Yazar "Kurar E." seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Comparison of five different rna isolation methods from equine endometrium for gene transcription analysis(Veteriner Fakultesi Dergisi, 2010) Kurar E.; Atli M.O.; Güzeloğlu A.; Özşensoy Y.; Semacan A.In this study, five different isolation protocols to extract total RNA from biopsies of equine endometrium were compared in terms of quality and quantity of RNA samples with respect to downstream gene transcription analysis, such as Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Three phenol-chloroform based protocols (TRIzol, TRItidy, EZ-RNA) and two column based protocols (UltraClean™ and E.Z.N.A.®) that were commercially available were used. Each protocol yielded good quality total RNA and distinct 28S and 18S rRNA bands were observed in agarose gel electrophoreses. Amount of total RNA isolated was lower for EZ-RNA protocol. Column based protocols had RNA contaminated with great amount of genomic DNA, however, DNAse-I digestion was able to fully clean the DNA contamination from RNA in all the protocols used. Following cDNA synthesis and PCR, GAPDH, a housekeeping gene, bands were amplified from all the samples. In conclusion, all the protocols used extracted good quality but different amounts of total RNA and it is strongly recommended that RNA samples must undergo DNAse-I digestion before RT-PCR to eliminate gDNA contamination.Öğe Expression of epidermal growth factor (egf) and heparin-binding egf (hb-egf) mrna in mare endometrium(Veteriner Fakultesi Dergisi, 2012) Atli M.O.; Güzeloğlu A.; Kurar E.; Kayiş S.A.; Aslan S.; Semacan A.; Çelik S.EGF and HB-EGF play crucial roles in embryonic development and peri-implantation. In this study, aim was to characterize expression profiles of EGF and HB-EGF in mare endometrium during estrous cycle and early pregnancy. Endometrium biopsies were obtained from mares on day of ovulation (d0), late diestrus (LD) and after luteolysis (AL) in the estrus phase. In pregnant groups, biopsies were taken on days 14 (P14), 18 (P18), 22 (P22) and 60 (P60). Relative expression levels of genes were quantified using real-time RT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significantly different group(s). EGF mRNA expression was up-regulated at LD compared to d0 while HB-EGF expression was not changed throughout the cycle. EGF expression was also increased during early pregnancy with the highest expression level observed on P60. Similarly, HB-EGF mRNA level was increased on P60. Pregnancy induced EGF expression on P14 and P18 compared to LD and AL whereas expression of HB-EGF was only significantly higher on P18 than that of AL. These results indicate that EGF expression is up-regulated during the cycle at late diestrus when P4 is high and is increased by pregnancy. HB-EGF expression is induced later in the pregnancy. In conclusion, EGF and HB-EGF appear to involve in the events that happen in the mare endometrium during peri-implantation period.Öğe Genetic diversity of native Turkish cattle breeds: Mantel, AMOVA and bottleneck analysis(Network for the Veterinarians of Bangladesh, 2014) Özšensoy Y.; Kurar E.This study was conducted to evaluate potential extinction risk of Turkish native cattle breeds using Mantel and AMOVA tests and Bottleneck analysis. A total of 271 DNA samples were isolated from Anatolian Black, Anatolian Grey, South Anatolian Red, Native Southern Anatolian Yellow, East Anatolian Red, and Zavot cattle. In this study, genotypes of 20 microsatellites were determined by capillary electrophoresis and fragment analysis. A total of 269 different alleles were detected. The maximum and minimum numbers of total alleles were observed in TGLA122 (n=26) and INRA005 (n=7) loci, respectively. The highest average observed and expected heterozygosity values were determined as 0.619-0.852 and 0.669-0.877, respectively. The average FIS value was 0.068. Results of AMOVA and Mantel tests illustrated statistically significant differences in populations (p < 0.001) and correlation (p < 0.01). Bottleneck analysis revealed a normal distribution of L-shaped curve indicating that there was no recent risk of extinction for these breeds.Öğe Investigation of genetic diversity and paternity in kangal white karaman rams using microsatellite markers(Veteriner Fakultesi Dergisi, 2012) Kurar E.; Bulut Z.; Çağlayan T.; Garip M.; Yilmaz A.; Nizamlioğlu M.The objectives of this study were to estimate genetic diversity and test possibility of conducting paternity testing at the DNA level as part of a breeding project of Kangal White Karaman sheep. As a pilot study, blood samples were collected from 13 rams that were used for breeding purposes in two different flocks in which level of inbreeding is proposed to be high. A total of 20 microsatellite markers were used to amplify genomic DNA by polymerase chain reaction (PCR). The resulting PCR products were separated by capillary electrophoresis and allele genotypes were determined. A total of 99 different alleles were determined ranging from 1 to 8 at each locus. The observed (Ho) and expected heterozygosities (He) values were ranged from 0.000 to 0.923 and from 0.000 to 0.871, respectively. Polymorphism information content (PIC) values were between 0.000 and 0.818. Total power of exclusion (PE) value was calculated as 0.999975 for twenty loci. Our results suggested that a panel, including the most informative twelve loci provides a total PE value of 0.999828, can be useful for parentage testing in Kangal White Karaman sheep.Öğe Marker systems and applications in genetic characterization studies [Markör Sistemleri ve Genetik Karakterizasyon Çalışmalarında Kullanımları](Halic Universitesi Fen-Edebiyat Fakultesi, 2012) Özşensoy Y.; Kurar E.Nowadays, owing to the developments in molecular biology, genetic markers are generally used to describe specific regions of the genome. Three different marker systems, namely, protein and DNA markers, are used in genome analyses and in various genetic studies. Following the discovery of polymerase chain reaction (PCR), PCR-based marker systems are widely preferred in genetic studies. Genetic characterization studies are critically important to determine the level of genetic diversity between and within populations, origin of domestication and migration and development of conservation programs. Different biochemical marker systems, alloenzymes, mitochondrial DNA and Y chromosome are used for genetic characterization studies. DNA markers, especially the polymorphic microsatellite markers, are the most preferable marker systems in PCR applications. Recent progresses in molecular biology techniques allow rapid and economical identification of single nucleotide polymorphisms analyses and their applications along with microsatellites.
