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Öğe Investigation of enzootic bovine leucosis (ebl) infection by clinical, serological, haematological and pathological examinations in the three dairy cows(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2007) Kale, Mehmet; Ozmen, Ozlem; Sahinduran, Sima; Yavru, SibelIn this study, Enzootic Bovine Leucosis (EBL) infection in three Holstein breed cows, three years old, were investigated interms of clinical, serological, hematological and pathological examination. Clinical examinations revealed weakness, lack of appetite, enlargements in all superficial lymph nodes especially marked in prescapular lymph nodes, high temperature, cardiac arrhythmia, respiratory disturbance and incoordination. Serologically, Bovine Leucemia Virus (BLV) antibodies were detected in blood serum samples by Agar-Gel Immunodiffusion (AGID) and Enzyme Linked Immunosorbent Assay (ELISA). Hematologically, increases were found in B-lymphocytes, total leucocytes, total lymphocyte and T-lymphocytes, but null cells and neutrophil ratios decreased in all three cows. Lymphadenopathy was observed in all lymph nodes at the pathological examination after slaughter. Especially at the superficial lymph nodes cortex and medulla were irregular and hemorrhages were observed.Öğe Pathological, serological, and virological findings in sheep infected simultaneously with Bluetongue, Peste-des-petits-ruminants, and Sheeppox viruses(SPRINGER, 2009) Ozmen, Ozlem; Kale, Mehmet; Haligur, Mehmet; Yavru, SibelIn this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep and lambs that suffer from simultaneous naturally occurring BTV, PPRV and SPV outbreaks. SPV was diagnosed macroscopically and histopathologically, BTV was diagnosed by ELISA, and PPRV was diagnosed pathologically and by ELISA. Clinically fever, diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia, cutaneous eruption with erythematous areas especially noticeable in wool-free parts of the body and axilla lesions evolving into papules were observed. At necropsy, the most effected organs were lungs and gut. Subepicardial hemorrhages were also commonly seen. While typical pox lesions were observed in some lambs, usually fibrinous pleuropneumonia was more prominent lung lesion. SPV and PPRV lesions were seen at the histopathological examination of the lesioned tissues, BT lesions were mild than SPV and PPRV microscopically. Serum and leukocyte samples of 15 animals were examined for PPRV and BTV by ELISA; 5 samples were positive for PPRV and 6 BTV, 4 were positive for both PPRV and BTV simultaneously. One hundred animals died, most were lambs. Mortality rates were 100% in lambs and 80% in the herd.