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    Effects of different salt concentrations and Rhizobium inoculation (native and Rhizobium tropici CIAT899) on growth of dry bean (Phaseolus vulgaris L.)
    (ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2011) Uyanoz, Refik; Karaca, Ummuhan
    Research was conducted to determine the effect of salinity on Rhizobium and growth of dry bean. A commercial cultivar (Akman 98) of dry bean (Phaseolus vulgaris L.) was inoculated with Rhizobium tropici strain CIAT899 and native Rhizobium in solution culture with different salt concentrations (control, 5, 10, 20 and 40 mmol(-1)) added before inoculation. The results indicated that population of R. tropici strain CIAT899 and natural Rhizobium were decreased with salinity levels. However, the population count train CIAT899 was inversely proportional to salt concentration with high growth (8.0-9.0 x 10(5) cfu ml(-1)) at lower concentrations of control 5 mmol(-1) and low growth (4.0-6.0 x 10(5) cfu ml(-1)) at higher salt concentrations 01 20 mmol(-1). The plant root and shoot dry weight, chlorophyll content, plant height, root length, total nitrogen, symbiotic efficient and efficient rate were affected by salt stress in tested plant and both inoculations. Total nitrogen content decreased significantly at the highest level of salinity. It is important to decrease the soil salinity and to improve the yield of dry bean (P. vulgaris L) as well as the Rhizobium sp. associated with it. (C) 2011 Elsevier Masson SAS. All rights reserved.
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    Effects of microbial inoculation, organic and chemical fertilizer on yield and physicochemical and cookability properties of bean (Phaseolus vulgaris L.) seeds
    (UNIV PHILIPPINES LOS BANOS, 2007) Uyanoz, Refik; Akbulut, Mehmet; Cetin, Ummuhan; Gultepe, Necla
    A field experiment was carried out to study the effects of Rhizobium, Arbuscular mycorrhiza (AM) inoculation, cattle manure and chemical fertilizer on yield, 100-seed weight, total defects and nutrient accumulation of bean (Phaseolus vulgaris L.) seeds. Inoculation and organic fertilizer treatments significantly (p <= 0.05) increased yield, 100-seed weight, non-soakers and hydration coefficient in the absence of biological inoculation, cattle manure and chemical fertilizers. On one hand, AM and Rhizobium inoculum showed the highest correlation (r = 0.97) between the non-soaker percent and the total defect percent. On the other hand, the highest protein content was determined for Mycorrhiza and control treatments. Based on the results, Rhizobium and AM inoculation is a promising fertilizer because it is inexpensive, easy to handle and improves plant growth. Generally, microbial inoculation, organic and inorganic fertilizers not only increased plant growth and yield but also improved seed quality and nutritional value. The types of treatments used considerably enhanced the macro and micronutrients in the cultivar seed compared with the control.
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    EFFECTS OF MYCORRHIZAL FUNGI ON TOLERANCE CAPABILITY OF CORN GROWN UNDER SALT STRESS CONDITION
    (TAYLOR & FRANCIS INC, 2014) Boyacioglu, Taliha Uysal; Uyanoz, Refik
    A greenhouse experiment was conducted to determine the effect of salinity on the efficacy of two arbuscular mycorrhizal fungi (AMF), Glomus mossea and natural mycorrhiza, of Glomus species, was investigated in terms of growth and nutrition of corn plant (Zea mays L). Plants were grown under different salinity levels imposed by 2.0, 2.5, 3.5, 5.0, 8.0, 12.0 dS m(-1)of Hoagland's Solution [sodium chloride (NaCl), sodium sulfate (Na2SO4)(,) Calcium dichloride (CaCl2), and magnesium sulfate (MgSO4) 7:9:3:1 ratio, respectively]. Both types of mycorrhizal fungi did not display significant protection in the host plant against the detrimental effects of the soil salinity. The effect of inoculation on growth varied only with the level of salinity. Maximum root colonization and spore numbers were observed in plants cultivated with low salinity levels. It was found that significant interaction between AMF x Salinity level for calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), and manganese (Mn) of shoot, and for Zn and Mn, of root.
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    GROWTH, MINERAL ACQUISITION AND ION UPTAKE RESPONSE OF COMMON BEAN (PHASEOLUS VULGARIS L.) TO INOCULATION WITH RHIZOBIUM UNDER DIFFERENT SALT
    (PARLAR SCIENTIFIC PUBLICATIONS (P S P), 2016) Karaca, Ummuhan; Uyanoz, Refik
    This study was conducted in order to evaluate the symbiotic effectiveness of Rhizobium Leguminosarum by. phaseoli strains isolated from common bean in comparison to standard bacterial culture under NaCl salinity stress conditions. The effect of seed Rhizobium inoculation and NaCl levels (control 0, 5 mM, 10 mM, 20 mM and 40 mM) on mineral accumulation of common bean (Phaseolus vulgaris L.) was studied under greenhouse condition. A commercial common bean cultivar (Akman 98) was inoculated with R. tropici strain CIAT899 and local Rhizobium. The results showed that the application of salt significantly decreased both concentration and the accumulation of most micro and macronutrients both in leaf and biomass. The bacterial inoculation had a significant effect on Ca++, Fe accumulation and ion uptake (K+ + P + Ca++) + Na+ and on P, Mg-++,Mg- Mn and Zn contents of leaf. However, the effect of inoculation on Na+, K+, Na+/K+, N and Cu contents of the leaf were insignificant. Significant interaction was detected between inoculation and salt concentration. Inoculation with Rhizobium phaseoli could work as a useful means for alleviating salinity stress in salt-sensitive plants.
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    MORPHOLOGICAL IDENTIFICATION OF VESICULAR-ARBUSCULAR MYCORRHIZA ON BULBOUS PLANTS (TAURUS MOUNTAIN IN TURKEY)
    (INST BIOLOSKA ISTRAZIVANJA SINISA STANKOVIC, 2015) Karaarslan, Emel; Uyanoz, Refik; Dogu, Suleyman
    This study was conducted to investigate the morphological identification of vesicular-arbuscular mycorrhiza (VAM) on bulbous plants in the Taurus Mountains in Turkey. Thirteen soil samples and bulbous roots were taken from the rhizosphere of bulbous plants. The soils were analyzed for the number of VAM spores and chemical and physical properties. In addition, the roots were examined for infection levels, and morphological identification of VAM spores was made. All tested plants are considered mycorrhizal plants. We determined three spore species (Glomus mosseae, Glomus hoi and Scutellospora calospora) from the surveyed soils. The spore distribution rates were as follows: G. Mossea 61.54 %, G. Hoi 23.07 % and Scutellospora calospora 15.38 %. Results suggest that VAM fungal spores and root colonization display variation in rhizosphere under bulbous plants in different ecological conditions.
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    Performance of partial root-zone drip irrigation for sugar beet production in a semi-arid area
    (ELSEVIER SCIENCE BV, 2016) Topak, Ramazan; Acar, Bilal; Uyanoz, Refik; Ceyhan, Ercan
    Partial root-zone drying (PRD), a water-saving irrigation strategy, is being tested in many field crops species. In this study, the effects of PRD on root yield, sugar yield, water use efficiency (WUE), and fertilizer-nitrogen use efficiency (FNUE) of field-grown sugar beet were compared with those of conventional deficit irrigation (CDI) and full irrigation (FI). The experiments were conducted at Konya-Cumra, a Central Anatolian region of Turkey, in 2012 and 2013. Five irrigation techniques were designed and three nitrogen levels were chosen for the current study. These five irrigation techniques were applied to the study crops with the help of a drip irrigation system. In FI (control), the irrigation water was applied to both sides of the root system such that 35-40% of the available soil moisture was consumed in the 0.90-m root zone. In CDI50 and CDI75, 50% and 75% irrigation water of FI, respectively, was supplied to both sides of the root system. In alternative PRD50 (APRD(50)), the half of the root system was exposed to soil drying and the other half was kept well-watered with 50% irrigation water of FI. In fixed PRD50 (FPRD50), 50% irrigation water of FI was supplied only to half of the fixed side of the root system. Furthermore, the three chosen nitrogen levels included N-100, where the plant's nitrogen requirement is met completely; N-75, where 25% reduction was made in the plant's nitrogen requirement; and N-50, where 50% reduction was made in the plant's nitrogen requirement. Although the effect of nitrogen levels on sugar beet root and sugar yields was not significant, irrigation treatments had a significant effect (5% of the level). Compared to the FI treatment, the CDI75, CDI50, APRD(50), and FPRD50 treatments decreased the standardized root and sugar yields by 6.36%, 26.97%, 19.12%, and 23.50%, respectively. APRD(50) and FPRD50 increased the standardized yield by 10.74% and 4.75% compared to CDI50, respectively. In addition, when same amount of irrigation water was used, PRD (APRD(50) and FPRD50) treatments outperformed CDI50 in WUE and FNUE of sugar beets. Compared to FI and CDI50, APRD(50) increased WUEroot by 19.8% and 8.5% and FNUEroot by 26.2% and 68.2%, respectively. The varying nitrogen levels had a significant effect on FNUEroot and FNUEsugar. The highest FNUEroot and FNUEsugar values were obtained with N-50. Further, combinations of different irrigation treatments and nitrogen levels had a significant effect on FNUEroot. (C) 2016 Elsevier B.V. All rights reserved.