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Öğe Genetic Diversity of Turkish Honey Bee Populations Based on RFLPs at a Nuclear DNA Locus(CALIFORNIA STATE UNIV, 2011) Ozdil, Fulya; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. GlennGenetic diversity of Turkish honey bee populations was examined by PCR-RFLP of an anonymous nuclear DNA locus named 1231 region. The two halves of this locus, E(1)N and NE(2), were amplified from 70 bee colonies from 9 different localities. The restriction site differences responsible for the allelic RFLP patterns were determined. In one half of the 1231 region, E(1)N, was digested with BclI, HhaI and XhoI. The other half of this 1231 region, NE(2), was digested with AluI, NsiI, PstI, XhoI, HhaI and HindIII. One to seven different restriction patterns for each restriction enzyme were found in Turkish honey bees and reference samples, European and African honey bees.Öğe Identification of factor XI deficiency in Holstein cattle in Turkey(BIOMED CENTRAL LTD, 2009) Meydan, Hasan; Yildiz, Mehmet A.; Ozdil, Fulya; Gedik, Yasemin; Ozbeyaz, CeyhanBackground: Factor XI (FXI) is a plasma protein that participates in the formation of blood clots. Factor XI deficiency is autosomal recessive hereditary disorder that may be associated with excess bleeding in Holstein cattle. Methods: In this study, 225 Holstein cows reared in Turkey were screened in order to identify FXI genotypes. DNA extractions were obtained from the fresh blood of the cows. Amplicons of FXI exon 12 were obtained by Polymerase Chain Reaction (PCR), and analyzed by 2% agarose gel electrophoresis stained with ethidium bromide. Additionally, all cows were confirmed by DNA sequencing to determine whether or not there was a mutant allele. Results: Carriers of the FXI deficiency have two DNA fragments of 320 bp and 244 bp in size. The results of our study demonstrated that only four out of the 225 Holstein cows tested in Turkey carried the FXI deficiency. The frequency of the mutant FXI allele and the prevalence of heterozygous cows were found as 0.9% and 1.8%, respectively. Conclusion: The DNA-based test determines all genotypes, regardless of phenotype or FXI activity. The mutation responsible for the FXI deficiency had not been detected in Holstein cattle in Turkey before prior to this study. The frequency of the mutant FXI allele needs to be confirmed by carrying out further analyses on cattle in Turkey and the selection programs should be developed to eliminate this genetic disorder.Öğe Mitochondrial DNA Variation in the CoxI-CoxII Intergenic Region among Turkish and Iranian Honey Bees (Apis mellifera L.)(SPRINGER/PLENUM PUBLISHERS, 2009) Ozdil, Fulya; Fakhri, Bahman; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. Glenn[Abstract not Available]
