Yazar "Sur E." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Detennination of the stages of the sexual cycle of the bitch by direct examination
    (2011) Aydin I.; Sur E.; Ozaydin T.; Dine D.A.
    The aim of this study was to determine the stages of the sexual cycle of the bitch by direct examination and also to assess the reliability of this new technique by comparing it with the classical staining techniques used in bitches. Forty mixed-breed bitches, of different ages and sexually mature were used in this study. A total of 120 vaginal smear samples were collected using a cotton swab, three from each bitch. The collected samples were air dried and coded. One of the prepared samples from each bitch was stained with May-Gnmwald Giemsa and the second sample was stained with Papanicolaou. The third sample was left unstained for direct examination. The stages of the sexual cycle were determined using all of the samples. The researcher who evaluated the samples did not have information about the coding system. The evaluation was made blindly and the results were compared after determination of the stages of the sexual cycle from all of the samples. The sexual cycle stages determined with the May-Gnmwald Giemsa and Papanicolaou techniques were completely consistent with each other. However, when the direct examination technique was compared with the classical staining techniques, there was a significant difference in the proestrus, diestrus and anestrus stages of the cycle (p<0.05) while there was no significant difference (p>0.05) in the estrus stage of the cycle. In conclusion, it was found that when determining the stages of the sexual cycle of the bitch by vaginal cytology, the direct examination technique was reliable only in detecting the estrus stage of the cycle. © Medwell Journals, 2011.
  • Küçük Resim
    Öğe
    Light microscopic investigations on the circumvallate papillae of the young and aged akkaraman sheep
    (Veteriner Fakultesi Dergisi, 2012) Unsal S.; Cuce H.; Celik I.; Sur E.; Ozparlak H.
    In this light microscopic study, morphometric parameters of the circumvallate papillae and the number of their taste buds in tongues of young (6-9 month-old) and aged (7 year-old) Akkaraman sheep were compared in order to determine general morphology and whether there were age-related differences in general morphology and numbers of the taste buds between young and aged animals. The tongues were obtained from a local slaughterhouse. Tissue samples containing dorsal lingual papillae of 32 tongues (16 young and 16 aged) from both sexes (equal numbers of the males and females) were used for histological investigation. The mean number and diameter of the circumvallate papilla, mean number and diameter of the taste buds, and the taste bud number per circumvallate papilla were determined under the light microscope. There were no significant differences between the young and aged animals in the mean number and diameter of circumvallate papillae, the taste bud number and the diameter, taste bud number per papilla. Nevertheless, the aged animals had larger circumvallate papillae. These results showed that number of the taste buds and circumvallate papilla did not change with aging in Akkaraman sheep. Because that general morphology of the circumvallate papillae and their taste bud numbers did not change even in older animals, any age-related differences in the sense of taste can not be attributed to gross degenerative changes in taste buds of circumvallate papillae.
  • Küçük Resim
    Öğe
    Selective gray and white matter staining of the horse spinal cord
    (Veteriner Fakultesi Dergisi, 2012) Bolat D.; Bahar S.; Sur E.; Selcuk M.L.; Tipirdamaz S.
    The ratio of gray and white matter is an important clinical parameter in the diagnosis of diffuse and compressive diseases of the spinal cord. Although histological methods are used to determine this parameter, there are some difficulties encountered in histological studies related to tissue size. The aim of this study was to evaluate possible modifications to overcome these difficulties. In the study, nine tissue samples taken from the C6 segment of a female Shetland pony and selected by systematic random sampling were used. The dehydration process of the spinal cord of the horse was supported by applying a vacuum. Paraffin blocks were prepared and cut into 10 ?m sections to be stained separately with the different staining methods. Six different staining methods, including Modified May - Grunwald - Giemsa (MMGG), were compared and used to image entire slides. The stains, Hematoxylin & eosin (H&E), May-Grunwald-Giemsa (MGG), Masson's trichrome (MT), AgNORs, Kluver Barrera (KB) and MMGG, were evaluated macroscopically and microscopically by participants who were unaware of which staining methods had been used. The staining methods were scored from worst (1) to best (5) using a Likert scale. Vacuum application was found to reduce the difficulties related to inadequate tissue dehydration. MMGG was selected as the best staining method in differentiating gray and white matter in the spinal cord of the horse.