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Öğe DGAT1-exon8 polymorphism in Anatolian buffalo(ELSEVIER SCIENCE BV, 2012) Ozdil, Fulya; Ilhan, FatmaPrevious studies have reported that a non-conservative substitution of lysine by alanine (K232A) in the 8th exon of acyl CoA:diacylglycerol acyltransferase (DGAT1) gene in cattle has a major effect on milk composition and yield. But yet little research has been utilized in this gene segment in buffalo. In this study the genetic differentiation of three indigenous Anatolian buffalo populations has been investigated in the 8th exon of DGAT1 gene. Fourteen out of the 24 restriction enzymes have a recognition site on the DGAT1 gene segment, generating a total of 26 restriction sites. AluI, HincII and HphI restriction enzymes out of these 14 enzymes were found to detect polymorphism. K232A substitution found in cattle breeds with CfrI restriction enzyme is not a diagnostic site in Anatolian buffalo. Only lysine variant (K allele) is found in all of the Anatolian buffalo tested. In this study, four different haplotypes were obtained and sequencing of this gene revealed three polymorphic nucleotide substitutions in the 8th exon of the buffalo DGAT1 gene. C -> T, A -> G and C -> G base substitutions at positions 43, 154 and 373, respectively, each generated two different fragment patterns in Anatolian buffalo. Type 1 haplotype was the most common haplotype, found in 73.2% of the samples. Along with the polymorphic substitution sites in DGAT1 gene, this study provides evidence that all the Anatolian buffalo have fixed allele with respect to DGAT1 K allele reported to be responsible for high milk fat yield. Our study presents the first comprehensive sequencing analysis of Anatolian buffalo and it is the first time that sequencing data from DGAT1 gene segment have been obtained at the population level. (C) 2012 Elsevier B.V. All rights reserved.Öğe Diversity of Apis mellifera Subspecies from Turkey Revealed by Sequence Analysis of Mitochondrial 16s rDNA Region(SPRINGER/PLENUM PUBLISHERS, 2012) Ozdil, Fulya; Ilhan, FatmaMitochondrial DNA sequence variation can be used to infer honeybee evolutionary relationships. In this study, DNA sequence diversity of the mitochondrial 16s rDNA region was investigated in 112 honeybees from 15 populations in Turkey, which is mainly populated with Apis mellifera anatoliaca, A. m. caucasica, and A. m. meda. The study revealed 11 haplotypes for this segment, with 13 variable sites and nine parsimony informative sites. The haplotypes were not discriminated according to their geographical locations in a neighbor-joining dendrogram based on 16s rDNA sequences available in Genbank, but all the haplotypes obtained in this study are clustered with published haplotypes such as A. mellifera TAS (AF214666) and A. m. ligustica (EF116868) and with some unpublished Genbank records (HQ318928, HQ318934, and HQ318938). This study expands the knowledge of the mitochondrial 16s rDNA region, and it presents the first comprehensive sequence analysis of this region in Turkish honeybees.Öğe Genetic characterization of some Turkish sheep breeds based on the sequencing of the Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region(COPERNICUS GESELLSCHAFT MBH, 2018) Ozdil, Fulya; Ilhan, Fatma; Isik, RaziyeIn this research, Ovar-DRB1 gene in the major histocompatibility complex (MHC) gene region was surveyed by DNA sequencing in some of the native sheep breeds that are reared in Turkey. A total of 80 samples were collected from eight different Turkish native sheep breeds, and these samples were used for DNA sequencing. The exon 2 region of Ovar-DRB1 in the MHC gene region was polymerase chain reaction (PCR) amplified and sequenced. A total of 25 new alleles were revealed in the Ovar-DRB1 gene in Turkish native sheep breeds with 24 variable sites; only 13 sites were parsimony informative. The average pairwise genetic distance was 0.029 % for the Ovar-DRBI gene exon 2 region. The sequence variations at eight different positions (7026, 7036, 7040, 7053, 7059, 7069, 7131 and 7214) are found in all of the studied samples. G -> C transversion at position 7081 is only seen in Akkaraman sheep breed, whereas T -> C transition at position 7097 is only seen in one sample from the Akkaraman breed. Overall, two main groups were detected among the 25 alleles from Turkish native sheep breeds. All Daglic and Kivircik alleles and one allele from Karayaka, Malya and Sakiz are grouped together while all the other breeds are grouped in the other branch.Öğe Genetic Diversity of Turkish Honey Bee Populations Based on RFLPs at a Nuclear DNA Locus(CALIFORNIA STATE UNIV, 2011) Ozdil, Fulya; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. GlennGenetic diversity of Turkish honey bee populations was examined by PCR-RFLP of an anonymous nuclear DNA locus named 1231 region. The two halves of this locus, E(1)N and NE(2), were amplified from 70 bee colonies from 9 different localities. The restriction site differences responsible for the allelic RFLP patterns were determined. In one half of the 1231 region, E(1)N, was digested with BclI, HhaI and XhoI. The other half of this 1231 region, NE(2), was digested with AluI, NsiI, PstI, XhoI, HhaI and HindIII. One to seven different restriction patterns for each restriction enzyme were found in Turkish honey bees and reference samples, European and African honey bees.Öğe Identification of factor XI deficiency in Holstein cattle in Turkey(BIOMED CENTRAL LTD, 2009) Meydan, Hasan; Yildiz, Mehmet A.; Ozdil, Fulya; Gedik, Yasemin; Ozbeyaz, CeyhanBackground: Factor XI (FXI) is a plasma protein that participates in the formation of blood clots. Factor XI deficiency is autosomal recessive hereditary disorder that may be associated with excess bleeding in Holstein cattle. Methods: In this study, 225 Holstein cows reared in Turkey were screened in order to identify FXI genotypes. DNA extractions were obtained from the fresh blood of the cows. Amplicons of FXI exon 12 were obtained by Polymerase Chain Reaction (PCR), and analyzed by 2% agarose gel electrophoresis stained with ethidium bromide. Additionally, all cows were confirmed by DNA sequencing to determine whether or not there was a mutant allele. Results: Carriers of the FXI deficiency have two DNA fragments of 320 bp and 244 bp in size. The results of our study demonstrated that only four out of the 225 Holstein cows tested in Turkey carried the FXI deficiency. The frequency of the mutant FXI allele and the prevalence of heterozygous cows were found as 0.9% and 1.8%, respectively. Conclusion: The DNA-based test determines all genotypes, regardless of phenotype or FXI activity. The mutation responsible for the FXI deficiency had not been detected in Holstein cattle in Turkey before prior to this study. The frequency of the mutant FXI allele needs to be confirmed by carrying out further analyses on cattle in Turkey and the selection programs should be developed to eliminate this genetic disorder.Öğe Mitochondrial DNA Variation in the CoxI-CoxII Intergenic Region among Turkish and Iranian Honey Bees (Apis mellifera L.)(SPRINGER/PLENUM PUBLISHERS, 2009) Ozdil, Fulya; Fakhri, Bahman; Meydan, Hasan; Yildiz, Mehmet Ali; Hall, H. Glenn[Abstract not Available]Öğe Molecular characterization of Turkish honey bee populations (Apis mellifera) inferred from mitochondrial DNA RFLP and sequence results(EDP SCIENCES S A, 2009) Ozdil, Fulya; Yildiz, Mehmet Ali; Hall, H. GlennTo identify the evolutionary lineage of honey bee colonies in Turkey, the mtDNA of 244 colonies from 20 locations was analyzed. Several polymorphic restriction sites showed that they belonged to the Mediterranean C lineage. DraI digestion of the CoxI-CoxII intergenic region produced four fragment patterns, one first seen in this study. From 37 colonies from 16 different locations in Turkey and two colonies from Iran, the intergenic region was sequenced. Previously, from among all honey bee populations of the C lineage, eight haplotypes had been described from this mtDNA region, three of which were found here. In addition, eight new haplotypes were found. A deletion in one of these haplotypes accounts for the novel DraI RFLP pattern. Most of the novel haplotypes were in a subgroup of lineage C, yet none of these had been found in previous studies of Turkish honey bees. The geographical distribution of some haplotypes suggests that they may be characteristic of subspecies native to Turkey.
