Evaluation of ISSR markers for genetic diversity analysis in Anatolian Water Buffaloes
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
WILEY
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BACKGROUND: The utilisation of molecular markers has increased in molecular research recently. Inter-simple sequence repeat (ISSR) markers allow the analysis of genomes without preliminary sequence information, since random primers are used. The purpose of the present study was to evaluate ISSR markers for assessing the genetic diversity of indigenous Anatolian Water Buffaloes reared in Afyon, Konya and Sivas provinces of Turkey, with a view to conservation of the gene resources. RESULTS: The 11 ISSR primers chosen for the analysis revealed a total of 110 bands, of which 76(69.09%) were polymorphic. Also, genetic similarity, polymorphic information content (PIC), resolving power (R-p) and mean resolving power ((R-p) over bar), heterozygosity (H) and Shannon index (I) were calculated as 0.9479-0.9562, 0.35 +/- 0.20, 2.73, 0.27, 0.18 +/- 0.07 and 0.28 +/- 0.11, respectively. CONCLUSION: The ISSR markers were found to be promising for assessing the genetic diversity in buffalo populations. Potential genetic parameters such as PIC, R-p, (R-p) over bar, H and I were effectively used in this study. (C) 2011 Society of Chemical Industry
Açıklama
Anahtar Kelimeler
ISSR markers, Anatolian Water Buffaloes, genetic diversity, genetic parameters, resolving power
Kaynak
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
91
Sayı
11
