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    Characterization of consolida s.f. gray (ranunculaceae) taxa in turkey by seed storage protein electrophoresis [Tohum Depo Protein Elektroforezi ile Türkiye'deki Consolida S.F. Gray (Ranunculaceae) taksonlarinin Karakterizasyonu]
    (2010) Ertugrul K.; Arslan E.; Tugay O.
    Objective: This research aimed to investigate the genetic relations of Consolida S. F. Gray species collected in Turkey using seed storage proteins. This study has become significant as the taxonomic status of genus Consolida changes often. Methods: Mature seeds of 22 Consolida species were collected. Total protein were isolated from seeds collected for each species. Sodium Dodecyl Sulphate-Poly-carylamide Gel Electrophoresis was performed by a standard method on a vertical slab gel. After electrophoresis, the protein bands were visualized by staining with Coomassie Brillant Blue G-250. The polymorphic bands were scored visually as present (1) or absent (0). Genetic similarity among taxa was estimated basing on Nei's homology. Results: In all, 74 polypeptide bands of different sizes ranging from 25 to 140 kDa were observed in the 22 taxa of the genus Consolida. Cluster analysis was performed using the unweighted pair-group method and arithmetic averages in Bio1D++ computer program. According to similarity coefficients, the genus Consolida was separated into the main two groups. It was discovered that the first group consisted of the species of Consolida with 5-lobed corolla, and in the second main group were the species with 3-lobed corolla. Conclusion: It is concluded that seed storage protein profiles could be useful markers in the studies of genetic relations of Consolida species. The grouping as a result of the dendogram has also supported the relationship in Flora of Turkey. 2010 © TurkJBiochem.com.
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    Comparison of essential oil compositions of some natural and cultivated endemic sideritis species
    (2011) Gümüşçü A.; Tugay O.; Kan Y.
    In this study, some yield components and essential compositions of some endemic Sideritis species were investigated. These species are very important for its economical value and utilization for human health. This study carried out at the experimental fields of Cumra High Educational High College and laboratories of Department of Field crops, Faculty of Agriculture, in 2008 and 2009 years. Some characteristics of natural and cultivated plants were determined and essential oil compositions were given comparatively. The collected and cultivating species were Sideritis congesta P.H. Davis and Sideritis condensata Boiss. & Heldr. Essential oil ratio of the cultivated plants were for S. congesta and S. condensata, 0.11%, 0.016%, respectively and natural plants were 0.027%, 0.020%, respectively. According to analyses, the most important components were (3-pinene (28.43%), a-pinene (20.14%), cubebol (16.98%) for cultivated S. congesta; caryophyllene (23.96%), germacrene (23.91%) for cultivated S. condensata. The other components of natural plants were (3-pinene (19.83%), a-pinene (14.61%) for natural S. congesta; caryophyllene (0.67%), germacrene (0.62%) for natural S. condensata.
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    In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss. [Capacidad antioxidante in vitro y caracterización fitoquímica de Eryngium kotschyi Boiss.]
    (Academic Association of Pharmaceutical Sciences from Antofagasta (ASOCIFA), 2020) Paşayeva L.; Şafak E.K.; Arıgün T.; Fatullayev H.; Tugay O.
    Context: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH., ABTS+. and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 ± 1.088 mg gallic acid equivalent/gextract) and total flavonoid content (86.978 ± 0.650 mg catechin equivalent/gextract) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH. (IC50 = 0.264 ± 0.040 mg/mL), ABTS+. (at a concentration of 0.125 mg/mL equivalent to 0.497 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 1476 ± 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 ± 0.703 µg/mgextract), chlorogenic acid (80.405 ± 0.170 µg/mgextract), isorhamnetin 3-O-rutinoside (72.280 ± 0.33 µg/mgextract), rutin (63.020 ± 0.052 µg/mgextract) and caffeic acid (55.153 ± 0.523 µg/mgextract). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents. © 2020 Journal of Pharmacy & Pharmacognosy Research