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Öğe Antifungal activity of several root canal sealers against Candida albicans(TAYLOR & FRANCIS LTD, 2013) Ozcan, Erhan; Yula, Erkan; Arslanoglu, Zeki; Inci, MelekObjective. The purpose of this in vitro study was to evaluate the antifungal activity of several root canal sealers (iRoot SP, MTA Fillapex and GuttaFlow) against Candida albicans and compare them to that of AH Plus Jet. Materials and methods. A10 mu L fungi in suspension was allowed to directly contact the sealers, which were freshly mixed or allowed to set for 1 or 7 days. Fresh media was then added and survival of fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. After incubation for 48 h, colony-forming units (CFU) were calculated and their log(10) values converted. The data were analyzed using ANOVA and Tukey tests (alpha = 0.05). Results. Freshly mixed AH Plus Jet totally inhibited the growth of fungi and showed the highest antifungal activity. GuttaFlow did not show any significant antifungal activity at all times. Freshly mixed iRoot SP and MTA Fillapex were found to be antifungal. Statistical differences were found between freshly mixed and set samples (p < 0.05) in favor of the former, except GuttaFlow. No statistically significant differences were found among the tested sealers at the 1 and 7-day samples (p > 0.05). Conclusions. Fresh AH Plus Jet had very potent antifungal activity. All sealers, except GuttaFlow, exhibited antifungal activity when freshly mixed.Öğe Antimicrobial effects of several calcium silicate-based root-end filling materials(JAPANESE SOC DENTAL MATERIALS DEVICES, 2014) Damlar, Ibrahim; Ozcan, Erhan; Yula, Erkan; Yalcin, Muhammet; Celik, SalihThe purpose of this study was to evaluate, in vitro, the antimicrobial effect of iRoot BP, iRoot BP Plus, and mineral trioxide aggregate (MTA) against Entercoccus faecalis and Candida albicans by using direct contact test. The materials were tested immediately after application to the microtiter wells and after setting for 1-day and for 7-days. Ten microliters of microbial suspension was added to each well for direct contact with each material for 1 h at 37 degrees C and 100% humidity. Then fresh media was added and, survival of bacteria and fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. In fresh and 1-day samples all of tested materials showed statistically significant antimicrobial effects compared to control groups (p<0.05). In 7-day samples, there were no significantly differences compared to control groups. MTA, iRoot BP and iRoot BP Plus had similar antimicrobial efficacy against E. faecalis and C. albicans.Öğe Assessment of the sealing abilities of several root canal sealers and filling methods(TAYLOR & FRANCIS LTD, 2013) Ozcan, Erhan; Eldeniz, Ayce Unverdi; Aydinbelge, Hale AriObjective. To evaluate the sealing abilities of two root canal sealers (epoxy resin-based AH Plus (R) and polydimethylsiloxane-based GuttaFlow (R)) and of five root filling techniques (lateral condensation, matched taper single gutta-percha point, laterally condensed-matched taper gutta-percha point, Thermafil (R) and continuous wave of condensation), using a bacterial leakage model. Materials and methods. One hundred and seventy-four single-rooted human teeth were randomly divided into 10 test groups (n = 15) and two control groups (n = 12). The roots that were filled with the test material, using the different root filling techniques, were mounted in a two-chamber bacterial leakage model and Enterococcus faecalis was added to the upper chambers. The lower chambers of all of the specimens were checked every day during the test period (100 days). The day of turbidity was recorded for each sample. Statistical analysis was performed using the Kaplan-Meier and log-rank tests. Results. There was no significant difference between the epoxy resin-based and the polydimethylsiloxane-based sealers, irrespective of the filling techniques used (p > 0.05). The continuous wave of condensation technique was found to be superior to the other techniques (p < 0.05). The difference between the other groups was insignificant (p > 0.05). Conclusions. AH Plus and GuttaFlow sealers showed similar levels of sealing ability. The continuous wave of condensation technique had the best sealing capability when compared to the other techniques.Öğe A comparative evaluation of sealing ability of a new, self-etching, dual-curable sealer: Hybrid Root SEAL (MetaSEAL)(MOSBY-ELSEVIER, 2008) Belli, Sema; Ozcan, Erhan; Derinbay, Oznur; Eldeniz, Ayce UnverdiObjective. To assess the long-term sealing ability of a new dual-curable, self-etching, 4-META containing resin-based sealer: Hybrid Root SEAL (MetaSEAL in the United States) and compare with RealSeal and AH Plus sealers. Methodology. Root canals of 44 extracted and decoronated single-rooted human teeth were instrumented using a crown-down technique with ProFile 0.04 tapered NiTi rotary instruments to ISO size 30 and then to size 45 with K-hand files. Four roots were selected and used as positive and negative controls (n = 2), the rest were randomly divided into 4 groups (n = 10) and filled using 0.04 tapered size 45 cones as follows: Group 1: AH Plus with gutta-percha; Group 2: Hybrid Root SEAL (MetaSEAL) with gutta-percha; Group 3: Hybrid Root SEAL (MetaSEAL) with Resilon point; and Group 4: RealSeal with Resilon point. The quality of seal of each specimen was measured after 1, 4, 12, and 24 weeks using a fluid transport model. Measurements were made at 2-minute intervals for 8 minutes. The data were calculated as Lp and statistically analyzed using 2-way repeated measures of ANOVA and Bonferroni pairwise comparison tests (alpha = 0.05). Results. There were no significant differences among test materials in terms of fluid microleakage values (P = .126). There were statistically significant differences among the time periods (P = .009) and observed between 1- and 24-week test periods. There was significant interaction between root canal filling materials and time of testing (P = .048). Conclusion. Within the limitations of this in vitro study, it was concluded that recently introduced Hybrid Root SEAL (MetaSEAL) showed similar sealing performance with RealSeal and AH Plus sealers when used either with gutta-percha or Resilon at 24 weeks. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106: e45-e52)Öğe The effect of a diode laser and traditional irrigants on the bond strength of self-adhesive cement(KOREAN ACAD PROSTHODONTICS, 2013) Tuncdemir, Ali Riza; Yildirim, Cihan; Ozcan, Erhan; Polat, SerdarPURPOSE. The purpose of this study was to compare the effect of a diode laser and traditional irrigants on the bond strength of self-adhesive cement. MATERIALS AND METHODS. Fifty-five incisors extracted due to periodontal problems were used. All teeth were instrumented using a set of rotary root canal instruments. The post spaces were enlarged for a No.14 (diameter, 1.4 mm) Snow light (Abrasive technology, OH, USA) glass fiber reinforced composite post with matching drill. The teeth were randomly divided into 5 experimental groups of 11 teeth each. The post spaces were treated with the followings: Group 1: 5 mL 0.9% physiological saline; Group 2: 5 mL 5.25% sodium hypochlorite; Group 3: 5 mL 17% ethylene diamine tetra acetic acid (EDTA), Group 4: 37% orthophosphoric acid and Group 5: Photodynamic diode laser irradiation for 1 minute after application of light-active dye solution. Snow light posts were luted with self-adhesive resin cement. Each root was sectioned perpendicular to its long axis to create 1 mm thick specimens. The push-out bond strength test method was used to measure bond strength. One tooth from each group was processed for scanning electron microscopic analysis. RESULTS. Bond strength values were as follow: Group 1 = 4.15 MPa; Group 2 = 3.00 MPa; Group 3 = 4.45 MPa; Group 4 = 6.96 MPa; and Group 5 = 8.93 MPa. These values were analysed using one-way ANOVA and Tukey honestly significant difference test (P<.05). Significantly higher bond strength values were obtained with the diode laser and orthophosphoric acid (P<.05). There were no differences found between the other groups (P>.05). CONCLUSION. Orthophosphoric acid and EDTA were more effective methods for removing the smear layer than the diode laser. However, the diode laser and orthophosphoric acid were more effective at the cement dentin interface than the EDTA, Therefore, modifying the smear layer may be more effective when a self-adhesive system is used.Öğe Effect of Different Final Irrigation Methods on the Removal of Calcium Hydroxide from an Artificial Standardized Groove in the Apical Third of Root Canals(ELSEVIER SCIENCE INC, 2014) Capan, Ismail Davut; Ozcan, Erhan; Arslan, Hakan; Ertas, Huseyin; Aydinbelge, Hale AnIntroduction: The aim of this study was to compare the efficacy of conventional syringe, ultrasonic, EndoVac (Discus Dental, Culver City, CA), and Self-Adjusting File (SAF) (Re-Dent-Nova, Ra'nana, Israel) irrigation systems in removing calcium hydroxide (Ca[OH](2)) from simulated root canal irregularities. Methods: The root canals of 88 extracted single-rooted teeth were prepared using ProTaper rotary instruments (Dentsply Maillefer, Ballagiues, Switzerland) up to size F4. The roots were split longitudinally, and a standardized groove was prepared in the apical part of 1 segment. The root halves were reassembled, and Ca(OH)(2) medicament was placed into the root canals using a Lentulo spiral. The roots were randomly divided into 4 experimental groups and 2 control groups according to the different irrigation systems used: conventional syringe irrigation, continuous passive ultrasonic irrigation (PUI), EndoVac irrigation, and SAF irrigation. Each group was then divided into 2 subgroups (n = 10) according to the irrigation protocol: subgroup 1: 10 mL 2.5% NaOCl and subgroup 2: 10 mL 17% EDTA + 10 nnL 2.5% NaOCl. The amount of remaining medicament was evaluated under a stereomicroscope at 30x magnification using a 4-grade scoring system. The influences of the different Ca(OH)(2) medicament removal methods and irrigation protocols were statistically evaluated using 2-way analysis of variance and Tukey post hoc tests. Results: In the NaOCl-irrigated groups, PUI removed significantly more Ca(OH)(2) medicament than the other techniques (P <.05). There was no significant difference among the other groups (P>.05). In the EDTA/NaOCl-irrigated groups, the SAF and PUI removed significantly more Ca(OH)(2) than the other techniques (P <.05). Conclusions: The use of the SAF system with the combination of EDTA and NaOCl enhanced Ca(OH)(2) removal when compared with the use of only NaOCl irrigation with the SAF. Continuous PUI and SAF were more effective than EndoVac, and conventional syringe irrigation in the removal of the Ca(OH)(2) medicament from an artificial Standardized groove in the apical part of the root canal.Öğe The effect of luting cement thicknesses on the push-out bond strength of the fiber posts(TAYLOR & FRANCIS LTD, 2013) Ozcan, Erhan; Cetin, Ali Riza; Tuncdemir, Ali Riza; Ulker, MustafaObjective. The aim of this study was to evaluate the effect of luting cement thicknesses on the push-out bond strength of the fiber posts. Materials and methods. Sixty human maxillary canine teeth were endodontically treated. Post spaces were prepared and root specimens randomly divided into three groups: Group 1: etch-and-rinse (Variolink II/Exite DSC), Group 2: self-etch (Panavia F2.0/ED primer) and Group 3: self-adhesive (Clearfil SA Cement). Then each group was divided into four sub-groups according to the cement thickness, as follows: Sub-group 1: 0.35 mm, Sub-group 2: 0.25 mm, Sub-group 3: 0.15 mm, and Sub-group 4: 0.05 mm. Three slices of 1 mm thickness were obtained from each root specimen (n = 15). Push-out tests were performed and data was analyzed with two-way ANOVA and Tukey tests. Results. The etch-and-rinse system (Variolink II) had a significantly higher bond strength compared with the other systems (p < 0.05). The thickest cement group (0.35 mm) had a significantly lower bond strength compared with the 0.15 and 0.05 mm groups in the etch-and-rinse and self-adhesive groups (p < 0.05). In the self-etch group, the cement thicknesses did not significantly affect the bond strength (p > 0.05). Conclusions. The etch-and-rinse system offered better bonding to root dentine than the self-adhesive and self-etch systems. The increases in cement thickness significantly reduced the bond strength of fiber posts to root dentine for both the etch-and-rinse and self-adhesive systems.Öğe Influence of eugenol on the push-out bond strengths of fiber posts cemented with different types of resin luting agents(SPRINGER, 2013) Ozcan, Erhan; Cetin, Ali Riza; Capar, Ismail Davut; Tuncdemir, Ali Riza; Aydinbelge, Hale AriThis study evaluated the influence of eugenol on the push-out bond strengths of fiber posts cemented with different types of resin luting agents. Seventy-two extracted maxillary single-rooted canine teeth were randomly divided into two groups of 36 teeth. Group 1, the control group, was filled with gutta-percha only (i.e., did not receive eugenol), whereas group 2 was filled with a eugenol-containing sealer. All root canals were filled and each group was divided into three subgroups. The posts in each subgroup were cemented with the following materials: subgroup 1 with a 2-step self-etching adhesive system (Clearfil Liner Bond 2V + Panavia F); subgroup 2 with a 1-step self-etching adhesive (Panavia F); and subgroup 3 with a self-adhesive (Clearfil SA Cement). Dislodgement resistance was measured using a universal testing machine. All data were subjected to ANOVA using a factorial design and Tukey test (alpha = 0.05). The use of the eugenol-containing sealer significantly reduced the push-out bond strength of the fiber post (P < 0.05). The push-out bond strength of Panavia F was significantly higher than those of the other groups filled with the eugenol-containing sealer (P < 0.05). The Panavia F group was less susceptible to the inhibiting effect of eugenol than were the other evaluated groups when the fiber post was cemented in the canals filled with the eugenol-containing sealer.
