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Öğe Bacillus anthracis Isolated from a Dog in Turkey(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2015) Sayin, Zafer; OzgurOzdemir; Erganis, Osman; Hadimli, Hasan Huseyin; Sakmanoglu, Asli; Atil, Eray; Sanioglu, GokcenurAn Anatolian Akbash shepherd dog died suddenly, without any clinical signs in Konya, Turkey. Performing necropsy, pathological examination, a culture test, laboratory tests and a multiplex-PCR of bacteria isolated from the dog revealed an anthrax and identified the bacteria as Bacillus anthracis (B. anthracis). The chromosomal gene sequence of bacteria was 99% identical in the GenBank under accession numbers CP002091 (B. anthracis str. H9401), CP001598 (B. anthracis str. A0248) and CP001215 (B. anthracis str. CDC684). Antimicrobial susceptibility test was performed and cefuroxime, cefquinome, sulfamethoxazole-trimethoprim, chloramphenicol, tetracycline and rifampicin resistance were seen in isolate. In this case, how the dog was infected with B. anthracis could not be determined. (C) 2014 PVJ. All rights reservedÖğe Determination of effectiveness of IgY and bacteriophage therapy against Salmonella infections in calves(ELSEVIER SCIENCE BV, 2016) Hadimli, Hasan Huseyin; Sanioglu, Gokcenur; Gurbuz, Eda; Uslu, Ali; Ilban, Aysegul; Cokurgen, Tunca[Abstract not Available]Öğe Genotypic Characterization of Bordetella bronchiseptica Strains Isolated from Stray and Pet Dogs(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2016) Sayin, Zafer; Sakmanoglu, Asli; Erganis, Osman; Ucan, Uckun Sait; Hadimli, Hasan Huseyin; Aras, Zeki; Sanioglu, GokcenurBordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. (C)2016 PVJ. All rights reservedÖğe Serologic prevalence of Ornithobacterium rhinotracheale infection in commercial layers(2016) Aras, Zeki; Sayin, Zafer; Sanioglu, Gokcenur. Amaç: Bu çalışmada ticari yumurtacı tavuk işletmelerinde bulunan tavuklarda Ornithobacterium (O.) rhinotracheale enfeksiyonunun serolojik prevalansının belirlenmesi amaçlandı.Gereç ve Yöntem: Konya, Aksaray, Karaman, Ankara ve Gaziantep illerinde bulunan 26 farklı kümesteki yumurtacı tavuklardan 650 kan serum örneği toplandı. Örnekleme yapılan tavukların hiç birisi daha önceden O. rhinotracheale enfeksiyonuna karşı aşılanmamışlardı. O. rhinotracheale'ye karşı oluşmuş antikorların varlığı ELISA testi ile belirlendi.Bulgular: Toplam 650 kan serum örneğinden 113 (%17.4)'ünde O. rhinotracheale antikoru tespit edildi. Bu 113 pozitif serum örneği örnekleme yapılan 26 kümesin 12 (%46.2)'sinden toplanmıştı. Enfekte 12 kümes, Konya, Gaziantep, Ankara ve Karaman illerindeki 12 farklı çiftliğe aitti. Öneriler: Bu çalışmanın sonuçları göstermiştir ki, Türkiye'deki ticari yumurtacı tavuk kümeslerinde O. rhinotracheale enfeksiyonu yüksek bir prevalansa sahiptir.Öğe Serologic prevalence of Ornithobacterium rhinotracheale infection in commercial layers(Selçuk Üniversitesi Veterinerlik Fakültesi, 2016) Aras, Zeki; Sanioglu, GokcenurAim: The purpose of this study was to determine the serological prevalence of Ornithobacterium (O.) rhinotracheale infections in commercial layers. Materials and Methods: A total of 650 blood serum samples were collected from 26 different layer flocks located in Konya, Aksaray, Karaman, Ankara, and Gaziantep provinces. None of the chickens have been vaccinated against O. rhinotracheale prior to sampling. The presence of antibodies against O. rhinotracheale in each sample was determined by ELISA. Results: Of the 650 serum samples, 113 (17.4%) were found to be positive for O. rhinotracheale antibodies. These 113 positive sera were collected from 12 (46.2%) out of 26 flocks. The twelve infected flocks belonged to 12 different farms form Konya, Gaziantep, Ankara and Karaman. Conclusion: The results of this study indicated that O. rhinotracheale infection is at high prevalence in the commercial layer flocks from this part of Turkey
