week for 6 weeks. Semen samples were examined for volume, individual motility, sperm concentration, live/dead and abnormal sperm rates. Scrotal circumference and testes volume of the rams were measured within 15-day intervals during the study. Sperm motility and abnormal spermatozoon rates were found to be higher in the semen samples obtained from normospermic melatonin implanted rams than those of normospermic control rams. Significant variations in all spermatologic characteristics studied were found between pathospermic melatonin implanted rams and those of pathospermic control rams. Scrotal circumferences and testes volumes were found to be significantly higher in all melatonin implanted rams than in control rams. Our results indicated that melatonin implants have positive effects on sperm quality and increased the scrotal circumference and testes volume of both normospermic and pathospermic rams in anoestrous seasons.
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    Estimation of early pregnancy by electrical resistance values of vaginal mucosa in cows and heifers
    (ECOLE NATIONALE VETERINAIRE TOULOUSE, 2005) Taşal, İbrahim; Ataman, Mehmet Bozkurt; Aksoy, Melih; Kaya, Abdullah; Karaca, F; Tekeli, Tevfik
    The aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF(2 alpha), was administered intramuscularly twice (500 mu g) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 11 19 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also collected on days 19, 20 and 21 for cows, and 18, 19 and 20 for heifers. Pregnant animals were detected by rectal palpation 60 days after insemination. In pregnant cows, electrical resistance of vaginal mucosa measured between days 19-21 was significantly higher than non-pregnant animals, while vaginal resistance values determined on the day of insemination, and on days 3, 6, 10 and 13 post-insemination were similar in both groups. Likewise, vaginal impedance readings in pregnant heifers determined between days 18-20 were significantly higher than those non-pregnant animals. Vaginal resistance measurements in cows (between days 19-21) and heifers (between days 18-20) were significantly correlated with plasma progesterone concentrations both in pregnant and non-pregnant groups. It was concluded that vaginal resistance values might be used for the diagnosis of early pregnancy as a contributor or predictor method in cows and heifers.