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Öğe Antifungal activities of different essential oils against anise seeds mycopopulations(M H SCHAPER GMBH CO KG, 2016) Starovic, Mira; Ristic, Danijela; Pavlovic, Snezana; Ristic, Mihailo; Stevanovic, Milos; Al Juhaimi, Fahad Y.; Svetlana, NaydunThe aim of this study was to investigate the possibility of biological control of fungal species isolated from anise seeds using essential oils from medicinal plants: mint (Mentha spicata L.), sage (Salvia fruticosa L.), rosemary (Rosmarinus officinalis L.), anise (Pimpinella anisum L.), bitter fennel (Foeniculum vulgare spp. piperituum L.) and myrtle (Myrtus communis L.). Ten fungal species isolated from anise seeds: Bipolaris/Drechslera sorociniana, Fusarium subglutinans, F. vertricilioides, F. oxysporum, F. tricinctum, F sporotrichioides, F. equiseti, F. incarnatum, F proliferatum and Macrophomina phaseolina, were used in this experiment The minimum inhibitory concentrations (MIC) were determined by micro-dilution method using selected essential oils (EOs). A qualitative and quantitative chemical analyses of EOs were carried out. All EOs exhibited a significant antifungal activity against all tested fungal isolates. The myrtle EO proved to be the most potent one (MIC 0.0003-3.25 mg/mL, then mint 0.0003-7.75 mg/mL and sage 0.0003-10 mg/mL). All tested fungi were observed to have a susceptibility to all selected essential oils. These results suggest the possibility for application of the EOs in biological control of anise production.Öğe Chemical Composition and Antifungal Activity of Lavender (Lavandula stoechas) Oil(NATURAL PRODUCTS INC, 2018) Özcan, Mehmet Musa; Starovic, Mira; Aleksic, Goran; Figueredo, Gilles; Al Juhaimi, Fahad; Chalchat, Jean-ClaudeThe essential oil of Lavandula stoechas was examined by GC and GC-MS. Discs (5 mmi.d.) of the tested fungi (Alternaria alternata, Fusarium oxysporum and Botritys cinerea) were inoculated separately onto each assay plate and incubated at 25 degrees C for 7 days. The oil yield of dried parts (v/dw) obtained by hydro distillation was 2.9%. Thirty-two compounds representing 98.3% of the essential oil were determined. Linalool (49.9%), linalyl acetate (14.4%), lavandulyl acetate (5.7%), alpha-terpineol (5.6%), terpinene-4-ol (5.1%), lavandulol (3.7%), (E)-beta-ocimene (2.6%) and (Z)-beta-ocimene (2.4%) were identified as the main constituents of the oil. In addition, both doses of the lavender oil showed varying levels of inhibitory effects on the mycelial growth of tested fungi used in the experiment. The results demonstrated the strongest effect on B.cinerea, followed by A.alternata and F.oxysporum. The inhibitory effect is probably dependent on the concentration of essential oils.