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    First report on seroprevalence of bluetongue, border disease and peste des petits ruminants virus infections in sheep in kyrgyzstan
    (Agricultural Research Communication Centre, 2014) Yapici O.; Bulut O.; Avci O.; Kale M.; Tursumbetov M.; Yavru S.; Simsek A.
    In this study, the presence of antibodies against Bluetongue Virus (BTV), Border Disease Virus (BDV) and Peste des Petits Ruminants virus (PPRV), were evaluated for the first time in Kyrgyzstan. A total of 655 serum samples were collected from healthy sheep (Jaydara breed) from different regions of Kyrgyzstan (144 from Issik Gol region, 208 from Narin region, 189 from Talas region and 114 from Çuy region). Commercially available competitive ELISA kits were used to detect antibodies against tested samples. Seroprevalence was found to be 36.94%, 7.32% and 35.11% against BTV, BDV and PPRV infections, respectively. Significant differences in antibody prevalences were found among both BTV-BDV and BDV-PPRV groups (P< 0.001) only. This is the first report on seroprevalence of viral infections for BTV, BDV and PPRV in the Jaydara breed of sheep within Kyrgyzstan. © 2014, Agricultural Research Communication Centre. All rights reserved.
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    Use of aluminium hydroxide and calcium phosphate as delivery molecules for TGF-?1
    (2007) Ucan U.S.; Yildirim S.; Simsek A.; Ortatatli M.; Erganis O.
    Cytoxic effects and adsorbance properties of the adjuvants (aluminium hydroxide and calcium phosphate) were examined by means of Cytotoxicity assay, ELISA and subcutaneous implantation test. The highest concentrations of aluminium hydroxide and calcium phosphate adsorbed with TGF-?1 that produced no cytotoxicity on Verocells were found to be 0.098 mg and 2.5 mg respectively. The calcium phosphate adjuvant adsorbed less TGF- ?1 than aluminium hydroxide. By in vitro experiments, aluminium hydroxide served as more suitable carrier agent for TGF-?1.