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Öğe Comparison of culture, Real- time-PCR, ELISA, and histopathological examination methods for identification of Helicobacter pylori(2018) Maçin, Salih; Alp, Alpaslan; Şener, Burçin; Sökmensüer, Cenk; Orhan, Diclehan; Özen, Hasan; Kav, TaylanIntroduction: There are several methods used for the diagnosis of Helicobacterpylori infections, and there is an increasing demand for theuse of non-invasive, more rapid tests. The aim of the present study wasto compare different diagnostic methods.Methods: A total of 87 patients who had undergone esophagogastroduodenoscopywere included in the study. Biopsy samples obtainedfrom these patients were used for culture, real-time polymerase chainreaction (RT-PCR), and histopathological examination. Stool sampleswere also collected from these patients and were tested using the Helicobacterpylori stool antigen (HpSA) kit. Histopathological examinationwas accepted as the gold standard test.Results: H. pylori was identified by histological examination in 77/87(87.5%) patients, whereas it was negative in 10/87 (12.5%) patients. Furthermore,positive results were obtained in 55 (63.2%), 71 (81.6%), and 77(87.5%) patients using the culture method, HpSA analysis, and RT-PCR method,respectively. The sensitivity and specificity of culture, HpSA, and PCRtests were determined as 71.4% and 100%, 87% and 60%, and 97.4% and80%, respectively. Antibiotic susceptibility tests were performed on 48 outof the 55 culture positive samples. Resistance to clarithromycin was foundin 28 (58.3%), metronidazole in 14 (29.2%), and levofloxacin in 4 (8.3%) ofthe isolates. Resistance to amoxicillin and tetracycline was not observed.Conclusion: There are currently several invasive and non-invasive diagnostictests for the detection of H. pylori infections. Each test has someadvantages and disadvantages. The diagnostic method of choice shouldbe easy and applicable to all age groups.Öğe Comparison of virulence factors and antibiotic resistance of Pseudomonas aeruginosa strains isolated from patients with and without cystic fibrosis(UNIV PRESS, 2017) Maçin, Salih; Akarca, Meral; Şener, Burçin; Akyön, YakutIts rising incidence, virulence factors and antibiotic resistance rate makes it difficult to treat Pseudomonas aeruginosa infections. The aim of this study was to compare virulence factors and antibiotic resistance of P. aeruginosa isolates from cystic fibrosis (CF) and other lower respiratory tract infections. Isolates from patients (n=125) were divided into two groups. The isolates in the first group were from CF patients (n=64). And in the other group isolates were from lower respiratory tract samples, from patients that did not have CF (n=61). The antibiotic susceptibility tests were done by using disc diffusion method. As phenotypic tests; DNase, protease, elastase, hemolysis, and motility test were performed. The mucoid form of P. aeruginosa was detected in 29.7% of CF patients' isolates, whereas in the other group (non-CF) this rate was 9.8% (p=0.011). Motility in the CF patients' isolates was lower (84.4%) then the other group (96.7%). The presence of DNase was significantly low in CF patients' isolates when compared to the other group (p=0.009). When the antibiotic resistance was compared; ceftazidime, imipenem and meropenem and piperacillin resistance was found significantly low in CF patients compared to isolates from the other group (p=0.05). Information about virulence factor patterns and antibiotic resistance of P. aeruginosa isolates from patients with cystic fibrosis and the patients without cystic fibrosis can prevent the unnecessary usage of antibiotics and lead the way to new approaches in treatment..