Yazar "Vural H.C." seçeneğine göre listele

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    Comparison and development of a rapid extraction methods of DNA from ancient human skeletal remains of Turkey
    (2010) Vural H.C.; Tirpan A.A.
    The use of genetic technology in forensic science is applied primarily to distinguish between individials who may be the source of biological material associated with archeological remains. DNA sequences from ancient fossils have great potential for studies of phylogeny, biogeography and molecular evolution. DNA from fossils also facilitates the rigorous testing and calibration of mutation rates among related taxa, sex test and molecular divergence time. In this study, a rapid and quantitative aDNA extaction methods from human skeletal remains was developed for application of forensic science and archeometry. For that reason, DNA was extracted from ancient human bones from Mugla in Turkey. Extraction of DNA was carried out using the laboratuary handling and cleaning protocol. After cleaning of bone, small piece of ancient bones were ground to powder with a mixer mill. Aliquots of the powder were subjected to a calfication method and extracted with 0.5 M EDTA (pH 8.3) for 48 hours at 56 °C. After addition of proteinase K, solution of bone was incubated at 37 °C. Genomic DNA from supernatant was extracted automatically by using EZ1 Automatic Nucleic Acid Isolation System (Qiagen, Germany) with investigator kit (Qiagen, Ilden, Germany) and different DNA extraction methods which are modified by researcher from ancient bones. EZ1 Nucleic acid isolation method; This tehnique is quite useful for high yield and quality of aDNA isolation from human skeletal remains. In this methods, no further purification was needed for molecular analysis. Amount and purity of extracted DNA from ancient bones were measured by Spectrophotometer. In addition to spectrophotometric measurement, extracted DNA was applied to 1 % agarose gel, stained and imaged under ultraviolet (UV) irradiation. As a result, 50 ng pure DNA was extracted from ancient bones, approximately 1.8. This protocol proved to be advantageous because of its simplicity, quickness and affordable reagents, besides the high molecular weight DNA and purity achieved in a variety of fosil bone tissues from the total set obtained from Mugla in Turkey. © Internet Scientific Publications, LLC.
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    Identification of genotype and allelic frequencies of vitamin D receptor gene (Taq1) polymorphisms in T1DM patients from Turkey
    (2011) Vural H.C.; Isler O.
    AsbtractChanges in the Deoksiribonucleic acid (DNA), or polymorphisms, of the Vitamin D Receptor (VDR) cause the protein to bind more or less tightly to 1.25 Hidroksil (OH). The tighter that vitamin D binds, the stronger and longer lasting the metabolic changes are. Some of the different polymorphisms of the VDR have been associated with an increased risk for Diabetes Mellitus. This study suggests that while Taq1 polymorphisms may be functionally different it may also play a role in serum levels. Therefore, in this study, we selected TT allele of VDR gene has been associated with higher Diabetes Mellitus risk for study and investigated young adults or 100 patients with T1DM (50 women, 50 men) and 120 healthy subjects. The Polymerase chain reaction (PCR) was used for amplification of a 200 bp fragment of the VDR gene. One study found that TT genotype are over-represented in T1DM patients and those with the TT allele had a 3 fold increase in T1DM risk. In addition, the aim of the present study was to adapt PCR amplification, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the most effective DNA isolation method. The results indicate that the Taq 1 polymorphism in the VDR gene plays a significant role in protection against T1DM. Consequently, We found an association between VDR polymorphism (Taq1) and T1DM in Turk population.
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    A real-time quantitative PCR assay for quantification of c-MYC DNA in patients who suffers from leukemia
    (Lifescience Global, 2013) Vural H.C.; Ünsal S.; Tosunal G.
    The MYC cancer gene contains instructions for the production of the c-Myc protein. The c-Myc protein is known as a transcription factor or a regulator of other genes. It is a protein that binds DNA at specific sites and instructs genes whether or not they should be transcribed into messages for cells to make additional or other new proteins. Quantitative real-time PCR (qRT-PCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology, archaeometry and diagnostics and has become the method of choice for the quantification of cDNA and nDNA. Therefore, we used Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or cDNA, namely used for research both DNA. We optimized a method for monitoring quantitative real-time PCR (qRT-PCR) of c-Myc cancer gene in patients with leukemia. We describe qRT-PCR a series of protocols that illustrate the essential technical steps required to generate quantitative data that are reliable and reproducible. In addition, our aim is to also quantify extracted DNA and determine its purity and the validation of extracted DNA from patients with leukemia including active Myc gene family. We also believe these protocols will be accessible to the researchers to provide them reliable data in this protocol. These analytical methods are essential for accurate gene quantification. With reference to, advantages of qRT-PCR are a large dynamic range of quantification, no requirement for post-PCR sample handling and the need for very small amounts of starting material. The specificity, reproducibility and detection limit of the assay was examined. The assay was used to monitor c-myc DNA levels in patients with leukemia. © 2013 Lifescience Global.
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    Reproduction by seed and tissue culture of soybean (Glycine max (L.) Merr.) growing in Turkey
    (2010) Işler O.; Vural H.C.
    Human health is not achievable unless adequate amounts of nutritious and safe foods are available and accessible during all life stages. An estimated one-third of the world's population, largely in the developing world, is currently food and nutrition insecure. The most obvious use of plant products in traditional biotechnology was for food. New and even better varieties were produced when early humans learned to cross-pollinate plants and became plant breeders. Soybean breeding has resulted in significant improvements in yield potential, stability of yield, adaptation of the species to mechanical harvest, and yield protection through improved disease resistance. Due to the nature of plant science agriculture, broadly defined as a manipulation of available plant resources to meet the needs of the growing human population, the environment in which plants are grown for agricultural production continuously offers new obstacles to agricultural production. The present invention provides a tissue culture of regenerable cells from a plant, or parts thereof, produced by growing seed and a soybean plant regenerated from the tissue culture. The present invention also provides a method for developing a soybean plant in a soybean breeding program using plant breeding techniques.