Yazar "Ucan, Uckun Sait" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antibacterial and Antibiofilm Effects of Boron on Different Bacteria(HUMANA PRESS INC, 2016) Sayin, Zafer; Ucan, Uckun Sait; Sakmanoglu, AsliBoron (B) compounds are used in many fields ranging from medicine to industry. In this study, boric acid (BA) and disodium octaborate tetrahydrate (DOT) were evaluated for their antibacterial effects and antibiofilm capacities on selected strains of clinical and type cultures that are of veterinary concern (Staphylococcus aureus ATCC 25923, Aeromonas hydrophila ATCC 19570, Pseudomonas aeruginosa ATCC 27853, Brucella melitensis Rev1 and field isolates of Vibrio anguillarum, Aeromonas hydrophila, Yersinia ruckeri, Pseudomonas aeruginosa, Lactococcus garvieae, and Brucella abortus). Also, the inhibition of biofilm was monitored by scanning electron microscopy. The lowest MIC values of BA and DOT were measured, by broth method using microdilution, from Pseudomonas aeruginosa ATCC 27853, and were 0.385 and 0.644 mg/ml, respectively. Staphylococcus aureus was the most resistant to both BA and DOT. Using the microplate method, we observed that the strongest positivities for biofilm production were presented by Pseudomonas aeruginosa ATCC 27853 and also a clinical isolate of Lactococcus garviea. Lower values in the MIC scores for both B compounds were tested by measuring the inhibitory effect on biofilm production. We found that all the bacterial strains inhibited biofilm formation with the exception of the Pseudomonas aeruginosa strains for BA only and an isolate of Lactococcus garviea for DOT only. Such effects by BA and DOT are worth discussing in order to find novel approaches for different functions in medicine and industry using the bacteria tested.Öğe Canine brucellosis in Turkey from an environmental perspective(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Ucan, Uckun SaitBrucellosis is a zoonotic disease that affects not only livestock with economical losses but also human beings by causing serious health problems worldwide. Canine brucellosis has largely been overlooked in the world. In Turkey, brucellosis has also been one of the major concerns for decades. Canine brucellosis in this country has been reviewed and discussed from an environmental perspective in this review. As a part of prevention measures, one of the urgent practices that should be applied is to expand the routine examinations to include serological testing for all animals on arrival to kennels and then to decide the fate of the seropositive animals. It is thought that by giving wider information about stray dogs and wild Canidae in a common but not quite large habitat like that in Turkey, new studies could provide more understanding of canine brucellosis in one common environment.Öğe Comparison of five methods for isolation of DNA from Mycoplasma cynos(ELSEVIER SCIENCE BV, 2017) Sakmanoglu, Ash; Sayin, Zafer; Ucan, Uckun Sait; Pinarkara, Yasemin; Uslu, Ali; Erganis, OsmanExtraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute (TM) gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute (TM) gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods. The results were evaluated based on the purity and amount of DNA obtained from one Mycoplasma colony. Eight strains of Mycoplasma cynos isolated from the broncho-alveolar lavage fluid of dogs were used. The GenElute (TM) gel extraction protocol was the most efficient among all the methods tested in this study as it yielded the highest amount and the purest quality of DNA (199.3 +/- 0.744 ng/mu 1) from a single colony. Among the methods tested, the GenElute (TM) gel extraction method is the most rapid, sensitive, and simple method for DNA extraction from Mycoplasma. This procedure may also prove useful for extracting DNA from other Mycoplasma species.Öğe Concentrations of total serum immunoglobulin e, a, g and m in stray dogs with healthy and dermatological problems(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2013) Maden, Mehmet; Birdane, Fatih Mehmet; Ucan, Uckun Sait; Altunok, VahdettinIn this study, clinical and laboratory findings along with serum IgE, IgA, IgM and IgG concentrations were determined in stray dogs (healthy and with dermatological problems). It was determined that skin lesions were localized in the rate of 69.8% (n=44) and generalized in the rate of 30.2% (n=19) of sick animals. Serum IgE concentration in stray dogs with dermatologic problems was significantly higher (P<0.001) than that of healthy dogs whereas serum IgM and G concentrations were significantly lower (P<0.05). It was evaluated that, serum total immunoglobulin concentrations could be useful in making contribution to differential diagnosis of skin diseases in stray dogs.Öğe Effect of parenteral administration of vitamin b to goats on performance, lice (phthiraptera) infestations and cellular immunity(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2017) Uslu, Ugur; Balevi, Tahir; Ucan, Uckun Sait; Ceylan, Onur; Uslu, AliThis study was carried out on 20 of 8-10 months old Anatolian black goats which were healthy or Linognathus africanus infested (n=10 each). Each goat was housed individually all along the study. Five of ten louse-infested goats were administered vitamin B complex (Benefor enj) intramuscularly at a dose of 40mL/goat. Remaining goats (n=5) were not given vitamin B complex. The other goats that have not been infested with L. africanus were grouped exactly the same as above. All the animals (n=20) were first sensitized and then measured for hypersensitivity reaction in terms of cellular immune response. Infested goats given vitamin B complex were shown a decrease in L. africanus infestation. The other 5 number of infested goats that not given vitamin B complex were detected with an increase in the louse infestation. At the end of the trial, the higher live weight was recorded in healthy goats than those not administered vitamin B complex.Öğe Genotypic Characterization of Bordetella bronchiseptica Strains Isolated from Stray and Pet Dogs(UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2016) Sayin, Zafer; Sakmanoglu, Asli; Erganis, Osman; Ucan, Uckun Sait; Hadimli, Hasan Huseyin; Aras, Zeki; Sanioglu, GokcenurBordetella bronchiseptica (B. bronchiseptica) is the most important pathogen associated with kennel cough in dogs. The presence of B. bronchiseptica in pet dogs and shelter dogs with clinical respiratory disease was investigated in present study. The genetic relatedness among the strains was determined to evaluate the role of stray dogs in spread of B. bronchiseptica to pet dogs by detection of virulence genes such as filamentous hemagglutinin (fha), pertactin (prn) and dermonecrotic toxin (dnt). We also performed the random amplified polymorphic DNA (RAPD) assay. A total of 96 B. bronchiseptica were isolated from stray and pet dogs. The fha, prn and dnt virulence genes were detected in 86, 83.3 and 61.4% strains, respectively by polymerase chain reaction (PCR) techniques. The most common genotype from stray and pet dogs was fha+prn+dnt+ as detected in 37.5% and 11.4% of all the strains, respectively. The RAPD assay showed that 3 different patterns were obtained from 96 B. bronchiseptica strains. Sixty one (63.5%) of them were clustered in one main group and then further placed in another 2 sub-groups by RAPD assay. Genetic association was seen between the B. bronchiseptica strains from stray and pet dogs. In conclusion, this study revealed that B. bronchiseptica is present at a higher rate in stray dogs than pet dogs. Stray dogs might have a significant role in the transmission of B. bronchiseptica to pet dogs. (C)2016 PVJ. All rights reservedÖğe Investigation of Brucella canis Seroprevalence in Brucellosis Suspected Cases(ANKARA MICROBIOLOGY SOC, 2013) Yuksekkaya, Serife; Aras, Zeki; Ucan, Uckun SaitBrucella canis which is the main etiologic agent of brucellosis in dogs, can be transmitted to man. It causes mild or asymptomatic infection in human compared with other Brucella species. B.canis can be transmitted to man either by laboratory accidents or contact with infected dogs. Since B.canis infections in humans are not routinely investigated in hospitals in Turkey, the data are limited to reveal the current status of B.canis infections in people in our country. The purpose of this study was to determine the seroprevalence of B.canis infection in brucellosis-suspected cases. The study was conducted at Konya Education and Research Hospital, (located at Central Anatolia of Turkey) during March-August 2010 period. Serum samples were obtained from 1000 patients (age range: 15-65 years; 652 of them were women) presented with brucellosis-like symptoms, including fever, headache, night sweats, appetite loss, weakness, arthralgia and myalgia. Rose Bengal Plate Tests (Seromed, Turkey) for smooth Brucella species were negative in all serum samples. Rough type B.canis antigen was prepared with B.canis NCTC 10854 strain for serodiagnosis. Antibody responses to B.canis in the serum samples were investigated by rapid slide agglutination test (SAT) and modified plate agglutination test (MPAT). Of the 1000 sera tested, 34 (0.34%) were found to be positive with SAT while the remaining were found negative. MPAT was used for the detection of antibody titer and 22 (0.22%) out of 1000 sera were found positive with MPAT (one had 1/48, five had 1/96, six had 1/192, six had 1/384, four had 1/768 titers). Among 22 positive patients, 17 were female and five were male, and the difference between the genders was found statistically significant (p< 0.05). It was concluded the use of both S and R antigens in the serological tests applied for the diagnosis of brucellosis in our country will supplement both diagnosis and seroepidemiological data related to brucellosis.Öğe Mycoplasma infections in dairy cattle farms in Turkey(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2016) Sayin, Zafer; Sakmanoglu, Asli; Ucan, Uckun Sait; Uslu, Ali; Hadimli, Hasan Huseyin; Aras, Zeki; Ozdemir, OzgurMycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) isolates were identified as Mycoplasma bovis (M. bovis). Eleven (6.3%) isolates were identified as M. alkalescens, 2 (1.1%) were M. canis, and 10 (5.8%) were M. bovigenitalium. The isolation rate was found to be increasing annually. In conclusion, mycoplasmosis is a common problem in Holstein dairy cattle farms in Turkey, and M. bovis is the most frequently encountered cause of mycoplasma infections. The isolation rate seems to have increased in correlation with increased live cattle imports. Additionally, M. alkalescens and M. canis were isolated and identified in respiratory tract infections in cattle from Turkey for the first time.Öğe Serodiagnosis of brucella canis infection in dogs by a dipstick enzyme immunoassay(2010) Ucan, Uckun Sait; Aras, Zeki; Semacan, AhmetUçan US, Aras Z, Semacan A. Köpeklerde Brucella canis enfeksiyonunun dipstik enzim immunoassay ile teşhisi. Eurasian J Vet Sci, 2010, 26, 2, 109-112 Amaç: Köpeklerin brusellozunun teşhisi bakteriyolojik, serolojik ve moleküler yöntemlerle yapılmasına rağmen pratisyen veteriner hekimlerin hızlı ve güvenilir bir teste ihtiyaçları vardır. Bu çalışmada hızlı serolojik tanı için Dipstik EIA geliştirilmesi amaçlandı. Gereç ve Yöntem: Test, Çabuk Lam Aglütinasyon ve indirekt ELISA testleri ile karşılaştırıldı. Bulgular: Testin, iELISA’ya göre %82 sensitivite ve %95 spesifiteye sahip olduğu belirlendi. Öneri: Testin yüksek spesifitesinden dolayı saha şartlarında tarama testi olarak kullanımı önerilir.
