Yazar "Özdil, Fulya" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes
    (WILEY, 2011) Aytekin, İbrahim; Özdil, Fulya; Zülkadir, Uğur; Boztepe, Saim; Sarıyel, Vahdettin
    BACKGROUND: The utilisation of molecular markers has increased in molecular research recently. Inter-simple sequence repeat (ISSR) markers allow the analysis of genomes without preliminary sequence information, since random primers are used. The purpose of the present study was to evaluate ISSR markers for assessing the genetic diversity of indigenous Anatolian Water Buffaloes reared in Afyon, Konya and Sivas provinces of Turkey, with a view to conservation of the gene resources. RESULTS: The 11 ISSR primers chosen for the analysis revealed a total of 110 bands, of which 76(69.09%) were polymorphic. Also, genetic similarity, polymorphic information content (PIC), resolving power (R-p) and mean resolving power ((R-p) over bar), heterozygosity (H) and Shannon index (I) were calculated as 0.9479-0.9562, 0.35 +/- 0.20, 2.73, 0.27, 0.18 +/- 0.07 and 0.28 +/- 0.11, respectively. CONCLUSION: The ISSR markers were found to be promising for assessing the genetic diversity in buffalo populations. Potential genetic parameters such as PIC, R-p, (R-p) over bar, H and I were effectively used in this study. (C) 2011 Society of Chemical Industry
  • Küçük Resim
    Öğe
    Genetic Divergence of Turkish Apis Mellifera Subspecies Based on Sequencing of ND5 Mitochondrial Segment
    (CALIFORNIA STATE UNIV, 2012) Özdil, Fulya; İlhan, Fatma
    Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. In this study, DNA sequence diversity in the ND5 region of the mitochondrial genome was investigated in 93 samples of Apis mellifera from 15 different populations in Turkey. Five novel haplotypes were revealed for the ND5 gene segment of Turkish honeybees. The number of variable sites found was 6 for this region while 2 were parsimony informative sites. The average pairwise genetic distances were 0.3% for ND5 gene. In this study, the NJ tree of ND5 gene segment were constructed with the published sequences of Apis mellifera haplotypes. This study expands the knowledge about the mitochondrial ND5 region in Apis mellifera and it is also the first comprehensive sequencing analysis of ND5 region in Turkish honeybees.
  • Küçük Resim
    Öğe
    Genetic Variation in Turkish Honeybees Apis Mellifera Anatoliaca, A. m. Caucasica, A. m. Meda (Hymenoptera: Apidae) Inferred from RFLP Analysis of Three mtDNA Regions (16S rDNA-COİ-ND5)
    (CZECH ACAD SCI, INST ENTOMOLOGY, 2012) Özdil, Fulya; Aytekin, İbrahim; İlhan, Fatma; Boztepe, Saim
    In this study, the genetic structure of Turkish honey bee (Apis mellifera L.) populations, mainly obtained from the Central Anatolian region, were investigated at three different mitochondrial regions. A total of 165 worker bees were collected from 15 different populations in ten different locations. Portions of the mitochondrial 16S ribosomal RNA (16S rDNA), cytochrome C oxidase I (COI) and NADH dehydrogenase 5 (ND5) genes were amplified by PCR and then subjected to RFLP pattern analysis using 18 restriction enzymes (these having at least one recognition site in each region were used). Nucleotide polymorphisms were revealed using restriction enzymes Bsp1431, DraI and SspI in 16S rDNA and TaqI in the COI gene segment. The polymorphisms were subsequently confirmed by direct DNA sequencing with sequences thereafter deposited in Genbank. In this study, six novel composite genotypes (haplotypes) were found in Turkish honey bee populations. The most common haplotype, type 1, was found in 12 of the sampled populations and overall accounted for 85.5% of the samples. TCS spanning network of haplotypes revealed that type 1 was the basal haplotype. Genetic distance (D) values were found to be low (0.0-0.0112) within Turkish honey bee populations. The average haplotype diversity (h) within populations was 0.082. Molecular phylogenetic analysis revealed that Konya/Sizma, Antalya/Elmali and Konya/Selcuklu populations were the most distant from all the other Turkish honey bee populations surveyed.