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Öğe A Comparison of Immuncapture Agglutination and ELISA Methods in Sero-logical Diagnosis of Brucellosis(IVYSPRING INT PUBL, 2011) Ozdemir, Mehmet; Feyzioglu, Bahadir; Kurtoglu, Muhammed Guzel; Dogan, Metin; Dagi, Hatice Turk; Yuksekkaya, Serife; Kesli, RecepBackground: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and im-muncapture agglutination tests with Coombs anti-brucella test. Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were in-cluded into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Im-muncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test. Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.Öğe The effect of physical activity on level of anger among individuals with autism(2018) Ilkım, Mehmet; Tanır, Halil; Ozdemir, Mehmet; Bozkurt, İbrahimThis study was undertaken to investigate the effect of physical activity on anger level in autistic individuals. Study sample included parents of 40 autistic children (n20 experimental and n20 control) aged 10-14 years from Malatya Private Sevgi Yolu Rehabilitation Center. Three days a week for 16 weeks, the experimental group did the activities of one-hour walk, jogging, double-leg jump, and walking along a balancing beam. Individuals in the control group continued their daily routine without participating in any activity during this period. Anger levels of autistic individuals were identified using the Trait Anger and Anger Expression Inventory (TAAEI) developed by Spielberger et al. (1983) and adapted to Turkish by Özer (1994). The inventory was completed by the parents of the individuals both in the experimental and control groups following the 16-week activity program. The collected data was analyzed at the significance level of 0.05 (i.e. a confidence level of 95%) in SPSS version 22.0. Statistical analysis indicated significant differences between trait anger, controlled anger, expressed anger and suppressed anger scores (p0.05). The results showed that anger levels of the autistic individuals participating in the physical activity program were lower and the physical activity in autistic individuals helped anger control. It is recommended that autistic individuals participate in low intensity activities for at least 3 days a week. By teaching autistic individuals how to keep their anger under control, interpersonal and family conflicts, feelings of guilt, low self-esteem and some behavioral (e.g. shyness, crying, unhappiness, anorexia, bulimia) and physiological problems (e.g. sweating, headache, difficulty in breathing) can be avoided.Öğe Isolation of citrobacters in various infections and their antimicrobial sensitivity rates(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2011) Kurtoglu, Muhammet Guzel; Opus, Aysegul; Ozdemir, Mehmet; Baysal, BulentThe samples for culture from the patients admitted to Konya Research and Education Hospital between January 2009 and July 2010 were investigated. The obtained Citrobacter strains were studied for antimicrobial sensitivity rates and their distribution ratesto the clinics. The samples obtained were inoculated onto related culture media. Aseptic body samples were incubated at 37 degrees C into bottles of BACTEC 9120 blood culture system. For the identification and antimicrobial sensitivities of yielding bacteria, panels of Phoenix-100 automized identification device were used. Mean age rate of patients in whom Citrobacter strains were determined was 41.29 +/- 4.345. Of all samples with Citrobacter strains, 48% were isolated from urine, 29% from surgical wounds, 11% from sputum, 2% from peritoneal fluid, and 2% from vaginal samples. Of total 52 Citrobacter strains, the species level distribution was 46% C. freundii, 21% C. youngae, 15% C. koseri, 10% C. braakii, 6% C. farmeri and 2% C. wermanii. The distribution of samples with Citrobacter strains to the clinics were 29% adult intensive care unit (ICU), 21% pediatric, 11% general surgery, 10% in neonatal ICU, 10% plastic surgery, 7% urology, 6% burn unit, and 6% nephrology department. The most sensitive antimicrobial agents to Citobacter strains were amikacin (100%), meropenem (100%), imipenem (96%) and piperacillin/tazobactam (96%).Öğe Isolation Rate of Mycobacterium tuberculosis Complex from Patients with Suspected Tuberculosis and Identification of the Strains with BACTEC (TM) NAP and Immunochromatographic TB Ag MPT64 Rapid (TM) Tests(ANKARA MICROBIOLOGY SOC, 2011) Kurtoglu, Muhammet Guzel; Ozdemir, Mehmet; Kesli, Recep; Ozkalp, Birol; Baysal, BulentTuberculosis (TB) which is still one of the important infectious diseases in the world as well as Turkey, results in high morbidity and mortality. Clinical mycobacteriology laboratories have crucial roles in the identification, typing and susceptibility testing of Mycobacterium tuberculosis. The aims of this study were the investigation of the isolation rate of M.tuberculosis complex (MTC) from the clinical specimens of TB-suspected patients and to compare identification of mycobacteria isolated from solid and/or liquid media by using BACTEC NAP and immunochromatographic TB Ag MPT64 rapid test. A total of 1670 patients who were admitted to outpatients clinics of our hospital and prediagnosed as TB, have been included in the study. All the patients were anti-HIV seronegative. NALC-NaOH method were used for decontamination/homogenization, and preparations from samples were stained with Erlich-Ziehl-Neelsen method to detect acid-resistant bacilli (ARB) in direct microscopy. All of the samples were inoculated into BACTEC (TM) MGIT-960 (Becton Dickinson, USA) and Lowenstein-Jensen (14) media for cultivation and incubated at 37 degrees C for 6-8 weeks. Mycobacteria that were grown in the media have been identified by BACTEC (TM) NAP (Becton Dickinson, USA) and TB Ag MPT64 rapid test (SD Bioline Ag MPT64 Rapid (TM); Standard Diagnostics, Korea). The culture positivity in the samples of TB-suspected patients was found to be 3.7% (63/1670) with LJ and/or MGIT-960 methods, whereas ARB positivity rate was 1.6% (28/1670). Fifty-three (84%) out of culture positive 63 samples have been identified as MTC by BACTEC NAP test, while 61(97%) were found as MTC by TB MPT64 test. Considering BACTEC NAP test as the reference method, TB MPT64 test identified all the MTC strains correctly (sensitivity: 100%), however the false positivity rate was estimated as 12.7% (specificity: 87%). Of 53 MTC positive samples, 36 were sputum, four were bronchoalveolar lavage, four were urine, three were gastric fluid, three were pleural fluid, and one of each were abscess, peritoneal fluid and cerebrospinal fluid samples. ARB positivity rate was detected as 41.5% (22/53) among MTC culture positive samples. Of the patients who were infected with MTC, 72% (38/53) were male and 98% (52/53) were adults (age range: 20-85 years). Our data indicating 3.1% (53/1670) isolation rate of MTC from TB-suspected patients in our region were in concordance with the other results reported from Turkey. In conclusion, immunochromatographic TB Ag MPT64 test which seemed to be useful for the rapid identification of mycobacteria grown on solid and/or liquid, was practical to perform and had high sensitivity, however further larger-scaled studies are needed to support our data in our country.Öğe Laboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodies(GEORG THIEME VERLAG KG, 2018) Feyzioglu, Bahadir; Yavru, Sibel; Ozdemir, MehmetIntroduction Central nervous system (CNS) viral infections are a serious problem requiring accurate diagnosis and treatment. Human herpesviruses (HHVs) are an important cause of these infections. Recent research has focused on new diagnostic methods allowing accurate and rapid identification of viral infections because there are still diagnostic difficulties for these infections. This study was done to determine the etiologic role of human herpes viruses and to obtain information that will contribute to the diagnostic algorithm in suspected cases of viral encephalitis or aseptic meningitis. Materials and Methods In our study, herpes simplex virus (HSV)-1, HSV-2, varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and HHV-6 DNA was detected in the cerebrospinal fluid (CSF) by multiplex polymerase chain reaction (PCR) and virus-specific immunoglobulin G (IgG) antibodies in CSF and serum by EIA in pediatric encephalitis/meningitis cases. Results HSV-1 and VZV were detected in 5 and 3.3% of aseptic meningitis cases, respectively, but no encephalitis cases. Other viruses were not identified as etiologic agents. The seroprevalences were determined as 72.4, 34.3, 81.9, 93.3, 88.6, and 80.9%, respectively for HSV-1, HSV-2, VZV, CMV, EBV, and HHV-6. The performance of specific IgG CSF/serum antibody index (AI) was not satisfactory. Conclusion Our study indicates that the multiplex PCR method is the most suitable for the diagnosis of CNS infections caused by HHVs. However, due to the high cost of the PCR method, the positive results of the specific AI may be significant, but the negative results are unreliable, especially in limited health care facilities.Öğe Nasal carriage of Staphylococcus aureus in 4-6 age groups in healthy children in Afyonkarahisar, Turkey(WILEY, 2007) Ciftci, Ihsan Hakki; Koken, Resit; Bukulmez, Aysegul; Ozdemir, Mehmet; Safak, Birol; Cetinkaya, ZaferAim: Staphylococcus aureus is a common cause of disease, particularly for colonized persons. Although methicillin-resistant S. aureus (MRSA) infection has frequently reported, population-based S. aureus and MRSA colonisation estimates are lacking. Our objective in this report is to present the prevalance of S. aureus carriage among 4-6 age groups healthy children in our region. Methods: Nasal samples for S. aureus culture were obtained from healthy children. Sociodemographic features and the data related with risk factors were obtained from the parents of the children. Nasal swabs were inoculated on to a variety of bacteriological culture media, which were then incubated at 35 +/- 1 degrees C for 16-18 h. Antimicrobial susceptibility testing of the isolates was determined according to Clinical and Laboratory Standard Institute (CLSI, 2005) guidelines. Results: In this study 1134 children between 4 and 6 years old age were evaluated; 607 (53.5%) of the subjects were boys and 527 (46.5%) were girls. S. aureus was isolated in 322 (28.4%) subjects and MRSA was isolated in 3 (0.3%) subjects of them. All of the MRSA isolates were found in Conclusions: This first assessment of this study is that nearly one third of the 4-6 age group healthy children population present nasal carriage of S. aureus in Turkey. However, it also shows that the rate of MRSA carriage remains low. in addition, it is considered that MRSA colonization may be a risk factor for healthcare workers' children.Öğe Ocular Surface Disorders in Intensive Care Unit Patients(HINDAWI PUBLISHING CORPORATION, 2013) Saritas, Tuba Berra; Bozkurt, Banu; Simsek, Baris; Cakmak, Zeynep; Ozdemir, Mehmet; Yosunkaya, AlperPatients in intensive care units (ICU) are at increased risk of corneal abrasions and infectious keratitis due to poor eyelid closure, decreased blink reflex, and increased exposure to pathogenic microorganisms. The aim of this retrospective study was to evaluate the ocular surface problems in patients who stayed in ICU more than 7 days and were consulted by an ophthalmologist. There were 26 men and 14 women with a mean age of 40.1 +/- 18.15 years (range 17-74 years). Conjunctiva hyperemia, mucopurulent or purulent secretion, corneal staining, and corneal filaments were observed in 56.25%, 36.25%, 15%, and 5% of the eyes, respectively. Keratitis was observed in 4 patients (10%) who were treated successfully with topical antibiotics. Mean Schirmers test results were 7.6 +/- 5.7 mm/5 min (median 6.5 mm/5 min) in the right, and 7.9 +/- 6.3 mm/5 min (median 7 mm/5 min) in the left eyes. Schirmers test results were <5 mm/5 min in 40% of the subjects. The parameters did not show statistically significant difference according to mechanical ventilation, sedation, and use of inotropes. As ICU patients are more susceptible to develop dry eye, keratopathy, and ocular infections, they should be consulted by an ophthalmologist for early diagnosis of ocular surface disorders.Öğe A Rare Cause of Bacteremia in a Pediatric Patient with Down Syndrome: Sphingomonas Paucimobilis(IVYSPRING INT PUBL, 2011) Ozdemir, Mehmet; Pekcan, Sevgi; Demircili, Mehmet Emin; Tasbent, Fatma Esenkaya; Feyzioglu, Bahadir; Pirinc, Serife; Baykan, MahmutSphingomonas paucimobilis, is a yellow-pigmented, aerobic, non fermentative, gram negative motile bacillus. S. paucimobilis which is widely found in nature and hospital environments rarely cause serious or life threatening infections. In this report, a case of hospital acquired bloodstream infection due to S. paucimobilis in a patient with Down syndrome who was on treatment for presumed pneumonia is presented. A one year-old child patient who was a known case of Down syndrome and had previously experienced cardiac surgery was hospitalized and treated for pneumonia. On the 12th day of hospitalization, blood cultures were taken because of a high body temperature. One of the blood cultures was positive for gram-negative rods. After 48 hour of incubation, the sub-cultures on blood agar medium yielded pure growth of a yellow, non-fermentative, gram-negative, rod-shaped bacterium. The microorganism was positive for oxidase, and esculin hydrolysis, while negative for urea and nitrate reduction, citrate utilisation and motility. The isolate had been identified as S. paucimobilis by using Vitek 2 system. The antibiotic susceptibility test was also performed with the same system and the strain was found to be susceptible to piperacillin-tazobactam and other antibiotics. Treatment with intravenous piperacilin-tazobactam (150 mg/kg/day) was initiated. He responded well to the treatment and was discharged after 10 days. This case is reported to emphasize that S. paucimobilis should be kept in mind as a nosocomial infectious agent in patients with Down syndrome and immunosuppressive patients and the infections should be treated according to the sensitivity test results.Öğe A research on professional anxiety levels of 3rd and 4th grade students of faculty of sport sciences of Selçuk University(2018) Topuz, Ramazan; Ozdemir, Mehmet; Bozkurt, İbrahimThe aim of this research is to determine the level of professional anxiety of the teacher candidates who are studying in the departments of the Faculty of Sports Sciences, is the prediction of starting teaching profession and continuing their profession; is to examine the anxieties of the profession in terms of various variables. The universe of research; Selcuk University, and the sample is constituted.by 88 female, 163 male, total 251 students who are studying at Sports Sciences Faculty 3rd and 4rd grade of Selcuk University in 2017-2018 education period,In the research,the eight sub-dimensions which are the professional anxiety sub-dimensions;task-centered anxiety, economic / social centered anxiety, student / communication centered anxiety, colleague and parent centered anxiety, personal development centered anxiety, assignment centered anxiety, integration centered anxiety, school management centered anxiety are evaluated according to socio-demographic characteristics applying the Vocational Anxiety Inventory and demographic information form containing the questions determined by the researchers as the data collection tool. (6). It has been checked that if the data show normal distribution by Kurtosis – Skewness coefficent interval, it was found that the data were distributed normally since the subject interval did not exceed the values of 2.0 and - 2.0. (12). Independent group t test was used for binary cluster comparisons since the data showed normal distribution. For this study, the Cronbach Alpha coefficent was calculated as 0.88. Significance level in the study was taken as 0.05. At the result of the research, no statistically significant difference was observed in all the sub-dimensions of the students who participated in the research, according to the educational level of the parents, age and gender. As a result of examining according to the class level variable; While no statistically significant difference was observed between the Economic / Social Centered Anxiety, Student Communication Centered Anxiety, Personal Development Centered Anxiety, Integration Centered Anxiety and School Management Centered Anxiety sub-dimensions, It was determined that the anxieties of students with 4th grade education level were higher than those of 3rd grade students in colleague and parent-centered anxiety and assignment-centered anxiety dimensions.Öğe Side effect of metenolone enanthate on rats heart in puberty: Morphometrical study(ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2013) Ozdemir, Ozgur; Bozkurt, Ibrahim; Ozdemir, Mehmet; Yavuz, OrhanThe aim of this study was the investigation of effects of the metenolone enanthate (ME) that is used among athletes as doping and muscle amplifier, on hearts of male and female rats that are in puberty using morphometrical methods. A total of 36 rats which were divided into three separate groups (Experiment, ME; vehicle, PO; control, C) each consisting of 6 male and 6 female rats were used. 0.5 mg/kg metenolone enanthate was applied intraperitoneally into experiment subjects 5 times a week over a period of 4 weeks. At the end of experiment, rats were euthanized and their hearts were cut at the level of musculus papillaris after the fixation in formalin. Hearts were taken out and embedded in paraffin wax. Photos were taken at cut surfaces, and thickness, diameters and surface area levels were measured. Left ventriculus mass (LVM) and left ventriculus mass index (LVMI) were calculated. In the study LVM (p < 0.005) and LVMI (p < 0.05) were found to be significantly higher in the ME group in females whereas left ventricular lumen diameter (LVLD) were found to be significantly lower (p < 0.05). Thus left ventricular hypertrophy development was observed. LVM and LVMI were found to be similar in ME and C groups among male rats and the highest level of these data were found in the group. LVM and LVMI were higher among females (p < 0.006). In conclusion, it has been shown that the adverse effects of ME on heart were developing starting from puberty and resulting with the enlargement of the heart and left ventricular hypertrophy and especially among females this condition was more evident. It has also been discussed that the continuous use of drugs may further enhance this condition. (c) 2012 Elsevier GmbH. All rights reserved.Öğe Sports injuries in 15-17 year-old male athlete students participating on school teams(2018) Ozdemir, Mehmet; Tanır, Halil; Ilkım, Mehmet; Şeker, TarıkThe purpose of this study is to determine the frequency of the sports injuries in 15-17-year-old male athlete students participating on school teams (volleyball, basketball, football and handball) and their level of knowledge about these injuries. The sample of the research consists of 233 athlete students (n89 footballers, n37 volleyball players, n46 basketball players and n61 handball players) in 15–17 age group participating on school teams of thirteen different high schools in Kütahya. The questionnaire form developed was used to determine the injuries in athlete students that participated in the study. The data was analysed using SPSS version 22.0. According to the statistical analysis, it was found that the athlete students who got injured most were of footballers with %45.2 (n47) and those who got injured least were volleyball players with %10.6 (n11). It was seen that injuries occurred mostly during competitions (n82). The majority of the athlete students reported that they had very little (n117) and average (n107) information on sports injuries. The results showed that injuries occurred more in football and handball players and mostly during competitions. Protective measures need to be taken in the sports, especially in football, handball and basketball, where tackles are frequently experienced during competitions. Also, it was seen that athlete students had very limited knowledge about sports injuries.
