Yazar "Sezer, Ela Nur Simsek" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    The in vivo investigation of apoptotic effects of Nigella sativa on carbon tetrachloride-induced hepatotoxicity
    (NATL INST SCIENCE COMMUNICATION-NISCAIR, 2018) Uysal, Tuna; Ugurtan, Mevlut Han; Sezer, Ela Nur Simsek; Vatansev, Husamettin; Bozkurt, Meryem; Evliyaoglu, Nurcan
    Nigella sativa commonly known as black seed or black cumin has been used for medicinal purposes for centuries. Our purpose in this study is to determine the apoptotic effects of black cumin seed oil (N. sativa) on CCl4 induced hepatoxicity via molecular markers. For this aim, four experimental groups were created and each of them was intraperitoneally exposed to CCl4 (G2), CCl4 + black cumin oil (G3) and black cumin oil (G4) except from control (G1). DNA fragmentation, RT-PCR, and caspase-3 assay were carried out. According to our results, a remarkable morphological anointment was observed at the G2. The G3 had the lower fatty comparing to G2. DNA profiles were visualized and DNA fragmentation was only in the G3. PCR results showed that BAX gene expression fairly rose in the G3 and BCL-2 gene expression was only determined in the G2. Decreased caspase-3 in G2, and the increased of it's in G3, clearly supported the activation of the apoptotic pathway for injured cells. Consequently, our results indicated that the increases of caspase and BAX/BCL-2 rates demonstrated that there was an effective molecular mechanism based on black cumin induced apoptosis in rat liver which leads to the release of cytochrome-c from the mitochondria to the cytosol, finally activating caspase-3 and resultant apoptosis.
  • Küçük Resim Yok
    Öğe
    Karyological studies of four endemic Centaurea L. species
    (UNIV FLORENCE BOTANY INST, 2015) Uysal, Tuna; Bozkurt, Meryem; Sezer, Ela Nur Simsek; Ertugrul, Kuddisi; Tugay, Osman
    In this paper, four endemic Centaurea species were investigated in terms of their chromosome numbers and karyomorphology. The chromosomal counts confirmed the results of previous reports, that the genus Centaurea has different basic chromosome numbers. Centaurea yaltirikii and Centaurea demirizii are diploid taxa. Centaurea yaltirikii has 2n = 18 chromosomes, and therefore has a basic chromosome number of nine, which is common in the genus Centaurea. A local endemic species, Centaurea demirizii has 2n = 16 chromosomes and a different basic chromosome number of x = 8, and this basic number is relatively rare for Centaurea. Centaurea leptophylla and Centaurea saligna are tetraploid with 2n = 36 chromosomes. We found predominance of chromosomes being metacentric and submetacentric. The results increase our karyological knowledge of these species. Moreover, this paper gives short taxonomic and morphologic notes to complement the information provided by Flora of Turkey with regards to the studied species.
  • Küçük Resim Yok
    Öğe
    Karyomorphological study of five Turkish endemic Rhaponticoides Vaill. (Asteraceae, Cardueae) species
    (TAYLOR & FRANCIS LTD, 2016) Uysal, Tuna; Sezer, Ela Nur Simsek; Bozkurt, Meryem; Tugay, Osman; Ertugrul, Kuddisi; Demirelma, Hakki; Dural, Huseyin
    In this paper, we aimed to determine the chromosomal features of Turkish endemic Rhaponticoides species. The chromosome numbers and karyomorphology of five species were examined. The chromosomal counts confirmed the previous reports of 2n = 30 given for Rhaponticoides species growing in different countries. Although R. iconiensis had a karyotype formula of 28m + 2sm, the remaining four species all had 26m + 4sm. Rhaponticoides aytachii and R. hierroi were especially similar in asymmetry indices. Rhaponticoides iconiensis and R. phytiae had satellites in the fourth and second chromosome pairs, respectively. Having the most symmetrical chromosomes, R. amasiensis displayed an important relationship with R. iconiensis and R. phytiae in terms of total chromosome length (TCL). If we make an overall assessment in terms of chromosomal asymmetry, chromosomes indices continues in the low range for each species and this low asymmetry indeces indicate that the species have symmetric chromosomes as karyomorphology. In conclusion, this is the first report which describes the karyotypes and chromosomal asymmetry indices of Turkish endemic Rhaponticoides species.