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Öğe Cytotoxicity evaluation of dentin contacting materials with dentin barrier test device using erbium-doped yttrium, aluminum, and garnet laser-treated dentin(SAGE PUBLICATIONS LTD, 2014) Ulker, H. E.; Ulker, M.; Botsali, M. S.; Dundar, A.; Acar, H.Objectives: The effect of dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device using erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser-treated dentin. Methods: The test materials (iBond (R), G-Bond (TM), and Vitrebond (TM)) were applied on laser-treated or untreated dentin discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated by enzyme activity and related to a nontoxic control material. The mean values of control tissues were set to represent 100% viability. Data were analyzed using Kruskal-Wallis and Mann-Whitney U test. Results: Vitrebond was the most toxic material for both laser-treated and untreated dentin. On untreated dentin, G-bond was cytotoxic to the pulp-derived cells (p < 0.05), and iBond was similar to the negative control group (p > 0.05). However, G-Bond and iBond were not cytotoxic when they were applied to Er: YAG laser-treated dentin (p > 0.05). Conclusion: Er: YAG laser treatment of dentin may protect the pulp cells from toxic substances of dentin contacting restorative materials; however, this effect is material related. Taking into consideration the limitations of this in vitro study, the Er: YAG laser treatment of dentin before restoration might be an option for decreasing the cytotoxic effects of the dental materials. Further research is required for clinical applications.Öğe Efficacy of Using Erbium, Chromium-Doped: Yttrium Scandium Gallium Garnet Laser-Treated Dentine in a Dentine Barrier Test Device(WOLTERS KLUWER MEDKNOW PUBLICATIONS, 2018) Ulker, M.; Ulker, H. E.; Botsali, M. S.; Dundar, A.Objectives: The aim of this study was to evaluate the efficacy of using erbium, chromium-doped: yttrium scandium gallium garnet (Er,Cr:YSGG) laser-treated dentine in a dentine barrier test device. Materials and Methods: The test materials (G-Bond (TM) and Vitrebond (TM)) were applied onto laser-treated or laser-untreated dentine discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated based on enzyme activity and compared to a nontoxic control material. The mean of the control was set to 100% viability. Data were analyzed using the one-way analysis of variance and the Tukey's honest significant difference tests. Results: The responses of bovine pulp-derived cells after exposure to G-Bond and Vitrebond on Er, Cr: YSGG laser-treated and laser-untreated dentin were statistically different from negative control group (P < 0.05). Conclusion: Er,Cr:YSGG laser treatment was not successful enough in decreasing the cytotoxic effects of the dental materials. Different parameters of Er,Cr:YSGG laser or different laser types could be investigated as an alternative to minimizing the cytotoxic effects of dental materials.Öğe Influence of Operator Experience on In Vitro Bond Strength of Dentin Adhesives(Quintessence Publishing Co Inc, 2012) Unlu, N.; Gunal, S.; Ulker, M.; Ozer, F.; Blatz, M. B.Purpose: To examine the influence of operator experience level on shear bond strength (SBS) of a self-etching adhesive (Clearfil SE Bond [CSE], Kuraray) and an etch-and-rinse adhesive (Single Bond [SB], 3M ESPE). Materials and Methods: Flat dentin surfaces were created on 120 extracted human third molars. Bonding agents and composite resin were applied and light cured according to manufacturers' directions (n = 15). Four operators with different levels of experience in operative dentistry performed the same specimen preparation steps independently: group 1: specialist in operative dentistry; group 2: post-graduate student; group 3: undergraduate dental student; group 4: private general practitioner. Specimens were stored in distilled water for 24 h at 37 degrees C before SBS testing. Statistical analyses were carried out with Mann-Whitney U- and Kruskall-Wallis tests. Results: For CSE, mean SBS and standard deviations were: (1) 54.4 +/- 15.6(a), (2) 55.3 +/- 7.6(a), (3) 49.4 +/- 10.5(ab), (4) 41.6 +/- 13.1(b). For SB, they were: (1) 28.6 +/- 10.3(c), (2) 32.7 +/- 11.2(c), (3) 17.5 +/- 5.6(d), (4) 24.4 +/- 9.5(cd). Groups with the same superscript letters are not significantly different. CSE showed higher bond strengths than SB in all groups (p < 0.05). There was no significant difference in mean bond strengths between groups 1 and 2 for each adhesive (p > 0.05). Depending on the adhesives, groups 3 and 4 were significantly different from groups 1 and 2, but not different from each other. Conclusion: Operator experience influences dentin bond strength values of dentin adhesives. An increased level of experience with a specific adhesive system and the associated application procedures correlates with higher SBSs.