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    Evaluation of h beta D-1 and h beta D-2 Levels in Saliva of Patients with Oral Mucosal Diseases
    (UNIV WEST INDIES FACULTY MEDICAL SCIENCES, 2013) Kucukkolbasi, H.; Kucukkolbasi, S.; Ayyildiz, H. F.; Dursun, R.; Kara, H.
    Objective: This study aimed to determine a possible correlation between oral mucosal disease and salivary concentrations of the antimicrobial peptides human beta-defensin-1 (h beta D-1) and human beta-defensin-2 (h beta D-2). Method: The present work focussed on the establishment of a reversed phase-high performance liquid chromatography (RP-HPLC) procedure to quantify human beta-defensins (h beta D-1 and h beta D-2) in saliva samples of patients with oral diseases such as lichen planus (n = 10), Behcet (n = 10) and recurrent apthous stomatitis (n = 10). Results: Linear calibration range for h beta D-1 and h beta D-2 defensins was 1.67-200 mu g mL(-1) and 3.13 - 100 mu g mL(-1) with R-2 values of 0.9998 and 0.996, correspondingly. The concentration of beta-defensins in saliva was determined by comparing the peak areas of eluted h beta D-1 and h beta D-2 with that of their standards. The variation of the amount of beta-defensins was evaluated by comparisons of the results obtained from the patients with oral mucosal diseases before and after treatments and the control subjects. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.62 mu g mL(-1) and 5.39 mu g mL(-1) for h beta D-1 and 0.94 mu g mL(-1) and 3.13 mu g mL(-1) for h beta D-2, respectively. Conclusion: The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients with oral mucosal diseases, the concentration was significantly higher before treatment than after treatment.
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    A Highly Sensitive Ascorbic Acid Sensor Based on Graphene Oxide/CdTe Quantum Dots-Modified Glassy Carbon Electrode
    (PLEIADES PUBLISHING INC, 2019) Kucukkolbasi, S.; Erdogan, Z. O.; Baslak, C.; Sogut, D.; Kus, M.
    Quantum dots (QDs) and graphene oxide (GO) are extremely attractive and important nanomaterials in analytical applications because of their their unusual chemical, physical and electronic properties. In this work, CdTe QDs with the size of about 3 nm were prepared and a novel electrochemical sensing material of ascorbic acid on GO/CdTe QDs/GC electrode was explored. Transmission electron microscopy (TEM) was used to examine the morphological characterization of CdTe QDs and cyclic voltammetry (CV) and electrochemical impedance spectroscopy were used to perform the electrochemical investigations of the GO/CdTe QDs/GC electrode. Because of the synergy between the CdTe QDs and GO, this novel sensing based on CdTe QDs/GO/GC electrode responded even more sensitively and selectively than that based on bare GC electrode. Effects of pH value, buffer concentration, deposition potential and deposition time and electroactive interferents on the response of GO/CdTe QDs/GC electrode for ascorbic acid sensor were discussed. Under optimum working conditions, a linear response of the modified electrode was obtained over the concentration range of 32.3-500.0 mu M with the detection limit of 6.1 mu M for ascorbic acid. Finally, the inexpensive, reliable and sensitive modified electrode based on GO/CdTe QDs/GC was succesfully applied for the determination of ascorbic acid in citrus samples.