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  • Küçük Resim Yok
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    Erythrocyte and spermatozoa glucose-6-nhosphate dehydrogenase activity in merino rams: An experimental study
    (SHAHID SADOUGHI UNIV MEDICAL SCIENCES, 2018) Gürel, Hüseyin; Başpınar, Nuri; Akalın, Pınar Peker; Altunok, Vahdettin; Kazak, Filiz
    Background: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (x10(6)), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p <= 0.001), whereas spermatozoa G6PD activity was lower (p<0.001) in the breeding season (1.928 +/- 0.231 U/g hemoglobin, 129.65 +/- 28.41 U/g protein, respectively) from that in the non-breeding (0.530 +/- 0.066 U/g hemoglobin, 562.36 +/- 94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance.
  • Küçük Resim Yok
    Öğe
    Erythrocyte and spermatozoa glucose-6-phosphate dehydrogenase activity in merino rams: An experimental study
    (Research and Clinical Center for Infertitlity, 2018) Gürel, Hüseyin; Başpınar, Nuri; Akalın, Pınar Peker; Altunok, Vahdettin; Kazak, Filiz
    Background: Glucose-6-phosphate dehydrogenase (G6PD) is the first enzyme of the pentose phosphate metabolic pathway that supplies reducing agents by maintaining the level of reduced nicotinamide adenine dinucleotide phosphate. Objective: It was aimed to determine the activity of erythrocyte and spermatozoa G6PD in the breeding and non-breeding seasons in Merino rams. And also, to find out the relation of these parameters with sperm quality parameters for better understanding the role of this enzyme in male fertility. Materials and Methods: 1.5-2 yr-old healthy, 14 Merino rams were involved. Ejaculate samples were collected using an artificial vagina, in October (the breeding season) and April (the non-breeding season). Blood samples were collected prior to sperm collection. Sperm volume (ml), motility (%), mass activity (1-5), concentration (×106), viability (%), abnormal acrosome morphology (%) and abnormal sperm morphology (%) was evaluated. The activities of spermatozoa and erythrocyte G6PD were determined and the relation of sperm parameters with G6PD activity was evaluated. Results: Erythrocyte G6PD activity was higher (p?0.001), whereas spermatozoa G6PD activity was lower (p?0.001) in the breeding season (1.928±0.231 U/g hemoglobin, 129.65±28.41 U/g protein, respectively) from that in the non-breeding (0.530±0.066 U/g hemoglobin, 562.36±94.92 U/g protein, respectively). There were also significant differences among sperm quality parameters within the seasons. Positive correlation was determined between spermatozoa G6PD activity (r=0.053, p=0.03 and sperm concentration in the breeding season. Conclusion: Higher spermatozoa G6PD activity in October, where the level of polyunsaturated fatty acids is suggested to be increased, may reflect the increased need of nicotinamide adenine dinucleotide phosphate and thus higher G6PD activity for the oxidative balance. © 2018, Research and Clinical Center for Infertitlity. All rights reserved.
  • Küçük Resim
    Öğe
    Malondialdehyde and Antioxidant Content of Pasteurized, Semi-Skimmed UHT and UHT Milks
    (Selçuk Üniversitesi, 2023 Mart) Kazak, Filiz
    Amaç: Bu çalışmada pastörize, yarım yağlı UHT ve UHT sütlerin oksidan ve antioksidan parametrelerinin değerlendirilmesi için malondialdehit (MDA), vitamin (Vit) C, total protein (TP), redükte glutatyon (rGSH) ve katalaz (CAT) belirlenmesi ve bu parametreler arasındaki korelasyonların ortaya çıkarılması amaçlandı. Gereç ve Yöntem: Hatay ilinde farklı marketlerden farklı markalara ait 30 adet süt örneği toplandı. Süt MDA, Vit C, TP, rGSH düzeyleri ve CAT aktiviteleri spektrofotometrik yöntemlerle belirlendi. Bulgular: En düşük MDA düzeyi yarım yağlı UHT sütte belirlendi (p < 0,05). Pastörize sütün MDA düzeyi yarım yağlı UHT sütten daha yüksekti (p < 0,05). Pastörize sütlerde Vit C (p < 0,001) ve TP (p < 0,01) düzeyleri diğer sütlere göre daha yüksek bulundu. Pastörize ve UHT sütlerin rGSH düzeyleri, yarım yağlı UHT sütlere göre daha yüksekti (p < 0,05). UHT yarım yağlı süt CAT aktivitesi diğer sütlere göre oldukça yüksek bulundu (p < 0,05). Korelasyonlar açısından; pastörize süt MDA seviyesi, Vit C seviyesi ile pozitif korelasyon gösterdi (r = 0,743, p < 0,01). Yarım yağlı UHT süt TP seviyesi, CAT aktivitesi ile pozitif korelasyon gösterdi (r=0,673, p < 0,05). Öneri: Sonuç olarak sunulan çalışmada, pastörize, yarım yağlı UHT ve UHT sütün oksidan (MDA), antioksidan parametreleri (Vit C, rGSH, and CAT), ve TP düzeyleri değerlendirildi ve tartışıldı. Bu çalışmada araştırılan değerler açısından, pastörize süt tüketiminin diğer ticari sütlere göre daha sağlıklı olabileceği önerilmektedir.