Yazar "Ogut M." seçeneğine göre listele

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    Phosphate solubilization potentials of acinetobacter strains and their relations with soil properties
    (2010) Ogut M.; Er F.; Kandemir N.
    Phosphate solubilizing bacteria can be used as soil or seed inoculum to increase soil phosphorus (P) availability for agricultural purposes. There is also a possibility of using these microorganisms to biotechnologically dissolve phosphate ores for the production of phosphorus fertilizers. Twenty-one soil samples were collected along a highway in Turkey to isolate phosphate solubilizing bacteria. A total of 20 phosphate solubilizers were isolated from the rhizosphere of wheat and maize grown in the pots, which contained the collected soil samples. The isolates were distributed among the genera, Acinetobacter (7), Pseudomonas (7), Enterobacter (2), Enterococcus (1), Escherichia (1), Photorhabdus (1), and Bacillus (1) as determined by the 16S rDNA gene sequence analysis. Since the Acinetobacter species were most effective in Pikovskaya's agar, which contained tricalcium phosphate for the sole P-source, they were further experimented for the phosphate solubilization in batch cultures. The mean phosphorus dissolved in 5 day incubation ranged between 167 and 1022 ppm P. The initial pH of 7.8 dropped below 4.7 in six isolates with a gluconic acid production in the concentrations ranging between 27.5 and 37.5 mM. Acinetobacter isolates have some potential as an inoculum both for soil and biotechnological Psolubilization. © 2010 al-Farabi Kazakh National University.
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    Survival of a phosphate solubilizing microorganism in ion-sterile carriers
    (2010) Er F.; Ogut M.
    A cold-tolerant phosphate solubilizing bacterium (PSB) was isolated from roots of ryegrass (Lolium perenne L.). Studies involving phosphate solubilization in liquid culture and survival of the PSB in nonsterile zeolite, leonardite, peat, rock phosphate, and an organic fertilizer were performed. The PSB was able to dissolve 163 ppm P with a simultaneous fall in pH (from 7.7 to 5.7) in Pikovskaya's medium during a ten-day incubation. The number of PSB declined logarithmically in 28 °C incubation regardless of the carrier. The rate of decrease in PSB population was less pronounced in zeolite. However, the PSB's population density increased up to 10 9 cfu g -1, and stayed in the range of 10 8 to 10 9 cfu g -1 in zeolite and rock phosphate after 13-weeks of storage at +4 °C. The contaminant microorganisms also grew in the carriers, with population densities ranging between 10 8 to 10 9 cfu g -1 at week-9. The suppression of the local microorganisms is required to increase the quality of organic fertilizer by the addition of PSB. Zeolite could be a good carrier, due to its large surface area and porosity, which allow high number of microorganisms to occupy. © 2010 al-Farabi Kazakh National University.