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  • Küçük Resim Yok
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    Expression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterus
    (ELSEVIER SCIENCE INC, 2015) Ağaoğlu, Özgecan Korkmaz; Ağaoğlu, Ali Reha; Güzeloğlu, Aydın; Kurar, Ercan; Kayış, Seyit Ali; Özmen, Özlem; Schafer-Somi, Sabine
    Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7,30-45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of Cl and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy. (C) 2015 Elsevier Inc. All rights reserved.
  • Küçük Resim
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    Expression of platelet activating factors and its receptor in cat uterus during early pregnancy
    (2013) Ağaoğlu, Ali Reha; Ağaoğlu, Özgecan Korkmaz; Kurar, Ercan; Güzeloğlu, Aydın; Kayış, Seyit Ali; Atlı, Mehmet Osman; Schafer-Somi, Sabine; Aslan, Selim
    Platelet activating factors (PAF1?, PAF1?, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAF genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy. In conclusion, PAF genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy.
  • Küçük Resim
    Öğe
    Expression of platelet activating factors and its receptor in cat uterus during early pregnancy
    (ANKARA UNIV PRESS, 2013) Ağaoğlu, Ali Reha; Ağaoğlu, Özgecan Korkmaz; Kurar, Ercan; Güzeloğlu, Aydın; Kayış, Seyit Ali; Atlı, Mehmet Osman; Schafer-Somi, Sabine
    Platelet activating factors (PAF1 beta, PAF1 gamma, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAP genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy In conclusion, PAP genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy.