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Interaction of MC3T3-E1 cells with titanium implants
(TURKISH JOINT DISEASES FOUNDATION, 2008) Korkusuz, Petek; Hakki, Sema S.; Purali, Nuhan; Gorur, Ilker; Onder, Ercuement; Nohutcu, Rahime; Koc, Nursen
Objectives The aim of the present study was to evaluate in-vitro MC3T3-E1 preosteoblastic cell osseointegration on surfaces of polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Materials and methods MC3T3-E1 cell proliferation and mineralization was assessed comparatively on polished, sand-blasted (smooth and rough) and sodium titanate coated titanium alloys. Cell morphology, attachment and proliferation were also comparatively evaluated using confocal (CM) and scanning electron microscopy (SEM). Results All implants Used in this study were biocompatible. Cells started to attach on the surfaces of the implants following exposure to the in vitro medium for 3 days. The cells were viable and metabolically active as observed by CM. Cell Population increased exponentially from day 3 to day 22. Proliferation rate was highest on polished surfaces and lowest on sodium titanate-coated surfaces. In contrast, mineralized nodules were numerous on sand-blasted and sodium titanate-coated Surfaces when compared to the polished ones on day 30. Conclusion This study demonstrated that sand-blasting and sodium titanate coating provided by NaOH favored the attachment, mineralization and early differentiation of osteoblasts on titanium alloys.