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Öğe Biohydrogen and 5-aminolevulinic acid production from waste barley by Rhodobacter sphaeroides O.U.001 in a biorefinery concept(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Kars, Gokhan; Ceylan, AycaThe production of biohydrogen and 5-aminolevulinic acid (5-ALA) by Rhodobacter sphaeroides O.U.001 was investigated in a biorefinery concept. Waste barley was used as a substrate after acid hydrolysis. The hydrolysate was analyzed in terms of its total simple sugar, organic acid, ammonium, element and total phenol contents. Four different growth media having 5 g/L, 7 g/L, 9 g/L and 11 g/L sugar content were prepared using the waste barley hydrolysate to produce biohydrogen and 5-ALA. The increased sugar concentrations resulted in higher cell density and hydrogen accumulation. Accordingly, the highest cell density (OD660: 1.78) and hydrogen production (0.4 L H-2/L culture) were observed in the 11 g/L sugar-containing medium. A 67.4 mu M 5-ALA was produced upon vitamin B-12 and levulinic acid additions. These results showed that waste barley can be used as a substrate for R. sphaeroides for biohydrogen and 5-ALA production within a biorefinery concept. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.Öğe Effect of Nerium oleander distillate on MCF-7 breast cancer cell lines(CURRENT BIOLOGY LTD, 2011) Kars, Meltem Demirel; Kars, Gokhan; Gunduz, Ufuk; Uney, Kamil; Bas, Ahmet Levent[Abstract not Available]Öğe Implications from a pharmacogenomic analysis: Nerium oleander leaf distillate supplemented diet regulates cholesterol metabolism in rats(TAYLOR & FRANCIS LTD, 2014) Kars, Meltem Demirel; Odabasi, Burcu Asena; Kars, Gokhan; Uney, Kamil; Bagci, Yavuz; Bas, Ahmet LeventContext: Despite the usage of Nerium oleander L. (Apocynaceae) for anticancer studies and traditional remediation, the regulatory effect of N. oleander leaf distillate on cholesterol metabolism is not disclosed sufficiently. Objective: Cholesterol is an important biological molecule and the synthesis rate is regulated by the amount of cholesterol uptake from the diet. The aim of this study was to investigate the regulation of cholesterol metabolism in response to a high-fat diet (HFD) and the effects of N. oleander leaf distillate-supplemented diet (NOHFD) in rats. Materials and methods: Microarray technology was used to clarify the regulation of cholesterol mechanism in HFD and NOHFD-fed rats (375 mu g/0.5 mL distilled water applied by gavage). The treatment period was 90 days. Rat liver tissues were used for microarray analysis using the Affymetrix GeneChip Rat Genome platform. Results of groups were statistically analyzed with the Partek 6.6 bioinformatic program. Results: The HFD group exhibited alterations in the expression levels of about 1945 genes with respect to the normal diet (ND) group. The results showed that expression levels of 47 genes were altered related to cholesterol metabolism in HFD and NOHFD groups. The expression levels of seven genes in the NOHFD group were significantly closer to those in the ND group than those of the HFD group. Discussion and conclusion: To conclude, findings suggest that N. oleander leaf distillate-supplemented food has considerable beneficial effects on cholesterol metabolism-related gene expression levels.Öğe Paclitaxel Resistance in MCF-7/Pac Cell Line is Reversed Successfully by Saikosaponin A and Saikosaponin D(AKAD DOKTORLAR YAYINEVI, 2013) Kars, Meltem D.; Kars, Gokhan; Gunduz, UfukCancer cells demonstrate multiple drug resistance phenotype frequently after chemotherapy. The resistance of cancer cells to various chemotherapeutic agents is defined as multiple drug resistance. The purpose of this study is to investigate the potential reversal effects of active agents, that are found high amount in plants, on resistant MCF-7 cell lines. The effects of potential MDR modulators combined with anticancer drugs were also evaluated. Flow cytometry, fluorescence microscopy and checkerboard combination assays were performed to study the reversal of drug resistance and for investigation of the antiproliferative effects of the combination of anticancer drugs with the modulators. Paclitaxel and potential MDR modulators (verapamil, saikosaponin A, D and isoquercitrin) were applied to the sublines in combination. Fluorescence accumulation levels and fractional inhibitory indices show that saikosaponin A and D are effective MDR reversal agents that may be used together with paclitaxel in drug resistant mammary carcinoma subline. In conclusion this report represents saikosaponin A and D from natural resources are valuable reagents that may improve the success of chemotherapy.Öğe Photofermentative biohydrogen production from barley waste(ELSEVIER SCIENCE BV, 2012) Kars, Gokhan; Ceylan, Ayca[Abstract not Available]Öğe Saikosaponin A and saikosaponin D exert significant drug resistant reversal activity on paclitaxel resistance(ELSEVIER SCIENCE BV, 2012) Kars, Gokhan[Abstract not Available]Öğe Unraveling the 5-aminolevulinic acid biosynthesis genes in Rhodobacter sphaeroides OU001(ELSEVIER SCIENCE BV, 2016) Kars, Gokhan; Ridha, Farmesk; Jawad, Marwa[Abstract not Available]
