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Öğe Differences in the Somatotropic Axis, in Blood Cortisol, Insulin and Thyroid Hormone Concentrations Between Two Pig Genotypes With Markedly Divergent Growth Rates and the Effects of Growth Hormone Treatment(British Soc Animal Science, 2002) Elsaesser, F.; Pfaffl, M. W.; Meyer, H. H. D.; Serpek, B.; Sauerwein, H.The intention of the current study was to gain more insight into the endocrine and molecular control mechanisms of growth in the pig. For this purpose various growth related parameters were determined in 4-month-old barrows of two extreme pig genotyes, the small, obese Gottingen Miniature (GM) and the large and lean German Landrace (DL). Mean growth hormone (GH) concentration, GH pulse frequency and GH pulse amplitude did not differ between breeds. Likewise, plasma IGF-1, thyroxine, tri-iodothyronine (T) concentrations were similar in both breeds. However the plasma GH response (maximum level and area under curve) to a single i.v. injection of GHRH in DL was higher than in GM (P < 0.05). Furthermore, basal plasma insulin and in particular plasma cortisol concentrations were higher in GM compared with DL pigs (P < 0.05 and <0.01 respectively). Analysis of cortisol during 4-h frequent blood sampling indicated higher cortisol amplitudes in GM compared with DL (P &LE; 0.01). Specific bGH-binding to hepatic membrane preparations was not different between breeds and IGF-1 m RNA concentrations determined by reverse transcription-polymerase chain reaction in liver, m. semimenbranosus and m. longissimus dorsi were similar in both breeds. I.m. treatment with recombinant porcine somatotropin (rpST, 70 μg/kg live weight) over an 8-day period in contemporary barrows increased without any breed difference, plasma IGF-1, T-3 and insulin Concentrations and hepatic specific bGH-binding, but did not affect thyroxine or cortisol concentrations in plasma. IGF-1 gene expression was also elevated in liver and muscle tissues in rpST-treated animals without obvious breed effects. The observations underline the complexity of the hormonal and molecular control of growth and support the notion that differences in growth potential are the consequence of differences at various levels of the somatotropic axis and apparently relate to differences in other control systems of energy metabolism such as the pituitary adrenal axis or the endocrine pancreas as well.Öğe Effect of Training and Gender on Plasma Irisin, Leptin, and Insulin Levels(INT JOURNAL APPLIED EXERCISE PHYSIOLOGY, 2018) Arikan, S.; Revan, S.; Balci, S. S.; Sahin, M.; Serpek, B.The objective of this study is the comparison of the irisin, leptin, and insulin hormone levels of the female and male elite athletes and non-athletes. Elite taekwondo athletes (7 males, 6 females) and university students (8 males, 6 females) between the ages of 16 and 20 years volunteered to participate in this study. In the beginning of the study, the body compositions of the participants were determined and their plasma irisin, leptin, and insulin hormone analyses were determined by the Enzyme-Linked Immuno Sorbent Assay (ELISA) method. After an overnight fast, the blood samples were collected at 8:00 a.m. A two-way ANOVA was performed to examine the effects of gender and training status. There was found no significant main and/or interaction effect of training and gender on the irisin and insulin hormone levels (p>0,05). However, training and gender affected the leptin levels significantly (p<0.05). The leptin levels in females in both athletes and non-athletes were higher than males to significant extent and the leptin levels of both female and male athletes were significantly lower than non-athletes. As a result, it can be said that exercise training status and gender do not affect the levels of irisin and insulin hormones, but they increased the leptin level.Öğe Effects of Dietary Protein Level on Serum Growth Hormone, Insulin-Like Growth Factors and Performance in Kivircik Lambs(MEDWELL ONLINE, 2008) Bilal, T.; Keser, O.; Serpek, B.; Haliloglu, S.Due to studies on the effect of dietary protein on serum Growth Hormone (GH) and insulin-like growth factor-1 (IGF-1) concentrations in lambs fed at different levels of dietary protein are limited, this study was conducted to determine the effect of dietary protein level on GH and IGF-1 concentrations and performance in lambs. A total of 50 Kivircik male lambs were randomly selected for this experiment. Lambs were randomly assigned ten lambs to each of the five treatment diets containing 10, 12, 14, 16 and 18% CP to determine the effects of dietary CP level on performance, serum GH and IGF-1 concentrations. The initial body weight was similar for all lambs and averaged 26.20 +/- 1.10 kg. Final body weight of lambs were 37.31 +/- 1.46, 39.60 +/- 1.18, 39.85 +/- 1.24, 42.04 +/- 1.25 and 41.44 +/- 1.13 kg for groups fed 10, 12, 14, 16 and 18% CP, respectively and lambs fed 16 and 18% CP diet had significantly higher body weight than those of 10% CP (p<0.05). Mean concentrations of GH and IGF-1 in blood serum of lambs were not different significantly between groups.Öğe Interleukin-1 Beta and Tumour Necrosis Factor-Alpha Levels in Periapical Exudates(BLACKWELL PUBLISHING LTD, 2002) Ataoğlu, T.; Üngör, M.; Serpek, B.; Haliloğlu, S.; Ataoğlu, H.; Arı, H.Aim The aim of this study was to determine IL-1beta and TNT-alpha levels in periapical exudates and to evaluate their relationship with clinical and radiological findings. Methodology Periapical exudates were collected from root canals of 3 5 single-rooted teeth using absorbent paper points. IL-1beta and TNT-alpha levels were determined by enzyme-linked immunosorbent assays. The samples were divided into two groups according to the presence or absence of clinical signs including swelling and/or fistula, pain on palpation and/or percussion. and pus discharge from canals. Periapical exudate samples were divided into two groups according to size of periapical radiolucent area. Results The mean concentration of IL-1beta (72.79 ng mL(-2)) in periapical exudates was approximately 12-fold higher than TNT-alpha (6.17 ng mL(-1)), There was no significant correlation between these cytokines (P>0.05). IL-1beta levels in canals with larger radiolucent areas (long axis greater than or equal to1 cm) were significantly higher than those with small areas (P<0.05). There was a tendency toward higher levels of IL-1β in groups with clinical signs, but the differences were not significant (P>0.05). Conclusions Periapical exudate levels of both cytokines failed to reflect periapical disease state.Öğe The relationship among vitamin C, beta-carotene, vitamin A, progesterone and oestradiol 17-beta concentrations in plasma and cyst fluid of holstein cows with ovarian cyst(WILEY-BLACKWELL, 2008) Haliloglu, S.; Erdem, H.; Serpek, B.; Tekeli, T.; Bulut, Z.The aims of this study were to determine the concentrations of the progesterone, oestradiol-17-beta, vitamin A, C and beta-carotene in plasma and cyst fluid and to relate these values with cystic diameter and membrane thickness of Holstein cattle with ovarian luteal cyst. 1650 Holstein cows were examined for the presence of the ovarian cyst and luteal and follicular cystic ovaries were obtained following slaughtering in personal slaughterhouse in Konya-Turkey. 15 Luteal and 15 follicular cystic ovaries were distinguished by rectal palpation and by post mortem ultrasonographic examination. Plasma and cyst fluid, hormone and vitamin analyses were carried out by EIA method and spectrophotometric measurement respectively. Although there was no relationship between beta-carotene and vitamin A in plasma and cyst fluid of both cyst type and hormone concentrations, the vitamin C concentration of cyst fluid was found significantly higher in luteal cyst than in follicular cyst. Moreover, there is a positive correlation among values of the vitamin C concentrations of cyst fluid and cystic membrane thickness, plasma and the cyst fluid progesterone concentrations, but there is a negative correlation among the vitamin C concentrations of cystic fluid and oestradiol 17 beta levels of plasma and cyst fluid. In conclusion, vitamin C concentration of cyst fluid supported ultrasonographic and endocrinologic findings. Also, it can be postulated that vitamin C is probably effective on progesterone synthesis in the luteal tissue of cyst.Öğe The Relationship Between Ascorbic Acid, Oestradiol 17 Beta and Progesterone in Plasma and in Ovaries During the Sexual Cycle in Cattle(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Serpek, B.; Başpınar, N.; Haliloğlu, S.; Erdem, H.This study was carried out to detect the relationships between levels of vitamin C in plasma, corpus luteum, follicular fluid and those of progesterone and oestradiol 17 beta in Holstein cows getting through different periods of sexual cycle, presented at slaughterhouse. Prior to slaughter, blood samples from each cow were collected. After slaughter, ovaries of animals were taken then, the stage of sexual cycle was determined post-mortem. Diameters of corpora lutea and follicles were determined by ultrasonography. Follicular fluids were aspirated while corpora lutea were incised and weighted. The progesterone and oestradiol 17 beta concentrations were determined by EIA method. Levels of vitamin C were determined by spectrophotometer. Vitamin C concentrations in the corpus luteum were correlated with weights/diameters of corpora lutea as well as plasma progesterone levels. In addition, there was also a positive correlation between plasma vitamin C levels and diameter of the corpus luteum. Furthermore, a positive correlation was also evidenced between plasma progesterone levels and diameter/weight of corpora lutea. Oestradiol 17 beta levels in follicle fluids increase in parallel with an increase in follicle diameter. On the contrary, small follicles, irrespective of stage of oestrus cycle had, significantly higher vitamin C content than large follicles. In conclusion, it may be speculated that vitamin C might be used in the synthesis of collagen during formation of the corpus luteum, consumed in increased quantities while oestradiol 17 beta biosynthesis occurred.Öğe The Relationship Between Ascorbic Acid, Oestradiol 17? And Progesterone in Plasma and in Ovaries During the Sexual Cycle in Cattle(2001) Serpek, B.; Başpınar, N.; Haliloğlu, S.; Erdem, H.This study was carried out to detect the relationships between levels of vitamin C in plasma, corpus luteum, follicular fluid and those of progesterone and oestradiol 17? in Holstein cows getting through different periods of sexual cycle, presented at slaughterhouse. Prior to slaughter, blood samples from each cow were collected. After slaughter, ovaries of animals were taken then, the stage of sexual cycle was determined post-mortem. Diameters of corpora lutea and follicles were determined by ultrasonography. Follicular fluids were aspirated while corpora lutea were incised and weighted. The progesterone and oestradiol 17? concentrations were determined by EIA method. Levels of vitamin C were determined by spectrophotometer. Vitamin C concentrations in the corpus luteum were correlated with weights/diameters of corpora lutea as well as plasma progesterone levels. In addition, there was also a positive correlation between plasma vitamin C levels and diameter of the corpus luteum. Furthermore, a positive correlation was also evidenced between plasma progesterone levels and diameter/weight of corpora lutea. Oestradiol 17? levels in follicle fluids increase in parallel with an increase in follicle diameter. On the contrary, small follicles, irrespective of stage of oestrus cycle had, significantly higher vitamin C content than large follicles. In conclusion, it may be speculated that vitamin C might be used in the synthesis of collagen during formation of the corpus luteum, consumed in increased quantities while oestradiol 17? biosynthesis occurred.
